Phytoremediation of Lead Polluted Soil by Glycine max

A study was designed to assess the phytoextraction potential of Glycine max L. for lead (Pb). Pots experiment was conducted. Viable seeds were planted in 5 kg of soil placed in each plastic pot having 0 ppm (control), 5 ppm, 10 ppm, 15 ppm, 20 ppm and 25 ppm of Pb respectively. The study was carried out for a period of 12 weeks under natural conditions. Physicochemical properties of the soil were determined using standard methods. The results revealed that pH, phosphorous and moisture contents increased while nitrogen and organic carbon contents decreased in polluted soil remediated with Glycine max L. compared to the unpolluted soil. Leaf, stem, seeds and roots of the plant were analyzed for Pb uptake after 12 weeks. The plants mopped up substantial concentration of Pb in the above plant biomass of the seeds (4.2 mg/kg), stem (1.37 mg/kg) and leaves (3.37 mg/kg) compared to concentrations in the roots (1.53 mg/kg). The phytoextraction ability of the plant was assessed in terms of its bioconcentration factor (BCF) and translocation factor (TF). It was observed that the levels of Pb in the roots and shoots after 12 weeks showed that more bioavailable pool of Pb was translocated from the root to seeds, leaves and stem in that order. The results obtained suggest that the plant has phytoextraction ability and could be used in restoring soil polluted with Pb

Microbial-aided phytoremediation of heavy metals contaminated soil: a review

Anthropogenic exercises as well as industrial enterprise and agricultural practices contribute considerably to the degradation and contamination of environment that considerably affects the soil. The normal physical and chemical know-how soil washing used for soil remediation render the land useless as a medium for plant growth, as they take away all biological activities. Others are labor-intensive and have high maintenance value phytoremediation, a cheaper and sustainable in situ remediation technique was so thought of. This data can enable proposing solutions to issues of contamination and eventually convalescent sites and soils. However, plants don't have the aptitude to degrade several soil waste matters particularly the organic pollutant. It's so imperative to require advantage of the degrading ability of soil microorganisms. This review so focuses on phytoremediation techniques improved by microbial colonies. DOI: http://dx.doi.org/10.5281/zenodo.324417

Phytoremediation of Lead Polluted Soil by Glycine max L

A study was designed to assess the phytoextraction potential of Glycine max L. for lead (Pb). Pots experiment was conducted. Viable seeds were planted in 5 kg of soil placed in each plastic pot having 0 ppm (control), 5 ppm, 10 ppm, 15 ppm, 20 ppm and 25 ppm of Pb respectively. The study was carried out for a period of 12 weeks under natural conditions. Physicochemical properties of the soil were determined using standard methods. The results revealed that pH, phosphorous and moisture contents increased while nitrogen and organic carbon contents decreased in polluted soil remediated with Glycine max L. compared to the unpolluted soil. Leaf, stem, seeds and roots of the plant were analyzed for Pb uptake after 12 weeks. The plants mopped up substantial concentration of Pb in the above plant biomass of the seeds (4.2 mg/kg), stem (1.37 mg/kg) and leaves (3.37 mg/kg) compared to concentrations in the roots (1.53 mg/kg). The phytoextraction ability of the plant was assessed in terms of its bioconcentration factor (BCF) and translocation factor (TF). It was observed that the levels of Pb in the roots and shoots after 12 weeks showed that more bioavailable pool of Pb was translocated from the root to seeds, leaves and stem in that order. The results obtained suggest that the plant has phytoextraction ability and could be used in restoring soil polluted with Pb

PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANTS BY ISOLATES FROM LUBRICATING OIL POLLUTED SOIL

The study intended to isolate, Produce and characterize biosurfactant producing isolates from spent lubricating oilpolluted soil. The isolated bacteria were identified as Acinectobacter iwoffii mpe25 and Micrococcus kristinea mpe12. Both bacteria exhibited the ability in producing biosurfactants as evidenced by blood haemolysis, oil displacement, and drop collapse and emulsification activities. After the fermentation for seven days, biosurfactant from the two bacteria was successfully extracted from the fermentation by centrifugation and purified by solvent extraction using acid precipitation method. Characterization of the biosurfactants was done by thin-layer chromatography (TLC), gas chromatography and mass spectroscopy (GC-MS), and Fourier transforms infrared (FTIR) spectroscopy. The bacteria lysed red blood cells by forming cleared transparent halos (beta haemolysis) around the colonies caused the wide diameter of oil displacement of 5.5cm, 6.10cm, and high emulsification capacity of 74.5% and 65.0 % respectively. The GC-MS revealed the presence of palmitic acid, 15-hydroxypentadecanoic acid, 2, 3- dihydroxydodecanoic acid, sulfur-dodecyl-2-ethyl hexyl ester; the FTIR revealed important functional groups (S=O, C-O-C, C=O) that defined the biosurfactant to be a glycolipid with a unique sulfonyl group in both biosurfactants. These led to the assigned name of sulfoglycolipid Bios-25 and disulfoglycolipid Bios-12 to the biosurfactant produced by A. iwoffii mpe25 and M. kristinea mpe12 respectively. Due to the high emulsification capacity of these biosurfactants, it is recommended for use in bioremediation studies

Safety Evaluation of Mangifera Indica Bark for Raw Water Purification

Background: Synthetic coagulants commonly used for water treatment are associated with undesirable properties, such as inefficacy and toxicity in addition to being expensive. Natural coagulants are considered safe and economical alternatives for developing countries where the plants are abundantly available. Methods: The phytochemical composition and safety of water samples treated with Mangifera indica (M. indica) were evaluated in 13 groups of albino rats (N=36) for four weeks and compared with water samples treated with alum and calcium hypochlorite, using biochemical and hematological parameters. Results: Mangifera indica bark contained alkaloids, flavonoids, saponins, phenols, tannins, terpenes, steroids and cardiac glycoside. Both the raw water samples (rivers, ponds & streams) and treated waters (alum, calcium hypochlorite and plant material) did not caused any significant (p>0.05) changes to the activities or levels of transaminases (AST and ALT), alkaline phosphatase, total serum proteins, urea, creatinine, sodium, potassium, platelet and mean corpuscular hemoglobin concentration compared with those in normal control rats. Water samples treated with M. indica caused significant increases (p<0.05) in packed cell volume, hemoglobin, and red blood cells of the animals while the untreated water samples significantly increased the white blood cell. However, alum treated water significantly increased (p<0.05) the concentrations of serum urea, sodium and potassium while calcium hypochlorite treated water significantly (p<0.05) increased the creatinine and potassium concentrations. Conclusion: The use of M. indica bark in water purification confers hematopoietic properties to the water and reduces adverse effects on the biochemical parameters, thus could be considered as an effective and safe agent for water purification

Microbial-aided phytoremediation of heavy metals contaminated soil: a review

Anthropogenic exercises as well as industrial enterprise and agricultural practices contribute considerably to the degradation and contamination of environment that considerably affects the soil. The normal physical and chemical know-how soil washing used for soil remediation render the land useless as a medium for plant growth, as they take away all biological activities. Others are labor-intensive and have high maintenance value phytoremediation, a cheaper and sustainable in situ remediation technique was so thought of. This data can enable proposing solutions to issues of contamination and eventually convalescent sites and soils. However, plants don't have the aptitude to degrade several soil waste matters particularly the organic pollutant. It's so imperative to require advantage of the degrading ability of soil microorganisms. This review so focuses on phytoremediation techniques improved by microbial colonies. DOI: http://dx.doi.org/10.5281/zenodo.324417

Microbiological, Nutritional, and Sensory Quality of Bread Produced from Wheat and Potato Flour Blends

Dehydrated uncooked potato (Irish and sweet) flour was blended by weight with commercial wheat flour at 0 to 10% levels of substitution to make bread. Comparative study of the microbial and nutritional qualities of the bread was undertaken. The total aerobic bacterial counts ranged from 3.0 × 10 5 cfu/g to 1.09 × 10 6 cfu/g while the fungal counts ranged from 8.0 × 10 1 cfu/g to 1.20 × 10 3 cfu/g of the sample. Coliforms were not detected in the bread. Bacteria isolated were species of Bacillus, Staphylococcus, and Micrococcus while fungi isolates were species of Aspergillus, Penicillium, Rhizopus, and Mucor. The mean sensory scores (color, aroma, taste, texture, and general acceptability) were evaluated. The color of the bread baked from WF/IPF 2 (wheat/Irish potato flour, 95 : 5%) blend was preferred to WF (wheat flour, 100%) while WF/SPF 1 (wheat/sweet potato flour, 100%) and WF/IPF 1 (wheat/Irish potato flour, 90 : 10%) aroma were preferred to WF. However, the bread baked from WF, WF/IPF 2 (wheat flour/Irish potato flour, 95 : 5%), and WF/SPF 2 (wheat/sweet potato flour, 95 : 5%) was more acceptable than other blends. The use of hydrated potato flour in bread making is advantageous due to increased nutritional value, higher bread yield, and reduced rate of staling