Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture

For the assay of the production of Interleukin 2 (IL-2) in human peripheral blood lymphocytes (human PBL), a study was made on the optimal condition for its culture. 1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5% 2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture. 3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS

PSK 投与によるヒトの各種免疫学的パラメーターの変動

PSK was administered to the retired workers of the Poison Gas factory who showed high incidence of malignancy and immunological abnormalities. Furthermore, changes of various immunological parameters were studied. In the group in which Leu 1 positive rate showed a low value, Leu 1 positive rate showed significant increase after PSK administration. NK cell activity as well as Leu 7 positive rate showed significant elevation after PSK administration. It seems that PSK potentiates the immunity of non-tumor bearing individuals with depressed immunity