Synthesis and Crystal Structure of Dipotassium Salts of N-Alkyl-N-{[O-alkoxy(hydroxy)phosphoryl]methyl}ditiocarbamic Acids

© 2020, Pleiades Publishing, Ltd. Abstract: A one-pot method for the synthesis of dipotassium salts of N-alkyl-N-{[O-alkoxy(hydroxy)phosphoryl]methyl}dithiocarbamic acids has been elaborated. Dipotassium salts of N-isopropyl-, N-butyl-, and N-cyclohexyl-N-{[hydroxy(O-ethoxy)phosphoryl]methyl}dithiocarbamic acids as well as N-{[O-butoxy(hydroxy)phosphoryl]methyl}-N-(2-methoxyethyl)dithiocarbamic acid have been synthesized and isolated. Crystal and molecular structure of the latter compound have been elucidated

Towards universal stimuli-responsive drug delivery systems: Pillar[5]arenes synthesis and self-assembly into nanocontainers with tetrazole polymers

In this work, we have proposed a novel universal stimulus-sensitive nanosized polymer system based on decasubstituted macrocyclic structures—pillar[5]arenes and tetrazole-containing polymers. Decasubstituted pillar[5]arenes containing a large, good leaving tosylate, and phthalimide groups were first synthesized and characterized. Pillar[5]arenes containing primary and tertiary amino groups, capable of interacting with tetrazole-containing polymers, were obtained with high yield by removing the tosylate and phthalimide protection. According to the fluorescence spectroscopy data, a dramatic fluorescence enhancement in the pillar[5]arene/fluorescein/polymer system was observed with decreasing pH from neutral (pH = 7) to acidic (pH = 5). This indicates the destruction of associates and the release of the dye at a pH close to 5. The presented results open a broad range of opportunities for the development of new universal stimulus-sensitive drug delivery systems containing macrocycles and nontoxic tetrazole-based polymers

2D monomolecular nanosheets based on thiacalixarene derivatives: Synthesis, solid state self-assembly and crystal polymorphism

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Synthetic organic 2D materials are attracting careful attention of researchers due to their excellent functionality in various applications, including storage batteries, catalysis, thermoelectricity, advanced electronics, superconductors, optoelectronics, etc. In this work, thiacalix[4]arene derivatives functionalized by geranyl fragments at the lower rim in cone and 1,3-alternate conformations, that are capable of controlled self-assembly in a 2D nanostructures were synthesized. X-ray diffraction analysis showed the formation of 2D monomolecular-layer nanosheets from synthesized thiacalix[4]arenes, the distance between which depends on the stereoisomer used. It was established by DSC, FSC, and PXRD methods that the obtained macrocycles are capable of forming different crystalline polymorphs, moreover dimethyl sulphoxide (DMSO) is contributing to the formation of a more stable polymorph for cone stereoisomer. The obtained crystalline 2D materials based on synthesized thiacalix[4]arenes can find application in material science and medicine for the development of modern pharmaceuticals and new generation materials

Influence of recombinant codon‐optimized plasmid dna encoding vegf and fgf2 on co‐induction of angiogenesis

Several methods for the stimulation of skin wound repair have been proposed over the last few decades. The most promising among them are gene and stem cell therapy. Our present experiments combined several approaches via the application of human umbilical cord blood mononuclear cells (hUCB‐MC) that were transfected with pBud‐VEGF165‐FGF2 plasmid (gene-cell therapy) and direct gene therapy using pBud‐VEGF165‐FGF2 plasmid to enhance healing of full thickness skin wounds in rats. The dual expression cassette plasmid pBud‐VEGF165‐FGF2 en-codes both VEGF and FGF2 therapeutic genes, expressing pro‐angiogenic growth factors. Our results showed that, with two weeks post‐transplantation, some transplanted cells still retained expression of the stem cell and hematopoietic markers C‐kit and CD34. Other transplanted cells were found among keratinocytes, hair follicle cells, endothelial cells, and in the derma. PCNA expression studies revealed that transplantation of transfected cells terminated proliferative processes in regenerating wounds earlier than transplantation of untransfected cells. In the direct gene therapy group, four days post‐operatively, the processes of flap revascularization, while using Easy LDI Microcirculation Camera, was higher than in control wounded skin. We concluded that hUCB‐ MC can be used for the treatment of skin wounds and transfection these cells with VEGF and FGF2 genes enhances their regenerative abilities. We also concluded that the application of pBud‐ VEGF165‐FGF2 plasmids is efficient for the direct gene therapy of skin wounds by stimulation of wound revascularization