Near-Limb Zeeman and Hanle Diagnostics

"Weak" magnetic-field diagnostics in faint objects near the bright solar disk are discussed in terms of the level of non-object signatures, in particular, of the stray light in telescopes. Calculated dependencies of the stray light caused by diffraction at the 0.5-, 1.6-, and 4-meter entrance aperture are presented. The requirements for micro-roughness of refractive and reflective primary optics are compared. Several methods for reducing the stray light (the Lyot coronagraphic technique, multiple stages of apodizing in the focal and exit pupil planes, apodizing in the entrance aperture plane with a special mask), and reducing the random and systematic errors are noted. An acceptable level of stray light in telescopes is estimated for the V-profile recording with a signal-to-noise ratio greater than three. Prospects for the limb chromosphere magnetic measurements are indicated.Comment: 11 pages, 3 figure

Polymeric delivery systems for nucleic acid therapeutics: Approaching the clinic

Gene therapy using nucleic acids has many clinical applications for the treatment of diseases with a genetic origin as well as for the development of innovative vaccine formulations. Since nucleic acids in their free form are rapidly degraded by nucleases present in extracellular matrices, have poor pharmacokinetics and hardly pass cellular membranes, carrier systems are required. Suitable carriers that protect the nucleic acid payload against enzymatic attack, prolong circulation time after systemic administration and assist in cellular binding and internalization are needed to develop nucleic acid based drug products. Viral vectors have been investigated and are also clinically used as delivery vehicles. However, some major drawbacks are associated with their use. Therefore there has been substantial attention on the use of non-viral carrier systems based on cationic lipids and polymers. This review focuses on the properties of polymer-based nucleic acid formulations, also referred as polyplexes. Different polymeric systems are summarized, and the cellular barriers polyplexes encounter and ways to tackle these are discussed. Finally attention is given to the clinical status of non-viral nucleic acid formulations

Proapoptotic effect of a micropollutant (tris-(2-chloroethyl)-phosphate) at environmental level in primary cultured renal proximal tubule cells

10.2166/wh.2012.180Journal of Water and Health104522-53

Polymeric delivery systems for nucleic acid therapeutics: Approaching the clinic

Gene therapy using nucleic acids has many clinical applications for the treatment of diseases with a genetic origin as well as for the development of innovative vaccine formulations. Since nucleic acids in their free form are rapidly degraded by nucleases present in extracellular matrices, have poor pharmacokinetics and hardly pass cellular membranes, carrier systems are required. Suitable carriers that protect the nucleic acid payload against enzymatic attack, prolong circulation time after systemic administration and assist in cellular binding and internalization are needed to develop nucleic acid based drug products. Viral vectors have been investigated and are also clinically used as delivery vehicles. However, some major drawbacks are associated with their use. Therefore there has been substantial attention on the use of non-viral carrier systems based on cationic lipids and polymers. This review focuses on the properties of polymer-based nucleic acid formulations, also referred as polyplexes. Different polymeric systems are summarized, and the cellular barriers polyplexes encounter and ways to tackle these are discussed. Finally attention is given to the clinical status of non-viral nucleic acid formulations

Inclined lidar observations of boundary layer aerosol particles above the Kongsfjord, Svalbard

An inclined lidar with vertical resolution of 0.4 m was used for detailed boundary layer studies and to link observations at Zeppelin Mountain (474 m) and Ny-Ålesund, Svalbard. We report on the observation of aerosol layers directly above the Kongsfjord. On 29 April 2007, a layer of enhanced backscatter was observed in the lowest 25 m above the open water surface. The low depolarization ratio indicated spherical particles. In the afternoon, this layer disappeared. The ultrafine particle concentration at Zeppelin and Corbel station (close to the Kongsfjord) was low. On 1 May 2007, a drying process in the boundary layer was observed. In the morning, the atmosphere up to Zeppelin Mountain showed enhanced values of the backscatter coefficient. Around noon, the top of the highly reflecting boundary layer decreased from 350 to 250 m. The top of the boundary layer observed by lidar was confirmed by radiosonde data