Phylogenetic relationship of the polymerase protein of the Mapputta Group viruses and other selected orthobunyaviruses.

<p>Relationship was inferred by Bayesian analysis of the protein sequence alignment. A WAG model of aa substitution with gamma+invariant site heterogeneity was used. Numbers represent Bayesian posterior probabilities (Maximum Likelihood Bootstrap values). Tomato spotted wilt virus (TSWV) has been included as an out-group. Tree is drawn to scale measured in substitutions/site as indicated by the scale bar.</p

Map of Australia and Papua New Guinea showing geographical locations of the first isolations of MAPV, MPKV and BUCV.

<p>MAPV was isolated in 1960 from <i>Anopheles meraukensis</i> mosquitoes near the Mitchell River Mission in Queensland and MPKV was isolated in 1966 from <i>Aedes funereus</i> mosquitoes at the Southern foothills of the Prince Alexander Range near Maprik, Papua New Guinea. BUCV was isolated from <i>Anopheles meraukensis</i> mosquitoes in Darwin, Northern Territory in 1982. First isolation locations of TRUV, GGV, SASHV and MURBV are shown for comparison.</p

Phylogenetic relationship of the M segment polyprotein of the Mapputta Group viruses and other selected orthobunyaviruses.

<p>Relationship was inferred by Bayesian analysis of the protein sequence alignment. A WAG model of aa substitution with gamma+invariant site heterogeneity was used. Numbers represent Bayesian posterior probabilities (Maximum Likelihood Bootstrap values). Tomato spotted wilt virus (TSWV) has been included as an out-group. Tree is drawn to scale measured in substitutions/site as indicated by the scale bar.</p

Genome terminal sequences of MAPV, MPKV and BUCV compared with the orthobunyavirus type species, BUNV.

<p>MAPV and BUCV have the universally conserved complementary terminal 11 bases (in bold) typically observed in orthobunyaviruses, including a non-canonical base pairing at position 9 (<u>underlined</u>). MPKV differs from the consensus sequence with an additional non-canonical base pairing at position 8 (<u>underlined</u>).</p

Cross neutralisation of viruses of the Mapputta group.

<p>Cross neutralisation of viruses of the Mapputta group.</p

Phylogenetic relationship of the nucleocapsid protein of the Mapputta Group viruses and other selected orthobunyaviruses.

<p>Relationship was inferred by Bayesian analysis of the protein sequence alignment. A WAG model of aa substitution with gamma+invariant site heterogeneity was used. Numbers represent Bayesian posterior probabilities (Maximum Likelihood Bootstrap values). Tomato spotted wilt virus (TSWV) has been included as an out-group. Tree is drawn to scale measured in substitutions/site as indicated by the scale bar.</p

Genome segments of MAPV, MPKV and BUCV.

<p>Murrumbidgee virus (MURBV, GenBank accession numbers KF234253–5) and Salt Ash virus (SASHV, GenBank accession numbers KF234256–8) have been included for comparison.</p><p>N/A: Not available;</p><p>*incomplete genome sequence.</p><p>Genome segments of MAPV, MPKV and BUCV.</p

Isolation and seroprevalence information for Mapputta, Maprik and Buffalo Creek viruses.

<p>QIMR, Queensland Institute of Medical Research; ANU, Australian National University; BVL, Berrimah Veterinary Laboratory.</p><p>*Previously published data [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116561#pone.0116561.ref005" target="_blank">5</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116561#pone.0116561.ref007" target="_blank">7</a>].</p><p>Isolation and seroprevalence information for Mapputta, Maprik and Buffalo Creek viruses.</p

Nucleotide and amino acid identity comparisons for the Mapputta Group viruses and representative orthobunyaviruses.

<p>Nucleotide (bottom left) and amino acid (top right) identity values of sequences from the complete N ORF (S segment), polyprotein ORF (M segment) and RdRp ORF (L segment) of the Mapputta group viruses. Viruses of the Bunyamwera (Bunyamwera (BUNV), Wyeomyia (WYOV)), Simbu (Oropouche, OROV) and California (LaCrosse, (LACV), Jamestown Canyon (JCV) serogroups are also included for comparison.</p><p>Nucleotide and amino acid identity comparisons for the Mapputta Group viruses and representative orthobunyaviruses.</p