Antiseptics on the basis of terpenoid compounds: synthesis, properties and application

Currently, chemical methods of pest bio deterioration of materials, products and constructions are the main complex of destructive actions. In particularwood preservative very effective way to save forest resources for the longest time protects. In developed countries, the use of antiseptics reached industrial scale. The search of effective forms of applyingantiseptics and rational use of them continue to be relevant tasks. The review is devoted to the methods of antiseptics on the basis of the terpenoid compounds. It is given broad spectrum biocide properties of antiseptics and use of anti-septic funds in protective compositions and coatings for various purposes

РАЗВИТИЕ КОНТРАСТНЫХ ПРЕПАРАТОВ ДЛЯ ДИАГНОСТИКИ ВНУТРИПРОТОКОВЫХ ЗАБОЛЕВАНИЙ МОЛОЧНОЙ ЖЕЛЕЗЫ

Breast is the organ with low natural visibility, this fact don’t let us to produce an image of milk ducts on the plain film. Assessment of the milk ducts is an important condition to identify the earliest signs of diseases like cancer and benign nature. It is known that up to 80% of the cancer develops ductal epithelium of the breast. Traditional ultrasonic scanning because of it’s inefficient resolution capability and physical peculiarities of method enables us to define only 4-5 mm intraductal tumors. Many attempts of improvement of intraductal breast deceases diagnostic were made for many years to come. Different technologies: cytological analysis, radiography, US, MRI were used for achieving these purposes. They didn’t always allow us to think definitely of one ore other process. It led to a need to improve the objective methods of visual diagnostics due to increasing contrast of objects being investigated including radiography.Молочная железа - орган, обладающий низкой естественной контрастностью, что ограничивает возможности получения изображения млечных протоков на обзорном рентгеновском снимке. Оценка состояния млечных протоков - важное условие для выявления самых ранних признаков заболеваний как злокачественной, так и доброкачественной природы. Известно, что до 80% случаев рака развивается из эпителия протоков молочной железы. Традиционное УЗИ в силу недостаточной разрешающей способности и физических особенностей метода позволяет увидеть протоковые разрастания размерами лишь от 4-5 мм. В течение многих лет предпринимались попытки улучшения диагностики внутрипротоковых заболеваний молочной железы. Для этих целей использовались различные технологии: цитологическое исследование, рентгенография, УЗИ, МРТ. Они далеко не всегда позволяли достоверно судить о том или ином процессе. Это привело к необходимости усовершенствования объективных методов визуальной диагностики за счет повышения контрастности изучаемого объекта, в том числе при рентгенографии

Evaluation of the efficiency of phytotherapy with mastodinone and gelarium in patiens withdiffuse forms of mastopathies

The authors define mastopathies and consider the major forms of this disease. They also present the results of the clinical trials evaluating the efficiency of the herbal drugs mastodinone and gelatium in different forms of diffuse mastopathies, accompanied by the pain syndrome of varying degree, and depression

Cryopreservation of epididymal semen of domestic cat

Domestic cat (Felis silvestris catus) is used as a model species for developing effective methods of wild felids’ semen cryopreservation. The present study represents a comparison of domestic cat semen cryopreservation with two commercially available cryoprotectant agents (CPAs): CaniPlus Freeze (CPF) and SpermFreeze (SF). Semen was collected from the caudal epididymises of adult males and frozen with CPF and SF, correspondingly. The viability of frozen-thawed spermatozoa was evaluated by VitalScreen kit, staining with hematoxylin and eosin was performed for analysis of the spermatozoa morphology; both analyses were combined with the light microscopy. The viability rate of the frozenthawed semen cryopreserved with CPF and SF did not differ: 32.3±4.4 % for CPF and 43.3±4.0 % for SF. Total percentage of morphologically normal spermatozoa after freezing and thawing domestic cat semen was 26.0±2.3 % for CPF and 23.9±1.9 % for SF. In both cases, there were no differences from non-frozen semen, in the latter group the total percentage of spermatozoa with normal morphology was 29.0±4.1 %. The most frequent anomalies were the anomalies of tail, and the rarest anomalies were head defects. The percentages of spermatozoa with anomalies of the head, mid piece, tail and combined did not differ in these three groups. Taken together these results suggest that both CPAs are suitable for the purpose of domestic cat semen freezing and cryopreservation, although CPF was designed for Canidae semen cryopreservation and SF was developed for human semen freezing and so far was used exclusively in reproductive medicine. It might be concluded that these two commercially available cryoprotectant media are applicable for the purposes of domestic cat breeds’ semen cryopreservation

MOLECULAR MARKING OF SUNFLOWER LINES WITH DIFFERENT ABILITY TO SUPPRESSION OF THE CYTOPLASMIC MALE STERILITY PHENOTYPE

Ninety five lines of sunflower genetic collection differing by their ability to suppress the CMS phenotype were molecularly marked with the use of 7 primer pairs. Using the STS marker orfH522, a sterile (PET1) cytoplasmon was identified in 79 lines, which confirmed indirectly the presence of fertility restoration genes in their genotypes. The majority of these lines also have a complex of molecular markers linked to the Rf1 gene. The HRG01, HRG02 and STS115 markers showed the best diagnostic value in revealing the Rf1 gene in the examined material. The data on allelic variation of the microsatellite loci ORS224, ORS511 and ORS799 were obtained for the first time

Comparison of in vivo and in vitro preimplantation embryo development in OXYS and WAG rats

OXYS rats are the model of precocious senescence. Numerous studies addressed physiology and behavior in rats of this strain during a postnatal period of their life, however, preimplantation development in OXYS rats has not yet been investigated. This study is addressing preimplantation embryonic development in OXYS rats both in vivo and in vitro. Rats of the WAG strain were used as controls. For studying the in vivo development, the embryos were collected from OXYS and WAG rats on day 5 post coitum, the stages of embryo development were estimated, the percentage of embryos at blastocyst stage and the cell numbers in these blastocysts were counted. In a special experiment, for studying in vitro development, the embryos were collected from both rat strains on day 4 post coitum and were cultured in vitro in P1 medium for 48 hours with or without supplementation with IGF-1 (200 ng/mL). Thereafter the percentage of embryos at blastocyst stage and the cell numbers in these blastocysts were counted in the same manner as for the in vivo experiment. This study reports that in vivo derived blastocysts of OXYS rats contain fewer cells on day 5 of their development than in vivo derived blastocysts of WAG rats. In vitro culture of the early preimplantation embryos in P1 medium mitigated the difference in the rate of embryo development between these two strains, the addition of IGF-1 into culture medium exerts neither negative nor positive effect on the rate of in vitro embryo development in rats of both strains

A comparison of different cryoprotectant solutions and thawing methods for cryo­preservation of embryos of mice and rats

The proper choice of cryoprotectant and thawing method affects cryopreservation efficiency. A freezing-thawing method for sparing embryonic cells was evaluated in experiments with ICR mice. Cleavage-stage embryos of ICR mice, GC rats, and OXYS rats were collected on Day 3 of pregnancy and frozen in plastic straws according to a standard protocol. Permeating (ethylene glycol and glycerol) and nonpermeating (sucrose) cryoprotectants and their combinations were compared during the freezing of ICR mouse embryos. With these mice, two thawing methods were compared: rapid (water bath, 10 s, 37 °С) and slow (40 s, room temperature; 40 s, 30 °С). Embryo viability in mice and rats was evaluated by their in vitro culturing after thawing. Our data on mice indicate that slow thawing is more suitable for sparing the integrity of embryonic cells; moreover, supplementation of the main cryoprotectant (either ethylene glycol or glycerol) with sucrose is beneficial for subsequent in vitro culture, especially in the case of glycerol. This freezing-thawing protocol (with glycerol and sucrose as cryoprotectant agents and slow thawing) was applied to rats of the GC and OXYS strains; the survival rate after cryopreservation was 68–83.3 %, and the rate of in vitro development was 64.7–66.6 %