Unravelling the genome of the brackish water malaria vector Anopheles aquasalis

Abstract Malaria is a severe public health problem in several developing tropical and subtropical countries. Anopheles aquasalis is the primary coastal malaria vector in Central and South America and the Caribbean Islands, and it has the peculiar feature of living in water with large changes in salinity. Recent research has recognised An. aquasalis as an important model for studying the interactions of murine and human Plasmodium parasites. This study presents the complete genome of An. aquasalis and offers insights into its evolution and physiology. The genome is similar in size and gene content to other Neotropical anophelines, with 162 Mb and 12,446 protein-coding genes. There are 1387 single-copy orthologs at the Diptera level (eg. An. gambiae, An. darlingi and Drosophila melanogaster). An. aquasalis diverged from An. darlingi, the primary malaria vector in inland South America, nearly 20 million years ago. Proteins related to ion transport and metabolism belong to the most abundant gene families with 660 genes. We identified gene families relevant to osmosis control (e.g., aquaporins, vacuolar-ATPases, Na+/K+-ATPases, and carbonic anhydrases). Evolutionary analysis suggests that all osmotic regulation genes are under strong purifying selection. We also observed low copy number variation in insecticide resistance and immunity-related genes for all known classical pathways. The data provided by this study offers candidate genes for further studies of parasite-vector interactions and for studies on how anophelines of brackish water deal with the high fluctuation in water salinity. We also established data and insights supporting An. aquasalis as an emerging Neotropical malaria vector model for genetic and molecular studies

Performance of Cowpea under Different Water Regimes in Amazonian Conditions

Water availability is a crucial factor in the final productivity of cowpea. The objective of this work was to evaluate the production and productivity components of cowpea under different irrigation depths in Amazonian conditions. The experiment was carried out at the School Farm of the Federal Rural University of Amazonia, in the municipality of Castanhal-PA, using the cultivar BR3 Tracuateua, from September to November 2014, 2015, and 2016. The experimental design was conducted on six blocks and four treatments, where the four irrigation depths of 0, 25, 50, and 100% of crop evapotranspiration were tested. The productivity analysis was performed when 90% of the plants were in the phenological stage R9. The evaluated production components were pod length, number of pods per plant, number of grains per pod, mass of one hundred grains, and harvest index. There was a statistical difference among all treatments for the components of production and among productivities. An average reduction of 827 kg ha−1 in cowpea productivity was observed during the three years of study, when the treatment without irrigation was compared with the treatment irrigated with 100% of the crop’s water demand. It was found in this research that the simple fulfillment of the nutritional and phytosanitary demands of the crop, associated with an adequate planning of when to plant in the region, would already help in the improvement of local production when choosing times where the water deficit in the reproductive phase is less than 33 mm

Performance of Cowpea under Different Water Regimes in Amazonian Conditions

Water availability is a crucial factor in the final productivity of cowpea. The objective of this work was to evaluate the production and productivity components of cowpea under different irrigation depths in Amazonian conditions. The experiment was carried out at the School Farm of the Federal Rural University of Amazonia, in the municipality of Castanhal-PA, using the cultivar BR3 Tracuateua, from September to November 2014, 2015, and 2016. The experimental design was conducted on six blocks and four treatments, where the four irrigation depths of 0, 25, 50, and 100% of crop evapotranspiration were tested. The productivity analysis was performed when 90% of the plants were in the phenological stage R9. The evaluated production components were pod length, number of pods per plant, number of grains per pod, mass of one hundred grains, and harvest index. There was a statistical difference among all treatments for the components of production and among productivities. An average reduction of 827 kg ha−1 in cowpea productivity was observed during the three years of study, when the treatment without irrigation was compared with the treatment irrigated with 100% of the crop’s water demand. It was found in this research that the simple fulfillment of the nutritional and phytosanitary demands of the crop, associated with an adequate planning of when to plant in the region, would already help in the improvement of local production when choosing times where the water deficit in the reproductive phase is less than 33 mm