Intravitreal triamcinolone acetonide for rebound phenomenon after high-dose intravenous steroid treatment in Vogt-Koyanagi-Harada disease

Ik Soo Byon1, Ji Hun Kim1, Ji Eun Lee1,2, Boo Sup Oum1,2 1Department of Ophthalmology, Pusan National University Hospital; 2Medical Institute, School of Medicine, Pusan National University, Busan, Republic of Korea Abstract: The authors report two cases of rebound phenomenon treated with intravitreal triamcinolone acetonide in Vogt-Koyanagi-Harada (VKH) disease. Patients in the acute phase of VKH disease were treated with high-dose intravenous (IV) methylprednisolone (1 g/day) for 3 days. Serous retinal detachment decreased and visual acuity improved during IV steroid treatment. After switching to oral steroid treatment, choroiditis and visual acuity worsened. An injection of triamcinolone acetonide (4 mg) into the vitreous resulted in gradual resolution of subretinal fluid and improvement of visual acuity. Systemic steroids were tapered to discontinuation without a relapse of inflammation. Adjuvant intravitreal triamcinolone is useful in the management of the rebound phenomenon in VKH disease. Keywords: adjuvant intravitreal steroid, serous retinal detachment, visual acuity, choroiditi

A noncovalent approach to the construction of tween 20-based protein microarrays

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A case of presumed acute retinal necrosis after intraocular foreign body injury

Sung Who Park,1 Ik Soo Byon,1 Hyun Jun Park,2 Ji Eun Lee,1,3 Boo Sup Oum1,31Department of Ophthalmology, School of Medicine, Pusan National University, Busan, Korea; 2Department of Ophthalmology, Yangsan Pusan National University Hospital, Busan, Korea; 3Medical Research Institute, School of Medicine, Pusan National University Hospital, Busan, KoreaAbstract: The aim of this study was to report a case of acute retinal necrosis (ARN) after intraocular foreign body removal. A 32-year-old male presented with visual loss in the left eye. He was hit by an iron fragment while he was hammering. An intraocular foreign body was found with corneal laceration and traumatic cataract. On the day he was injured, primary closure of the laceration, lensectomy, and vitrectomy were performed, and the foreign body was removed. The day after the operation, there was no sign of retinal detachment or retinitis. Two days after the operation, retinal necrosis and accompanying vitreous inflammation were noted in the far periphery. On  day 3, the necrosis spread circumferentially and inflammation became more distinct. ARN was presumed and intravenous acyclovir was administered. The necrotic areas were reduced 2 days later, and were resolved in 1 month. The final visual acuity in his left eye was 20/20 after implantation of an intraocular lens. This case is the first report of ARN after penetrating injury and an intraocular foreign body. ARN may develop after open-globe injury.Keywords: necrotizing herpetic retinopathy, acute retinal necrosis, intraocular foreign bod

Polymeric Rulers: Distance-Dependent Emission Behaviors of Fluorophores on Flat Gold Surfaces and Bioassay Platforms Using Plasmonic Fluorescence Enhancement

In this paper, we introduce the concept of a "polymeric ruler" for investigating distance-dependent emission behaviors of fluorophores, namely the quenching or enhancement of fluorescence, on flat Au surfaces in the range of 5 to similar to 100 nm, which has not previously been easily accessible. The polymeric ruler is constructed by a highly controllable surface-initiated atom transfer radical polymerization of oligo(ethylene glycol)methacrylate (OEGMA), and the obtained thicknesses of the poly(OEGMA) (pOEGMA) layers range from similar to 5 to similar to 80 nm. The quenching or enhancement of fluorescence is found to be dependent upon the distance between fluorophores and the Au surface. In brief, fluorescence quenching occurs at distances within about 15 nm from the Au surface, and surface-enhanced fluorescence is observed at tens of nanometers beyond the range of quenching with the maximum enhancement at about 40-50 nm. The obtained information on the distance-dependent surface-enhanced fluorescence is applied to the construction of highly sensitive bioassay platforms: the use of the 50 nm thick pOEGMA layer lowers the detection limit up to 1 pM.X1125sciescopu