36 research outputs found
Isolation of Substances That Stimulate RNA Synthesis from Red Kidney Bean: Their Activities in Lymphocytes and Escherichia coli
No full textEffect of Diethyl Pyrocarbonate on the Biological Activity of Deoxyribonucleic Acids Isolated from Bacteriophages
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A New Method Based on the Use of Diethyl Pyrocarbonate as a Nuclease Inhibitor for the Extraction of Undegraded Nucleic Acid from Plant Tissues
No full textEffect of Diethylpyrocarbonate on the Antiviral and Interferon-Inducing Activities of Viral and Nonviral Agents
No full textOn the Extraction of Ribonucleic Acid with Template Activity from Barley Embryos Using Diethyl Pyrocarbonate as Nuclease Inhibitor
No full textViral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
No full textHigh-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing
