36 research outputs found
SECRETION OF HETEROLOGOUS PROTEINS BY ASPERGILLUS-NIGER - PRODUCTION OF ACTIVE HUMAN INTERLEUKIN-6 IN A PROTEASE-DEFICIENT MUTANT BY KEX2-LIKE PROCESSING OF A GLUCOAMYLASE-HIL6 FUSION PROTEIN.
No full textAn Escherichia coli Differential Killing Test for Carcinogens Based on a uvrA recA lexA Triple Mutant
No full textHighly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance
No full textExpression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi
No full textA chimaeric β-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Cotransformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke)) resulted in the expression of β-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi
Establishing an efficient gene-targeting system in an itaconic-acid producing Aspergillus terreus strain
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New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins; genetical and biochemical characterization of seven complementation groups
No full textThe effects of deletion of cellobiohydrolase genes on carbon source-dependent growth and enzymatic lignocellulose hydrolysis in Trichoderma reesei
No full textEffect of recB21, uvrD3, lexA101 and recF143 mutations on ultraviolet radiation sensitivity and genetic recombination in ΔuvrB strains of Escherichia coli K-12
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