Polimorfizam gena OLR1 i funkcionalna svojstva mliječnih krava

The study analyzed the polymorphism of single nucleotide substitution A8232C, located in the 3’UTR region of the receptor of the oxidized low-density lipoprotein (OLR1) gene. The study was conducted in a herd of Polish Holstein-Friesian (Red-and-White strain) dairy cattle. Identification of genotypes of individuals was performed using PCR-RFLP. The study stated the following frequency of A8232C polymorphism alleles: A - 0.30 and C - 0.70. Statistical analysis showed that the AC genotype cows were characterized by higher milk yield, protein and fat and for fat yield, the difference was confirmed statistically (P≤0.05). The cows with genotype AA were characterized by the latest occurrence of first calving and the longest intercalving interval, but the results were not confirmed statistically.Analiziran je polimorfizam pojedinačnog nukleotida na poziciji A8232C, smještenog u regiji 3’UTR, gena za receptor oksidiranog lipoproteina male gustoće (OLR1). U stadu poljskih holštajnsko-frizijskih (crveni i bijeli soj) krava za mlijeko genotipovi jedinki identificirani su PCR-RFLP metodom. Ustanovljena je sljedeća učestalost A8232 polimorfnih alela: A - 0,30 i C - 0,70. Statistička je analiza pokazala da su krave s genotipom AC imale veću proizvodnju mlijeka, bjelančevina i masti, pri čemu je razlika za mast potvrđena kao signifikantna (P≤0,05). Krave s genotipom AA imale su kasno prvo teljenje i dugo međutelidbeno razdoblje, no ti rezultati nisu potvrđeni kao statistički signifikantni

Ghrelin gene polymorphism in dairy cattle

The aim of this experiment was to estimate possible associations between GHRL G375A genotypes and some milk performance traits (yields of milk, protein and fat, and protein and fat content). The study included Polish Holstein–Friesian strain Red-and-White cows. The ACRS and PCR-RFLP method was used to identification genotypes. GHRL G375A frequencies were as follow: AA – 0.86, AG- 0.14, and GG – was not found, while allele frequencies were: A – 0.93 and G – 0.07. In this study, no statistically significant correlation between GHRL genotypes and analyzed traits was found, however, a tendency to maintain a relationship of genotypes with milk production traits is shown

Investigation on relationships of the FABP3 and SLC27A3 genes with milk production traits in sheep

The aim of this study was to determine whether genetic differences in genes FABP3 and SLC27A3 may be related to the productivity and composition of sheep’s milk. A herd of 50 Slovak sheep breed Zoslachtena Valaska (Zošľachtená Valaška, in Slovak) was studied. The frequencies of the most common alleles were as follows: FABP3 SNP13 0.82, SLC27A3 C/T 0.64, SCL27A3 A/G 0.74. The results of the statistical analysis for polymorphism in the FABP3 (SNP13) gene showed that animals with the homozygous AA genotype had the highest content of fat, protein and solids in the milk of tested sheep. The analysis of the results for the SLC27A3 C/T polymorphism allowed us to conclude that the milk of animals with the homozygous TT genotype was characterized by the lowest content of fat, protein and solids and the highest content of lactose. In the case of SLC27A3 A/G polymorphism, sheep with the heterozygous genotype were characterized by the highest fat content in milk and the lowest content of protein and lactose. The milk of sheep with the heterozygous genotype FABP3 was characterized by the highest share of serum albumin, α+β – caseins and α-lactalbumin. In contrast, animals with the homozygous AA genotype were characterized by the highest content of κ-casein. The relationships between genotypes of the SLC27A3 C/T polymorphism showed that sheep with the heterozygous genotype were characterized by the lowest content of serum albumin, and the highest content of α+β – caseins in milk. The analysis conducted for the SLC27A3 A/G polymorphism demonstrated that animals with the AA genotype were characterized by the lowest content of serum albumin in milk. Because most of the results were not confirmed statistically, we should continue research using different breeds of sheep and herds with a larger number of animals

Effects of Single Nucleotide Polymorphisms in the SLC27A3 Gene on the Nutritional Value of Sheep Milk

The current research was undertaken to use the genetic potential of animals to obtain high-quality dairy products. Single nucleotide polymorphisms (SNPs) in SLC27A3 gene were identified in Zošľachtená valaška sheep using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Correlations between genotypes and milk composition and nutritional value were analysed This study showed that milk from sheep with TT genotype in the SNP4 locus was characterised by higher (p < 0.01) fat and dry matter content and lower lactose concentration, compared to sheep with AA and TA genotypes, respectively. Moreover, it was found that animals with GG genotype in SNP1 produced milk with higher C18:1n9c, C18:1n7t, CLA, and other unsaturated fatty acids (UFAs) content than sheep with TT. Additionally, milk from animals with CC at the SNP3 locus had significantly higher (p < 0.01) levels of UFAs than milk from sheep with other genotypes in the SNP3. In summary, it may be concluded that milk from animals with TT genotype of SNP4 is characterised by higher fat and dry matter content. Whereas, milk from sheep with GG in SNP1 and with CC in SNP3 is characterised by higher content of UFAs, which increases milk value as material for functional food production