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Protection Against Highly Pathogenic SIV by BCG-SIV Recombinant Priming and Attenuated Replicating Vaccinia-SIV Recombinant Boosting
Virus-Induced Gene Silencing (VIGS) in Cassava Using Geminivirus Agroclones.
peer reviewedVirus-induced gene silencing (VIGS) is an efficient, low-cost, and rapid functional validation tool for candidate genes in planta. The VIGS approach is particularly suitable to perform reverse genetics studies in crop species. Here we present a detailed method to perform VIGS in cassava, from target gene fragment to agroinoculation and VIGS quantitation