Concentration dependence of the effect of bacillus intermedium ribonuclease on the yeast saccharomyces cerevisiae

-Bacillus intermedius RNase added at a low concentration (0.001 |ig/ml) stimulated yeast growth, while a high RNase concentration (1500 Jig/ml) was inhibitory to yeast growth. The inhibitory effect of RNase was transient and correlated with the increase in the trehalose pool of yeast cells. The number of unbudded cells in the yeast population tended to decrease under the action of low concentrations of bacillar RNase and to increase under the action of high concentrations of this enzyme

Effects of Bacillus intermedius ribonuclease on the properties of Saccharomyces cerevisiae

Effects of Bacillus intermedius ribonuclease on the physiological, biochemical, and consumer properties of baker's yeast Saccharomyces cerevisiae were studied. This enzyme improved the yeast raising strength and increased the cell tolerance to various adverse factors. The antistress effect of RNase correlated with an earlier start of the stationary growth phase and increased trehalose pool

Concentration dependence of the effect of Bacillus intermedius ribonuclease on the yeast Saccharomvces cerevisiae

Bacillus intermedius RNase added at a low concentration (0.001 μg/ml) stimulated yeast growth, while a high RNase concentration (1500 μg/ml) was inhibitory to yeast growth. The inhibitory effect of RNase was transient and correlated with the increase in the trehalose pool of yeast cells. The number of unbudded cells in the yeast population tended to decrease under the action of low concentrations of bacillar RNase and to increase under the action of high concentrations of this enzyme. © 2000 MAIK "Nauka/Interperiodica"

Effects of Bacillus intermedius ribonuclease on properties of Saccharomyces cerevisiae

Effects of Bacillus intermedius ribonuclease on physiological, biochemical, and consumer properties of baker's yeast Saccharomyces cerevisiae were studied. This enzyme improved the yeast raising strength and increased the cell tolerance to various adverse factors. The antistress effect of RNase correlated with an earlier start of the stationary growth phase and increased trehalose pool

Effects of RNase from Bacillus intermedius on Growth of Saccharomyces cerevisiae

The effects of RNase from Bacillus intermedius on proliferation of Saccharomyces cerevisiae were studied. The enzyme (0.01 μg/ml) stimulated the yeast cell budding. This effect was dose-dependent and required an appropriate physiological stage of the growing culture cells. RNase produced maximal effects when added to exponentially growing cultures, Analysis of the age structure of the population showed that exogenous RNase stimulated the cell cycle at a stage preceding the initiation of DNA synthesis and budding of single yeast cells and cells occurring at the budding stage III. RNase did not decrease the buoyancy and osmotic sensitivity of baker's yeast

Effect of Bacillus intermedius ribonucleases with different catalytic activities on the growth of Escherichia coli and Bacillus subtilis

The effect of the Bacillus intermedius ribonuclease and its mutant forms derived by site-specific mutagenesis on the growth of gram-positive and gram-negative bacteria was studied. Both catalytically active and catalytically inactive (mutant) ribonucleases stimulated bacterial growth, the extent of stimulation correlating with the catalytic activity of the enzymes. It was suggested that the biological activity of exogenous ribonucleases is mainly due to their catalytic activity. © 1998 MAHK Hayka/Interperiodica Publishing

Effect of bacillus intermedius ribonucleases with different catalytic activities on the growth of escherichia coli and bacillus subtilis

The effect of the Bacillus intermedius ribonuclease and its mutant forms derived by site-specific mutagenesis on the growth of gram-positive and gram-negative bacteria was studied. Both catalytically active and catalytically inactive (mutant) ribonucleases stimulated bacterial growth, the extent of stimulation correlating with the catalytic activity of the enzymes. It was suggested that the biological activity of exogenous ribonucleases is mainly due to their catalytic activity

Effect of the RNase from bacillus intermedium on growth and physiological characteristics of escherichia coli

The effect of the RNase from Bacillus intermedium on the growth of Escherichia coli was investigated. RNase added to growth medium enhanced the synthesis of DNA, RNA, and protein and stimulated cell division; the degree of stimulation depended on the enzyme concentration. A necessary condition for stimulation was the adsorption of the enzyme on the cell surface and its interaction with the cytoplasmic membrane, as demonstrated immunocytochemically. The adsorption of the enzyme was accompanied by a 43% decrease in the surface charge density. Other effects of RNase involved a 25% increase in the growth rate, a 38% biomass gain, and generation time shortening by 10 min. The stimulation of bacterial growth correlated with the stimulation of cellular respiration rate

Effects of RNase from Bacillus intermedius on the growth of Saccharomyces cerevisiae

The effects of RNase from Bacillus intermedius on the proliferation of Saccharomyces cerevisiae were studied. The enzyme (0.01 μg/ml) stimulated the yeast cell budding. This effect was dose-dependent and required an appropriate physiological state of the growing culture cells. RNase produced maximal effects when added to exponentially growing cultures. Analysis of the age structure of the population showed that exogenous RNase stimulated the cell cycle at a state preceding the initiation of DNA synthesis and budding of single yeast cells or cells occurring at the budding stage III. RNase did not decrease the buoyancy and osmotic sensitivity of baker's yeast

Effect of the RNase from bacillus intermedius on growth and physiological characteristics of Escherichia coli

The effect of the RNase from Bacillus intermedius on the growth of Escherichia coli was investigated. RNase added to growth medium enhanced the synthesis of DNA, RNA, and protein and stimulated cell division; the degree of stimulation depended on the enzyme concentration. A necessary condition for stimulation was the adsorption of the enzyme on the cell surface and its interaction with the cytoplasmic membrane, as demonstrated immunocytochemically. The adsorption of the enzyme was accompanied by a 43% decrease in the surface charge density. Other effects of RNase involved a 25% increase in the growth rate, a 38% biomass gain, and generation time shortening by 10 min. The stimulation of bacterial growth correlated with the stimulation of the cellular respiration rate. © 1999 MAHK "Hayka/Interperiodica"