Comparison of mutation spectra induced by gamma-rays and carbon ion beams

The ionizing radiation with high linear energy transfer (LET), such as a heavy ion beam, induces more serious biological effects than low LET ones, such as gamma- and X-rays. This indicates a difference in the DNA damage produced by low and high LET radiations and their biological effects. We have been studying the differences in DNA damage produced by gamma-rays and carbon ion beams. Therefore, we analyze mutations induced by both ionizing radiations to discuss the differences in their biological effects in this study. pUC19 plasmid DNA was irradiated by carbon ion beams in the solution containing 1M dimethyl sulfoxide to mimic a cellular condition. The irradiated DNA was cloned in competent cells of Escherichia coli. The clones harboring some mutations in the region of lacZ alpha were selected, and the sequence alterations were analyzed. A one-deletion mutation is significant in the carbon-irradiated DNA, and the C:G T:A transition is minor. On the other hand, the gamma-irradiated DNA shows mainly G:C T:A transversion. These results suggest that carbon ion beams produce complex DNA damage, and gamma-rays are prone to single oxidative base damage, such as 8-oxoguanine. Carbon ion beams can also introduce oxidative base damage, and the damage species is 5-hydroxycytosine. This was consistent with our previous results of DNA damage caused by heavy ion beams. We confirmed the causal DNA damage by mass spectrometry for these mutations

ITを活用した自殺予防プログラムに関する臨床心理学的研究

研究期間:平成16-18年度 ; 研究種目:基盤研究C ; 課題番号:1653045

大学キャンパスにおける事件・事故等への危機対応システムに関する臨床心理学的研究

研究期間:平成19-21年度 ; 研究種目:基盤研究C ; 課題番号: 1953062

調理における段取り力を向上させる授業モデルの開発

application/pdf調理を実践する習慣の定着のためには、「調理が面倒」という意識の解消が必要であり、これには調理を効率よく行うことができる段取り力を持つことが有効である。そこで「調理における段取り力を向上させるプログラム」を作成し、段取り力向上の手立ての有効性を検証した。その結果、作成したプログラムの有効性が明らかになった。なお、この有効性は、調理における段取り力の定義を明確化した上で作成した客観的指標により評価したものである。In order to establish the practice of cooking, it is necessary to eliminate the awareness that "cooking is troublesome", and for this purpose , it is effective to have a setup power that can perform cooking efficiently. Therefore, we created a "program to improve the preparation ability in cooking" and verified the effectiveness of measures to improve the preparation ability. As a result, the effectiveness of the created program became clear. In addition, this effectiveness is evaluated by the cbjective index created after clarifying the definition of the preparation ability in cooking.2016年度～2018年度科学研究費補助金(基盤研究(C))研究成果報告書16K0475