Effect of ultrasonic activation of endodontic sealers on the tubule penetration and on the bond strenght to root dentin

O objetivo deste estudo foi avaliar o efeito da ativação ultrassônica de cimentos endodônticos na penetração intratubular e na resistência de união à dentina radicular. Os canais de 100 dentes monorradiculares foram preparados com instrumentos de níquel-titânio e irrigados com hipoclorito de sódio 2,5% e EDTA 17% e obturados pela técnica de condensação lateral utilizando os seguintes cimentos: cimento à base de resina de salicilato MTA Fillapex (n = 20), cimento biocerâmico Sealer Plus BC (n = 20), e Endosequence BC (n = 20) e cimento à base de resina epóxica Sealer Plus (n = 20) e AH Plus (n = 20). Cada um destes grupos foi dividido em dois subgrupos (n = 10) de acordo com a ativação ultrassônica (20 segundos) ou não. Aos cimentos biocerâmicos, foi incorporado o corante Fluo-3 na proporção de 0,1%. Para os demais cimentos, rodamina B foi utilizada na mesma proporção. Os dentes foram seccionados transversalmente para análise de penetração intratubular e realização do teste de resistência de união. Os dados relativos à penetração intratubular foram analisados estatisticamente pelos testes T, ANOVA 1 fator Bonferroni. Os valores de resistência de união pelos testes T, Kruskal-Wallis e Dunn. O uso do ultrassom aumentou a penetração intratubular de todos os cimentos, porém com diferença significativa apenas para os grupos MTA Fillapex e Endosequence (P<0,05). Após ativação ultrassônica, o MTA Fillapex apresentou os maiores valores de penetração intratubular (P<0,05), sem diferenças entre os cimentos biocerâmicos e à base de resina epóxica (P˃0,05). Os valores de resistência de união dos cimentos AH Plus e Sealer Plus BC aumentaram após ativação ultrassônica (P<0,05). Após o uso do ultrassom, os cimentos à base de resina epóxica (AH Plus e Sealer Plus) e o biocerâmico Sealer Plus BC apresentaram os maiores valores de resistência de união (P<0,05). Houve um predomínio de falhas adesivas após o teste de push-out para todos os grupos. A ativação ultrassônica aumentou a penetração intratubular e influenciou na resistência de união à dentina radicular dos cimentos endodônticos avaliadosThis study aimed to evaluate the effect of ultrasonic activation of endodontic sealers on the dentin tubule penetration and on the push-out bond strength to root dentin. One hundred monorradicular teeth were prepared with niquel-titanium instruments irrigated with 2.5% sodium hypochlorite and 17% EDTA and filled using cold lateral compaction using the following selaers: a salycilate resin-based sealer (MTA Fillapex, n = 20), two bioceramic sealers (Sealer Plus BC and Endosequence, n = 20 for each group) and two epoxy resin-based sealers (AH Plus and Sealer Plus, n = 20 for each group). Next, the groups were subdivided according to the use or not of ultrasonic activation of the sealer (20 seconds). Fluo-3 fluorophore were added to the bioceramic sealers and rodhamine B to the other sealers. Both at a ratio of 0.1%. The teeth were transversely sectioned to evaluate the dentin tubule penetration and the bond strength to root dentin. The penetration data were analyzed with T-test and 1-factor ANOVA and Bonferroni tests. Bond strength was evaluated usin T-test and Kruskall-Wallis and Dunn post hoc test. The ultrasonic activation enhanced the dentine tubule penetration for all the sealers, but only for MTA Fillapex and Endosequence the difference was significant (p<0.05). After ultrasonic activation, MTA Fillapex showed the highest tubule penetration area (p<0.05), but with no differences among the other sealers (p˃0.05). The bond strength values of AH Plus and Sealer Plus BC enhanced after ultrasonic activation (p<0.05). Moreover, after ultrasonic activation the epoxy resin-based sealers (AH Plus and Sealer Plus) and the bioceramic Sealer Plus BC presented the highest bond strength values (p<0.05). Adhesive failures were predominant in all groups, regardless of the ultrasonic activation. The ultrasonic activation increased intratubular penetration and influenced the bond strength to root dentin of the evaluated endodontic sealers

Effect of ultrasonic activation of endodontic sealers on intratubular penetration and bond strength to root dentin

Introduction: This study aimed to evaluate the effect of ultrasonic activation (UA) of endodontic sealers on dentin tubule penetration and the bond strength to root dentin. Method: One hundred single-rooted teeth were prepared with 40.06 nickel-titanium instruments and divided into 2 groups: with or without UA. Three resin-based sealers (MTA Fillapex [Angelus Dental Solutions, Londrina, PR, Brazil], Sealer Plus [MK Life Medical and Dental Products, Porto Alegre, RS, Brazil], and AH Plus [Dentsply, DeTrey GmbH, Konstanz, Germany]; n 5 20) and 2 calcium silicate–based sealers (Sealer Plus BC [MK Life Medical and Dental Products] and EndoSequence BC [Brasseler, Savannah, GA], n 5 20) were used and subdivided (n 5 10) according to the protocols. Fluo-3 (Thermo Fisher Scientific, Waltham, MA) and rhodamine B dyes were added to the calcium silicate- and resin-based sealers, respectively. In the UA groups, the activation was performed for 40 seconds followed by lateral compaction. Samples were transversely sectioned to evaluate the dentin tubule penetration and the bond strength to root dentin. The penetration data were analyzed with the Student t test, 1-factor analysis of variance, and Bonferroni tests. Bond strength was eval- uated using the Student t test, Kruskal-Wallis, and Dunn post hoc test. Results: Resin-based sealers showed the highest tubule penetration without UA (P , .05). UA significantly enhanced MTA Fillapex and Endosequence BC dentin tubule penetration (P , .05). AH Plus and Sealer Plus BC improved their bond strength to root dentin after UA (P , .05). AH Plus/ UA, Sealer Plus/UA, and Sealer Plus BC/UA presented the highest bond strength values (P , .05). Adhesive failures were predominant in all groups regardless of the use of ultrasound. Conclusions: UA interferes with tubule penetration and the bond strength to root dentin of resin- and calcium silicate–based sealers

Intratubular penetration of endodontic sealers depends on thefluorophore used for CLSM assessment

Root canal filling aims at eliminating empty spaces into the root canal system usingbiologically compatible materials. Three-dimensional root canal obturation mustprevent or minimize the reinfection caused by microorganisms' leakage. This studyaimed at evaluating whether fluorophore (Rhodamine or Fluo-3) influences theCLSM images of intratubular penetration of four endodontic sealers. Eighty bovineteeth were prepared using K files up to a size #70 and irrigated with 2.5% sodiumhypochlorite. All roots were divided into eight groups (n= 10) according to thesealer and fluorophore used: AH Plus/Rhodamine, AH Plus/Fluo-3, Sealer Plus/Rhodamine, Sealer Plus/Fluo-3, Sealer Plus BC/Rhodamine, Sealer Plus BC/Fluo-3,Endosequence/Rhodamine, and Endosequence/Fluo-3. All roots were filled usingcold lateral compaction technique. After 7 days, the roots were transversely sec-tioned, and three slices, one of each canal third, were obtained. Intratubular pene-tration was evaluated using CLSM. Sealer Plus BC/Rhodamine and EndosequenceBC/Rhodamine presented higher intratubular penetration than AH Plus/Fluo-3 andSealer Plus/Fluo-3 (p< .05). The intragroup analysis showed similar intratubularpenetration, regardless of the root third, except for the apical third in AH Plus/Fluo-3 and Sealer Plus BC/Fluo-3 groups. The type of fluorophore influences thecalcium silicate sealers' tubular penetration but not of epoxy resin-based ones usingCLSM. Bioceramic sealers should not be used associated with Rhodamine for CLSMevaluation.Research Highlights:The type of fluorophore influences the calcium silicate sealers'tubular penetration but not of epoxy resin-based ones when CLSM is used forassessment. Bioceramic sealers should not be used associated with Rhodamine

INFLUENCE OF DIFFERENT ENDODONTIC SEALERS ON FIBERGLASS POST ADHESION

The aim of this study was to evaluate the influence of different endodontic sealers on fiberglass posts adhesion, using conventional and self-adhesive resin cements. Sixty single-rooted human teeth were selected. Canals were prepared with X1Blue 40.06 (MK Life). Roots were randomly distributed in six groups (n=10) as follows: G1) AH Plus/RelyX ARC; G2) AH Plus/RelyX U200; G3) BioC Sealer/RelyX ARC; G4) BioC Sealer/RelyX U200; G5) Sealer Plus BC/RelyX ARC; G6) Sealer Plus BC/RelyX U200. The roots were perpendicularly sectioned and three slices per root were obtained, each corresponding to a post third (cervical, middle and apical). Push-out test was performed. To analyze the failure pattern, specimens were analyzed under an optical microscope at 10× magnification. Bond strength was calculated and analyzed using ANOVA and Tukey tests with a significance level of 5%. The bond strength values of posts cemented with conventional resin cement (RelyX ARC) or with self-adhesive resin cement (RelyX U200) previously filled with AH Plus were similar (P &gt; 0.05). The lowest bond strength values were observed for the groups in which bioceramic sealers and conventional resin cement were associated (Bio C Sealer/RelyX ARC and Sealer Plus BC/RelyX ARC) (P &lt; 0.05). Adhesive failures between cement and post, and between cement and dentin were predominant (55.3%). Bioceramic sealers negatively influenced the bond strength values of fiber posts cemented with conventional resin cement

Effect of bioceramic root canal sealers on the bond strength of fiber posts cemented with resin cements

This study aimed to evaluate the influence of calcium silicate-based sealers on the bond strength of fiber posts using conventional and self-adhesive resin cement. Sixty single-rooted teeth were selected. The canals were prepared with a reciprocating instrument 40.06. The roots were randomly distributed in six groups (n = 10) according to the strategies for root canal filling and fiber posts cementation: AH Plus/RelyX ARC; AH Plus/RelyX U200; Bio C Sealer/RelyX ARC; Bio C Sealer/RealyX U200; Sealer Plus BC/RelyX ARC; and Sealer Plus BC/RelyX U200. The roots were transversally sectioned, and one slice per post-third was obtained. The push-out test was performed at a crosshead speed of 1mm/min. The failure patterns were described after assessment with a stereomicroscope with a 10× magnification. Bond strength was calculated and analyzed using the ANOVA and Tukey test. AH Plus did not influence the bond strength of fiber posts cemented with conventional (RelyX ARC) or with self-adhesive resin cement (RelyX U200). The lowest bond strength values were obtained when calcium silicate-based sealers were associated with conventional resin cement (Bio C Sealer/RelyX ARC and Sealer Plus BC/RelyX ARC). Except for Sealer Plus BC/RelyX ARC, all groups presented lower bond strength at the apical portion compared to the cervical portion of the post. Adhesive failures between cement and post and cement and dentin were predominant (55.3%). Calcium silicate-based sealers decreased the bond strength of fiber posts cemented with conventional resin cement.Este estudo teve como objetivo avaliar a influência de cimentos à base de silicato de cálcio na resistência de união de pinos de fibra utilizando cimentos resinosos convencionais e autoadesivos. Sessenta dentes humanos monorradiculares foram selecionados. Os canais foram preparados um instrumento reciprocante 40.06. As raízes foram distribuídas aleatoriamente em seis grupos (n = 10) de acordo com as estratégias de obturação do canal e cimentação dos pinos de fibra: AH Plus/RelyX ARC; AH Plus/RelyX U200; Bio C Sealer/RelyX ARC; Bio C Sealer/RealyX U200; Sealer Plus BC/RelyX ARC; e Sealer Plus BC/RelyX U200. As raízes foram seccionadas transversalmente e foi obtido um espécime por terço. O teste de push-out foi realizado em uma velocidade de 1mm/min. Os padrões de falha foram descritos após avaliação em estereomicroscópio com aumento de 10×. A resistência de união foi calculada e analisada por ANOVA e teste de Tukey. AH Plus não influenciou na resistência de união dos pinos de fibra cimentados com cimento resinoso convencional (RelyX ARC) ou autoadesivo (RelyX U200) (p > 0,05). Os menores valores de resistência de união foram obtidos quando os cimentos à base de silicato de cálcio foram associados ao cimento resinoso convencional (Bio C Sealer/RelyX ARC e Sealer Plus BC/RelyX ARC) (p < 0,05). Com exceção do Sealer Plus BC/RelyX ARC, todos os grupos apresentaram menor resistência de união na porção apical em comparação com a porção cervical do pino (p < 0,05). Falhas adesivas entre cimento e pino e cimento e dentina foram predominantes (55,3%). Cimentos a base de silicato de cálcio diminuíram a resistência de união de pinos de fibra cimentados com cimento resinoso convencional

Effect of ultrasonic activation of the adhesive system on dentin tubule penetration and the pushout bond strength of fiber posts

Statement of problem. Whether ultrasonic activation of the adhesive system improves dentin tubule penetration and the bond strength of fiber posts to root dentin is unclear. Purpose. The purpose of this in vitro study was to evaluate the effect of ultrasonic activation of 2 adhesive systems (etch-and-rinse and self-etch) and 1 glass ionomer cement on the dentin tubule penetration and pushout bond strength of fiber posts to root dentin. Material and methods. Sixty maxillary central incisors were endodontically treated and divided into 6 groups (n=10) as per the post cementation strategy: etch-and-rinse, etch-and-rinse and ultrasonic, self-etch, self-etch and ultrasonic, glass ionomer cement, and glass ionomer cement and ultrasonic. The primers, the adhesives, and the glass ionomer cement were activated for 20 seconds each, and the fiber posts were cemented with a resin cement. Dentin tubule penetration was evaluated by confocal laser scanning microscopy and the pushout bond strength measured at 3 post locations: cervical, middle, and apical. The failure patterns were also described after pushout testing. Results. Self-etch and ultrasonic showed higher dentin tubule penetration than the other cementation strategies (P<.05) and improved the bond strength values (P<.05), which were higher than etch-and-rinse and ultrasonic and glass ionomer cement and ultrasonic (P<.05). Adhesive failures at the cement and dentin interface were predominant in the etch-and-rinse, self-etch, and self-etch and ultrasonic groups. Conclusions. Ultrasonic activation improved the dentin tubule penetration of a self-etch adhesive system. The bond strength of fiber posts cemented with a self-etch adhesive system and a resin cement was improved after ultrasonic activation

Taxa de publicação de resumos da área de Endodontia apresentados na Sociedade Brasileira de Pesquisa Odontológica entre 2013 e 2016

Introdução: O conceito de Odontologia baseada em evidências requer a produção e publicação de pesquisas de alta qualidade em periódicos internacionais criteriosos. Objetivo: O presente estudo teve como objetivo avaliar as taxas de publicação de resumos endodônticos apresentados na reunião da SBPqO nos anos de 2013, 2014, 2015 e 2016. Material e Métodos: O fator de impacto e a classificação Qualis dos periódicos, e o tempo decorrido entre o congresso e a publicação completa também foram avaliados. Em seguida, foram classificados em seis categorias: pesquisa básica endodôntica, revisão de temas endodônticos, estudos clínicos, ensaios clínicos randomizados, pesquisa básica tecnológica ou relatos de caso. Uma pesquisa em bancos de dados eletrônicos usando o mecanismo Medline (PubMed) foi realizada de janeiro de 2018 a julho de 2018, para identificar publicações escritas em inglês com texto completo dos resumos endodônticos previamente selecionados. A identificação da publicação em texto completo dos resumos foi inicialmente realizada utilizando o sobrenome do primeiro autor e as principais palavras-chave do título do resumo. Após a verificação do artigo em texto completo, informações adicionais foram registradas, tais como: tempo decorrido até a publicação; nome, fator de impacto e classificação Qualis da revista; e o tipo de estudo. Resultados: Após a leitura do conteúdo da reunião, 1.238 resumos (10,2%) foram considerados de interesse endodôntico e, em seguida, foi realizada uma busca eletrônica no banco de dados. Conclusão: Um grande número de resumos endodônticos não foi publicado como um manuscrito completo. Estudos clínicos e ensaios clínicos randomizados representaram uma pequena parte das publicações