Explanation of different thickness estimates used for the simulation of repeatability.

<p>The red areas or points on the fundus images indicate the values that were averaged to generate the layer thickness estimates. The text to the right of each image refers to (top-to-bottom): 1) the used OCT device 2) the applied scan as elucidated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137316#pone.0137316.g001" target="_blank">Fig 1</a>; 3) the (subset of) B-scans used for the thickness estimate; 4) the procedure used to calculate the thickness estimates; 5) the program used for calculating the thickness estimate. The last column indicated the article(s) in which the layer estimated was applied. For a detailed overview of the segmentation methods applied in the retrieved articles, see Table B in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137316#pone.0137316.s002" target="_blank">S1 File</a>. Note: the number and spacing of B-scans on the fundus image for the thickness estimates C and E are not correct due to space limitations.</p

OCT scan settings used for simulation of the repeatability of different thickness estimates.

<p>The first column indicates the OCT device used to produce the scan in each row. The following column names each set of scanning parameter as a concatenation of scan direction, the number of B-scans and the number of A-scans (see columns 4–6). The third column shows for each scan design the generated OCT scans as lines or boxes on exemplary fundus images. The heights and widths of the scan areas are given in column 7, followed by the number of averaging frames in automatic real-time mode in the last column (only applicable for the Spectralis SD-OCT scan settings). NA = not applicable.</p

Intraclass correlation coefficients (ICC) for different thickness estimates and retinal layers in MS/CIS patients and healthy controls.

<p>Intraclass correlation coefficient (ICC) for the distinct cohorts of healthy controls (HC) and patients (MS) ordered for (A) the twelve thickness estimates (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137316#pone.0137316.g003" target="_blank">Fig 3</a>) and (B) the seven retinal layers. Abbreviations: mRNFL = macular retinal nerve fiber layer, GCL = ganglion cell layer, IPL = inner plexiform layer, GICP = combined GCL and IPL, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer.</p

Coefficient of repeatability (CR, in μm) of thickness estimates and retinal layers in a mixed cohort of healthy controls and MS/CIS patients.

<p><b>Abbreviations: mRNFL</b> = macular retinal nerve fiber layer, <b>GCL</b> = ganglion cell layer, <b>IPL</b> = inner plexiform layer, <b>GCIP</b> = combined GCL and IPL, INL = inner nuclear layer, <b>OPL</b> = outer plexiform layer, <b>ONL</b> = outer nuclear layer.</p><p>Coefficient of repeatability (CR, in μm) of thickness estimates and retinal layers in a mixed cohort of healthy controls and MS/CIS patients.</p

Coefficient of repeatability (CR) for different thickness estimates and retinal layers in MS/CIS patients and healthy controls.

<p>Coefficient of repeatability (CR) for the distinct cohorts of healthy controls (HC) and patients (MS) ordered for (A) the 12 thickness estimates (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137316#pone.0137316.g003" target="_blank">Fig 3</a>) and (B) the 7 retinal layers. <b>Abbreviations</b>: <b>mRNFL</b> = macular retinal nerve fiber layer, <b>GCL</b> = ganglion cell layer, <b>IPL</b> = inner plexiform layer, <b>GICP</b> = combined GCL and IPL, <b>INL</b> = inner nuclear layer, <b>OPL</b> = outer plexiform layer, <b>ONL</b> = outer nuclear layer.</p

Repeatability of thickness estimates and retinal layers in a mixed cohort of healthy controls and MS/CIS patients as measured by intraclass correlation coefficients (ICC) with 95%-confidence intervals.

<p><b>Abbreviations: mRNFL</b> = macular retinal nerve fiber layer, <b>GCL</b> = ganglion cell layer, <b>IPL</b> = inner plexiform layer, <b>GCIP</b> = combined GCL and IPL, INL = inner nuclear layer, <b>OPL</b> = outer plexiform layer, <b>ONL</b> = outer nuclear layer.</p><p>Repeatability of thickness estimates and retinal layers in a mixed cohort of healthy controls and MS/CIS patients as measured by intraclass correlation coefficients (ICC) with 95%-confidence intervals.</p