EFFECT OF LEPTIN ON FOLLICULOGENESIS DURING DEVELOPMENT IN FEMALE WISTAR RAT

This study aims to investigate the effect of leptin on ovaries in female Wistar rat during development. Rats were divided into two groups: 30 and 60-day-old females. Experimental treated females were administrated                 8 μg/100 g body weight intraperitoneal leptin injections daily for 5 days, while controls received the same volume of saline solution. Leptin treatment resulted in a significant decrease in body weight (150.50 ± 4.02 vs 157.78 ± 3.51 g,               p 0.05). For FSH, the difference between controls and treated females was not significant in both ages (p > 0.05). Leptin appears to be involved in the regulation of body weight, folliculogenesis, the ovulatory process and luteogenesis by control of gonadotropic hormones and progesterone at the age of 60 days but not at 30 days, when leptin receptors are probably not expressed yet

EFFECT OF LEPTIN ON OVOGENESIS DURING DEVELOPMENT IN FEMALE WISTAR RAT

The present study aims to investigate the effect of leptin treatment on ovarian weight and ovarian follicle enumeration during development in female Wistar rats. The experiment is carried out on 30 and 60-day-old females. Leptin diluted in 9‰ NaCl is injected daily for 5 days intraperitoneally at a dose of 8 μg / 100 g of body weight to the experimental females. The same volume of the NaCl solution is injected into the controls. Animals are sacrificed 24 hours after the last injection. Ovaries are removed and weighed. The right ovaries are fixed in 10% formaldehyde for histological study. The results reveal a non-significant increase in absolute and relative mean weights of the right and left ovaries in leptin-treated groups compared to controls in both immature and adult rats. The histological study reveals that in 30-day-old females, the number of atretic follicles is most important compared to normal follicles after leptin-treatment, which suggests that leptin appears to stimulate cellular apoptosis at immature age. These results allow us to conclude that leptin seems to influence ovarian weight and follicular atresia