41 research outputs found
Role of foreign language teacher shaping studentsβ research skills
Β© 2015 Canadian Center of Science and Education. All rights reserved. Nowadays many foreign language teachers are not enough aware about the significance of the research component within their profile discipline, arguing that students even in their native language do not have enough use of fundamentals in their scientific professional activities. Therefore, this article is aimed to study the role of foreign language teacher when mastering studentsβ research skills in the process of learning English as a foreign language. The study results have confirmed that process of mastering studentsβ research skills when learning foreign language is directly connected with their teacherβs own research skill level. These article materials have practical value both for foreign language teachers and for students enrolled for foreign languages programs of education sciences faculties at high schools
Pedagogical potential of the career guidance course βProfessional career planningβ to form pupils and studentsβ self-determination in the integrated system βschoolβvocational collegeβ
Β© 2015, Canadian Center of Science and Education. All rights reserved. The article is aimed at revealing pedagogical potential of an elective career guidance course to form pupils and studentsβ professional self-determination in the integrated system βschoolβvocational collegeβ. The basic approach to research this problem is an integrative one that causes efficiency of pupils and studentsβ career guidance work in dual integrated system. The content of career guidance course βProfessional Career Planningβ developed and presented in this article is aimed at pupilsβ professional self-determination formation, consciousness and steady interest in choosing future profession. As for vocational college students, the course is directed towards the studentsβ choice of their own individual educational and career way, revealing their adaptation abilities to future professional activity. Materials of the article are of value for school and vocational college teachers while organizing and planning career guidance work at the educational institutions
Features of formation of students foreign language competence in self-learning activity
Β© Canadian Center of Science and Education. This article is aimed to identify characteristics of the formation of students foreign language skills in the process of self-learning activity. It is proved that foreign language competences are formed as a complex of integrated steady students education as subjects of self-learning activity including: language, speech, compensatory, educational and informative, socio-cultural, communicative and projecting competences. The article submissions are of practical value to foreign language teachers in the organization and planning of students educational and self-learning activity aimed to establish and improve their foreign language competence
Designing technology of English language teaching content based on international component
Β© 2015, Canadian Center of Science and Education. All rights reserved. This paper aims to develop designing technology of English language teaching content for students at higher education institution in the context of education internationalization. It highlighted the analytical, pre-designing, designing and correction-implementation stages as well as their successive phases to achieve a specific goal, i.e. English language teaching content designing in terms of international component. The materials of this paper are of practical value for teachers of foreign languages in the selecting and structuring the language teaching content in the context of education internationalization
ΠΠΠΠ―ΠΠΠ ΠΠΠΠΠ‘ΠΠΠΠΠ¬ΠΠΠ Π€ΠΠ ΠΠ« Π‘Π’Π ΠΠΠ’ΠΠΠΠΠΠΠ« ΠΠ ΠΠΠ ΠΠΠΠΠΠΠΠ Π-ΠΠΠΠΠ ΠΠ
Liposomal streptokinase (a mixture of free (66.3Β %) and entrapped (23.7Β %) drug) with a diameter of ~60 nm was prepared. The analysis of the D-dimers formation kinetics in the dogβs blood plasma showed that the liposomal streptokinase hada prolonged effect up to 180 minutes.ΠΠΎΠ»ΡΡΠ΅Π½Ρ Π»ΠΈΠΏΠΎΡΠΎΠΌΡ ΡΠΎ ΡΡΡΠ΅ΠΏΡΠΎΠΊΠΈΠ½Π°Π·ΠΎΠΉ Ρ ΡΠ°Π·ΠΌΠ΅ΡΠ°ΠΌΠΈ ~60 Π½ΠΌ, ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡΠΈΠ΅ ΡΠΎΠ±ΠΎΠΉ ΡΠΌΠ΅ΡΡ, ΡΠΎΡΡΠΎΡΡΡΡ ΠΈΠ· ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΠΎΠ³ΠΎ (66,3Β %) ΠΈ ΡΠ²ΡΠ·Π°Π½Π½ΠΎΠ³ΠΎ (23,7Β %) ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°. ΠΠ° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ Π°Π½Π°Π»ΠΈΠ·Π° ΠΊΠΈΠ½Π΅ΡΠΈΠΊΠΈ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π-Π΄ΠΈΠΌΠ΅ΡΠΎΠ² Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΡΠΎΠ±Π°ΠΊ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π»ΠΈΠΏΠΎΡΠΎΠΌΠ°Π»ΡΠ½Π°Ρ ΡΠΎΡΠΌΠ° ΡΡΡΠ΅ΠΏΡΠΎΠΊΠΈΠ½Π°Π·Ρ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ ΠΏΡΠΎΠ»ΠΎΠ½Π³ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ ΡΡΡΠ΅ΠΊΡΠΎΠΌ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 180 ΠΌΠΈΠ½
ΠΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈ ΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π½Π½ΠΎΡΡΠΈ Π·Π΅ΡΠ½Π° Ρ Π³Π΅Π½ΠΎΡΠΈΠΏΠΎΠ² ΠΏΡΠ΅Π½ΠΈΡΡ Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ Π°Π»Π»Π΅Π»ΡΠΌΠΈ Π³Π΅Π½Π° NAM-Π1
The identification of a functional NAM-B1 allele associated with a high content of grain protein and essential microelements in wheat relatives increased the distant hybridization significance for bread wheat nutritional value. The allelic polymorphism of the NAM-B1 gene in 22 wheat lines with a genetic material of T. dicoccoides, T. dicoccum, T. spelta, T. kiharΠ°e and their parental forms and the effects of NAM-B1 gene allelic variations on the content of grain protein and essential microelements and productivity traits (vegetation period 2017β2021) were evaluated. The functional NAM-B1 allele was identified only in the samples of wheat relatives among the parental forms. All parental varieties and most of introgressive lines (77.3 %) had a non-functional allele. The genotypes with the functional NAM-B1 allele were characterized by a higher plant height and tillering, but by lower spike productivity compared to the non-functional allele genotypes. The presence of the functional NAM-B1 allele provided a high level of grain protein and zinc content and never decreased significantly a thousand-kernel weight across all studied environments. The functional NAM-B1 allele introgression could be a resource for improving the grain wheat nutritional value.ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΠ΅ Ρ ΡΠΎΡΠΎΠ΄ΠΈΡΠ΅ΠΉ ΠΏΡΠ΅Π½ΠΈΡΡ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ (Π΄ΠΈΠΊΠΎΠ³ΠΎ) Π°Π»Π»Π΅Π»Ρ Π³Π΅Π½Π° NAM-Π1, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Ρ Π²ΡΡΠΎΠΊΠΈΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ Π±Π΅Π»ΠΊΠ° ΠΈ ΠΊΠ»ΡΡΠ΅Π²ΡΡ
ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² Π·Π΅ΡΠ½Π΅, ΡΠ²Π΅Π»ΠΈΡΠΈΠ»ΠΎ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ ΠΎΡΠ΄Π°Π»Π΅Π½Π½ΠΎΠΉ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ Π΄Π»Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π½Π½ΠΎΡΡΠΈ Π·Π΅ΡΠ½Π° T. aestivum L. ΠΠ·ΡΡΠ΅Π½ Π°Π»Π»Π΅Π»ΡΠ½ΡΠΉ ΡΠΎΡΡΠ°Π² Π³Π΅Π½Π° NAM-B1 Ρ 22 Π»ΠΈΠ½ΠΈΠΉ ΠΌΡΠ³ΠΊΠΎΠΉ ΠΏΡΠ΅Π½ΠΈΡΡ Ρ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ T. dicoccoides, T. dicoccum, T. spelta, T. kiharΠ°e ΠΈ ΠΈΡ
ΡΠΎΠ΄ΠΈΡΠ΅Π»ΡΡΠΊΠΈΡ
ΡΠΎΡΠΌ ΠΈ ΠΎΡΠ΅Π½Π΅Π½ ΡΡΡΠ΅ΠΊΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π°Π»Π»Π΅Π»Π΅ΠΉ Π³Π΅Π½Π° NAM-B1 Π½Π° ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠ°, ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² Π·Π΅ΡΠ½Π΅ ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ (Π²Π΅Π³Π΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠ΅ ΠΏΠ΅ΡΠΈΠΎΠ΄Ρ 2017β2021 Π³Π³.). Π‘ΡΠ΅Π΄ΠΈ ΡΠΎΠ΄ΠΈΡΠ΅Π»ΡΡΠΊΠΈΡ
ΡΠΎΡΠΌ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π°Π»Π»Π΅Π»Ρ Π³Π΅Π½Π° NAM-B1 ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ ΡΠΎΠ»ΡΠΊΠΎ Ρ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π²ΠΈΠ΄ΠΎΠ²-ΡΠΎΡΠΎΠ΄ΠΈΡΠ΅ΠΉ. ΠΡΠ΅ ΡΠΎΠ΄ΠΈΡΠ΅Π»ΡΡΠΊΠΈΠ΅ ΡΠΎΡΡΠ° ΠΈ Π±ΠΎΠ»ΡΡΠ°Ρ ΡΠ°ΡΡΡ ΠΈΠ½ΡΡΠΎΠ³ΡΠ΅ΡΡΠΈΠ²Π½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ (77,3 %) ΠΈΠΌΠ΅Π»ΠΈ ΠΌΡΡΠ°Π½ΡΠ½ΡΠΉ (Π½Π΅ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ) Π°Π»Π»Π΅Π»Ρ. ΠΠ½Π°Π»ΠΈΠ· ΡΡΠ΅Π΄Π½ΠΈΡ
ΠΌΠ½ΠΎΠ³ΠΎΠ»Π΅ΡΠ½ΠΈΡ
Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² Π²ΡΡΠ²ΠΈΠ», ΡΡΠΎ Π³Π΅Π½ΠΎΡΠΈΠΏΡ Ρ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌ Π°Π»Π»Π΅Π»Π΅ΠΌ NAM-Π1 Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π»ΠΈΡΡ Π±ΠΎΠ»ΡΡΠ΅ΠΉ Π²ΡΡΠΎΡΠΎΠΉ ΡΠ°ΡΡΠ΅Π½ΠΈΡ ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΊΡΡΡΠΈΡΡΠΎΡΡΡΡ, Π½ΠΎ Π±ΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΠΎΠ»ΠΎΡΠ° ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π³Π΅Π½ΠΎΡΠΈΠΏΠ°ΠΌΠΈ, Π½Π΅ΡΡΡΠΈΠΌΠΈ Π½Π΅ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ Π°Π»Π»Π΅Π»Ρ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π½Π°Π»ΠΈΡΠΈΠ΅ Π°Π»Π»Π΅Π»Ρ Π΄ΠΈΠΊΠΎΠ³ΠΎ ΡΠΈΠΏΠ° ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°Π΅Ρ Π²ΡΡΠΎΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ Π±Π΅Π»ΠΊΠ° ΠΈ ΡΠΈΠ½ΠΊΠ° Π² Π·Π΅ΡΠ½Π΅ Π½Π΅Π·Π°Π²ΠΈΡΠΈΠΌΠΎ ΠΎΡ ΠΏΠΎΠ³ΠΎΠ΄Π½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΠΉ, ΠΈ ΠΏΡΠΈ ΡΡΠΎΠΌ Π½Π΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΌΡ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΌΠ°ΡΡΡ 1000 Π·Π΅ΡΠ΅Π½. ΠΠΎΠΊΠ°Π·Π°Π½Π° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈΠ½ΡΡΠΎΠ³ΡΠ΅ΡΡΠΈΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π°Π»Π»Π΅Π»Ρ NAM-Π1 ΠΎΡ Π²ΠΈΠ΄ΠΎΠ²-ΡΠΎΡΠΎΠ΄ΠΈΡΠ΅ΠΉ Π΄Π»Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π½Π½ΠΎΡΡΠΈ Π·Π΅ΡΠ½Π° ΠΌΡΠ³ΠΊΠΎΠΉ ΠΏΡΠ΅Π½ΠΈΡΡ
Π€ΠΠ ΠΠΠΠ’ΠΠ’ΠΠΠΠΠ― ΠΠΠ’ΠΠΠΠΠ‘Π’Π¬ ΠΠΠΠ’ΠΠ ΠΠ Π ΠΠΠ ARTHROBACTER Π ΠΠ₯ ΠΠΠΠΠ’ΠΠΠΠ§ΠΠ‘ΠΠΠ― ΠΠΠΠΠ’ΠΠ€ΠΠΠΠ¦ΠΠ―
Two-stage screening enabled one to define the composition of enzyme complexes produced by bacteria of genus ArthrobacterΒ in media with specific substrates and activity of individual constituents involved in the t ransformation of milk proteins,Β lipids and carbohydrates. It was found that enzyme complexes of Arthrobacter strains BIM B-2239, BIM B-2240, BIMΒ B-2241 and BIM B-2242 showed the most balanced activity of lipase, protease, Ξ²-galactosidase and glucose(xylose)isomeraseΒ components. Following the nucleotide sequence analysis of 16S rRNA gene the examined cultures were identified as ArthrobacterΒ sulfonivorans. 16S rRNA gene sequences over 1400 bp in size were deposited in GenBank database.Detailed investigation of conditions favoring the efficient hydrolysis of proteins and lipids by proteases and lipases producedΒ by Arthrobacter sulfonivorans strains BIM B-2239, BIM B-2240, BIM B-2241, BIM B-2242 and the transformation ofΒ milk lactose and derived hydrolysis products mediated by Ξ²-galactosidases and glucose(xylose)isomerases will allow one toΒ select microbial strain to develop the biotechnology of manufacturing hypoallergenic feed additive with prebiotic activityΒ from dairy substrates.Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π΄Π²ΡΡ
ΡΡΡΠΏΠ΅Π½ΡΠ°ΡΠΎΠ³ΠΎ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠΎΡΡΠ°Π² ΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ², ΠΏΡΠΎΠ΄ΡΡΠΈΡΡΠ΅ΠΌΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΡΠΌΠΈ ΡΠΎΠ΄Π° Arthrobacter Π² ΡΡΠ΅Π΄Π°Ρ
Ρ ΠΎ Ρ ΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΡΠ±ΡΡΡΠ°ΡΠΎΠΌ, ΠΈ Π° ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΈ Ρ
ΠΎ ΡΠ΄Π΅Π»ΡΠ½ΡΡ
ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ
Β Π² ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ², ΠΆΠΈΡΠΎΠ² ΠΈ ΡΠ³Π»Π΅Π²ΠΎΠ΄ΠΎΠ² ΠΌΠΎΠ»ΠΎΠΊΠ°. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ±Π°Π»Π°Π½ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΠΏΠΎ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΎΠ΄Π½ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎ Π»ΠΈΠΏΠ°Π·, ΠΏΡΠΎΡΠ΅Π°Π·, Ξ²-Π³Π°Π»Π°ΠΊΡΠΎΠ·ΠΈΠ΄Π°Π· ΠΈ Π³Π»ΡΠΊΠΎΠ·ΠΎ(ΠΊΡΠΈΠ»ΠΎΠ·ΠΎ)ΠΈΠ·ΠΎΠΌΠ΅ΡΠ°Π· ΡΠ²Π»ΡΡΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΡΠ΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ ΡΡΠ°ΠΌΠΌΠΎΠ²Β ΠΠΠ Π-2239, ΠΠΠ Π-2240, ΠΠΠ Π-2241 ΠΈ ΠΠΠ Π-2242 Arthrobacter sp.ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π° Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π³Π΅Π½ΠΎΠ² 16S ΡΠ ΠΠ ΡΠΊΠ°Π·Π°Π½Π½ΡΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΠΊΡΠ»ΡΡΡΡΡ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Ρ ΠΊΠ°ΠΊ Arthrobacter sulfonivorans. ΠΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΈΡ
Π³Π΅Π½ΠΎΠ² 16S ΡΠ ΠΠ ΠΏΡΠΎΡΡΠΆΠ΅Π½Π½ΠΎΡΡΡΡ Π±ΠΎΠ»Π΅Π΅ 1400 ΠΏ.Β ΠΎ. Π΄Π΅ΠΏΠΎΠ½ΠΈΡΠΎΠ²Π°Π½Ρ Π² Π±Π°Π·Π΅ Π΄Π°Π½Π½ΡΡ
GenBank.ΠΠ΅ΡΠ°Π»ΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ»ΠΎΠ²ΠΈΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π° Π±Π΅Π»ΠΊΠΎΠ² ΠΈ ΠΆΠΈΡΠΎΠ² Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ ΠΏΡΠΎΠ΄ΡΡΠΈΡΡΠ΅ΠΌΡΡ
ΡΡΠ°ΠΌΠΌΠ°ΠΌΠΈΒ ΠΠΠ Π-2239, ΠΠΠ Π-2240, ΠΠΠ Π-2241 ΠΈ ΠΠΠ Π-2242 Arthrobacter sp. Π»ΠΈΠΏΠ°Π· ΠΈ ΠΏΡΠΎΡΠ΅Π°Π·, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΠΈ Π»Π°ΠΊΡΠΎΠ·ΡΒ ΠΌΠΎΠ»ΠΎΠΊΠ° ΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² Π΅Π΅ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π° Ρ ΡΡΠ°ΡΡΠΈΠ΅ΠΌ Ξ²-Π³Π°Π»Π°ΠΊΡΠΎΠ·ΠΈΠ΄Π°Π· ΠΈ Π³Π»ΡΠΊΠΎΠ·ΠΎ(ΠΊΡΠΈΠ»ΠΎΠ·ΠΎ)ΠΈΠ·ΠΎΠΌΠ΅ΡΠ°Π· ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ ΠΎΡΠΎΠ±ΡΠ°ΡΡ ΡΡΠ°ΠΌΠΌ-ΠΏΡΠΎΠ΄ΡΡΠ΅Π½Ρ Π΄Π»Ρ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π±ΠΈΠΎΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΈΠ· ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΡΡΡ Π³ΠΈΠΏΠΎΠ°Π»Π»Π΅ΡΠ³Π΅Π½Π½ΠΎΠΉ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΊΠΎΡΠΌΠΎΠ²ΠΎΠΉ Π΄ΠΎΠ±Π°Π²ΠΊΠΈ ΠΏΡΠ΅Π±ΠΈΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ
ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅, Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΠΌΠ° Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ Paenibacillus species
Bacterial variant PS-K-17 was isolated from wheat grain contaminated by polysaccharide-producing microbiota for further characterization. It was found that the isolate grown on agar slants and in submerged culture on media with specific substrates synthesized beta-galactosidase, amylase, protease, pectinase, cellulase, beta-glucanase, lipase (esterase), alginase, extracellular polysaccharides, and pigments, probably carotenoids. Based on cultural-morphological and physiological-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene (access code MF443394 in GenBank) the bacterial culture was identified as Paenibacillus species PS-K-17. The studied isolate forms one phylogenetic branch with type strains Paenibacillus nicotianae (98.3 %), Paenibacillus hordei (98.2 %), Paenibacillus kyungheensis (97.9 %), holding wherein a separate position. Strain Paenibacillus sp. PS-K-17 may find use in biotechnology as a producer of extracellular polysaccharides and enzymes splitting plant polymeric substances as well as a component of microbial consortium-ingredient of a new complex feed additive.ΠΠ· Π·Π΅ΡΠ½Π° ΠΏΡΠ΅Π½ΠΈΡΡ, ΠΊΠΎΠ½ΡΠ°ΠΌΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΠ°Π·ΡΡΡΠ΅ΠΉ ΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΠΎΠΉ, Π²ΡΠ΄Π΅Π»Π΅Π½Π° ΠΈ ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Π° ΠΊΡΠ»ΡΡΡΡΠ° Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΠ‘-Π-17. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΈΠ·ΠΎΠ»ΡΡ Π½Π° Π°Π³Π°ΡΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΡ
ΡΡΠ΅Π΄Π°Ρ
ΠΈ Π² Π³Π»ΡΠ±ΠΈΠ½Π½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠ΅ ΡΠΎ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΡΠ±ΡΡΡΠ°ΡΠ°ΠΌΠΈ ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΡΠ΅Ρ Π±Π΅ΡΠ°-Π³Π°Π»Π°ΠΊΡΠΎΠ·ΠΈΠ΄Π°Π·Ρ, Π°ΠΌΠΈΠ»Π°Π·Ρ, ΠΏΡΠΎΡΠ΅Π°Π·Ρ, ΠΏΠ΅ΠΊΡΠΈΠ½Π°Π·Ρ, ΡΠ΅Π»Π»ΡΠ»Π°Π·Ρ, Π±Π΅ΡΠ°- Π³Π»ΡΠΊΠ°Π½Π°Π·Ρ, Π»ΠΈΠΏΠ°Π·Ρ (ΡΡΡΠ΅ΡΠ°Π·Ρ), Π°Π»ΡΠ³ΠΈΠ½Π°Π·Ρ, Π° ΡΠ°ΠΊΠΆΠ΅ Π²Π½Π΅ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ ΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄Ρ ΠΈ ΠΏΠΈΠ³ΠΌΠ΅Π½ΡΡ, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ, ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄Ρ. ΠΠ° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎ-ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΎ-Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠΈΠ»ΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ Π³Π΅Π½Π° 16S ΡΠ ΠΠ (ΠΊΠΎΠ΄ Π΄ΠΎΡΡΡΠΏΠ° MF443394 Π² GenBank) Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½Π°Ρ ΠΊΡΠ»ΡΡΡΡΠ° ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π° ΠΊΠ°ΠΊ Paenibacillus species ΠΠ‘-Π-17. ΠΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΠΉ ΠΈΠ·ΠΎΠ»ΡΡ ΠΎΠ±ΡΠ°Π·ΡΠ΅Ρ ΠΎΠ΄Π½Ρ ΡΠΈΠ»ΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΡΡ Π²Π΅ΡΠ²Ρ Ρ ΡΠΈΠΏΠΎΠ²ΡΠΌΠΈ ΡΡΠ°ΠΌΠΌΠ°ΠΌΠΈ Paenibacillus nicotianae (98,3 %), Paenibacillus hordei (98,2 %), Paenibacillus kyungheensis (97,9 %), Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΠΊΠΎΡΠΎΡΠΎΠΉ Π·Π°Π½ΠΈΠΌΠ°Π΅Ρ ΠΎΠ±ΠΎΡΠΎΠ±Π»Π΅Π½Π½ΠΎΠ΅ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅. Π¨ΡΠ°ΠΌΠΌ Paenibacillus sp. ΠΠ‘-Π-17 ΠΌΠΎΠΆΠ΅Ρ Π½Π°ΠΉΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π² Π±ΠΈΠΎΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΊΠ°ΠΊ ΠΏΡΠΎΠ΄ΡΡΠ΅Π½Ρ Π²Π½Π΅ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄ΠΎΠ² ΠΈ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ², ΡΠ°ΡΡΠ΅ΠΏΠ»ΡΡΡΠΈΡ
ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΊΠ°ΠΊ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ ΠΌΠΈΠΊΡΠΎΠ±Π½ΠΎΠ³ΠΎ ΠΊΠΎΠ½ΡΠΎΡΡΠΈΡΠΌΠ° Π² ΡΠΎΡΡΠ°Π²Π΅ Π½ΠΎΠ²ΠΎΠΉ ΠΊΠΎΡΠΌΠΎΠ²ΠΎΠΉ Π΄ΠΎΠ±Π°Π²ΠΊΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ
ΠΠ½Π°Π»ΠΈΠ· ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ° Π³Π΅Π½Π° SlMYB12, Π΄Π΅ΡΠ΅ΡΠΌΠΈΠ½ΠΈΡΡΡΡΠ΅Π³ΠΎ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π· Ρ Π°Π»ΠΊΠΎΠ½-Π½Π°ΡΠΈΠ½Π³Π΅Π½ΠΈΠ½Π° Π² ΠΊΠΎΠΆΠΈΡΠ΅ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΡΠΎΠΌΠ°ΡΠ°, ΠΈ Π΅Π³ΠΎ Π²Π»ΠΈΡΠ½ΠΈΡ Π½Π° Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°
Efficiency in detecting of tomato forms with no chalcone-naringenin flavonoid in pink-fruited and yellow-fruited forms was evaluated using DNA markers for various polymorphisms of the SlMYB12 gene. The closest relationship between a phenotype with the transparent skin of fruits and a deletion in the promoter region of the SlMYB12 gene was shown. The highest efficiency in the detection of the recessive y allele of the regulatory SlMYB12 gene, leading to the chalcone-naringenin synthesis disruption and skin transparency, was established by a combination of markers MYB12-603delaF1/603del-aR6 (Myb-603del aF1/R6) and MYB12-603del-aF1/603del-aR5 (Myb12 aF1/R5). Fruit coloration peculiarities were shown depending on a combination of the structural alleles of a carotenoid biosynthesis pathway and SlMYB12 gene alleles. A combination of this y allele with the alleles of the gene of the lycopene-Ξ²-cyclase beta (b) and old gold crimson (ogc ) allows selecting pink and raspberry forms respectively. In tomato accessions with yellow and orange fruits, the y allele provides pale shades of the main coloration determined by carotenoid biosynthesis genes (yellow flesh (r), tangerine (t), Beta (B)). The presence of SNP T β C of the SlMYB12 gene (171476848 position of chromosome 1) was identified in 80 % of accessions with the transparent skin of fruits of the evaluated collection. The effect of the recessive y allele of the SlMYB12 gene on an increase in the lycopene concentration of tomato fruits in a combination with b, ogc alleles was shown. Using MAC methodsΒ by fruit quality genes, including the SlMYB12 gene, the cherry tomato variety Malinovyj koktel with a high lycopene accumulation was developed and included in the State RegisterΠΡΠ΅Π½Π΅Π½Π° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΡΠΎΡΠΌ ΡΠΎΠΌΠ°ΡΠ° Ρ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄Π° Ρ
Π°Π»ΠΊΠΎΠ½-Π½aΡΠΈΠ½Π³Π΅Π½ΠΈΠ½Π° Π² ΡΠΎΠ·ΠΎΠ²ΠΎΠΏΠ»ΠΎΠ΄Π½ΡΡ
ΠΈ ΠΆΠ΅Π»ΡΠΎΠΏΠ»ΠΎΠ΄Π½ΡΡ
ΡΠΎΡΠΌΠ°Ρ
Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΠΠ-ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΊ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ°ΠΌ Π³Π΅Π½Π° SlMYB12. ΠΠΎΠΊΠ°Π·Π°Π½Π° Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ΅ΡΠ½Π°Ρ ΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠ΅Π½ΠΎΡΠΈΠΏΠΎΠΌ Ρ ΠΏΡΠΎΠ·ΡΠ°ΡΠ½ΠΎΠΉ ΠΊΠΎΠΆΠΈΡΠ΅ΠΉ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΠΈ Π΄Π΅Π»Π΅ΡΠΈΠ΅ΠΉ Π² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π³Π΅Π½Π° SlMYB12. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΡΠ΅ΡΠ΅ΡΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π°Π»Π»Π΅Π»Ρ y ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π° SlMYB12, ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΠ΅Π³ΠΎ ΠΊ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΡΠΈΠ½ΡΠ΅Π·Π° Ρ
Π°Π»ΠΊΠΎΠ½-Π½aΡΠΈΠ½Π³Π΅Π½ΠΈΠ½Π° ΠΈ ΠΏΡΠΎΠ·ΡΠ°ΡΠ½ΠΎΡΡΠΈ ΠΊΠΎΠΆΠΈΡΡ, ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠ΅ΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² MYB12-603del-aF1/603del-aR6 (Myb-603del aF1/R6) ΠΈ MYB12-603del-aF1/603del-aR5 (Myb12 aF1/R5). ΠΠΎΠΊΠ°Π·Π°Π½Ρ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΠΎΠΊΡΠ°ΡΠΊΠΈ ΠΏΠ»ΠΎΠ΄ΠΎΠ² Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ ΡΡΡΡΠΊΡΡΡΠ½ΡΡ
Π°Π»Π»Π΅Π»Π΅ΠΉ ΠΏΡΡΠΈ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΈ Π°Π»Π»Π΅Π»Π΅ΠΉ Π³Π΅Π½Π° SlMYB12. Π‘ΠΎΡΠ΅ΡΠ°Π½ΠΈΠ΅ Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π°Π»Π»Π΅Π»Ρ Ρ Ρ Π°Π»Π»Π΅Π»ΡΠΌΠΈ Π³Π΅Π½Π° Π»ΠΈΠΊΠΎΠΏΠΈΠ½-Ξ²-ΡΠΈΠΊΠ»Π°Π·Ρ bΠ΅ta (b) ΠΈ old gold crimson (ogc ) ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΡΠΎΠ±ΡΠ°ΡΡ ΡΠΎΠ·ΠΎΠ²ΡΠ΅ ΠΈ ΠΌΠ°Π»ΠΈΠ½ΠΎΠ²ΡΠ΅ ΡΠΎΡΠΌΡ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. Π£ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΡΠΎΠΌΠ°ΡΠ° Ρ ΠΆΠ΅Π»ΡΠΎΠΉ ΠΈ ΠΎΡΠ°Π½ΠΆΠ΅Π²ΠΎΠΉ ΠΎΠΊΡΠ°ΡΠΊΠΎΠΉ ΠΏΠ»ΠΎΠ΄ΠΎΠ² Π°Π»Π»Π΅Π»Ρ Ρ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°Π΅Ρ Π±Π»Π΅Π΄Π½ΡΠ΅ ΠΎΡΡΠ΅Π½ΠΊΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
ΠΎΠΊΡΠ°ΡΠΎΠΊ, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΡΡ
Π³Π΅Π½Π°ΠΌΠΈ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² (yellow flesh (r), tangerine (t), Beta (B)). ΠΡΡΠ²Π»Π΅Π½ΠΎ Π½Π°Π»ΠΈΡΠΈΠ΅ SNP Π’ β Π‘ Π³Π΅Π½Π° SlMYB12 (ΠΏΠΎΠ·ΠΈΡΠΈΡ 71476848 Ρ
ΡΠΎΠΌΠΎΡΠΎΠΌΡ 1) Ρ 80 % ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Ρ ΠΏΡΠΎΠ·ΡΠ°ΡΠ½ΠΎΠΉ ΠΊΠΎΠΆΠΈΡΠ΅ΠΉ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΠΎΡΠ΅Π½ΠΈΠ²Π°Π΅ΠΌΠΎΠΉ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠ΅ΡΠ΅ΡΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π°Π»Π»Π΅Π»Ρ y Π³Π΅Π½Π° SlMYB12 Π½Π° ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΎΠΌΠ°ΡΠ° Π² ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΈ Ρ Π°Π»Π»Π΅Π»ΡΠΌΠΈ b, ogc . Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΠΠ‘ ΠΏΠΎ Π³Π΅Π½Π°ΠΌ ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΏΠ»ΠΎΠ΄ΠΎΠ², Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΠΏΠΎ Π³Π΅Π½Ρ SlMYB12, ΡΠΎΠ·Π΄Π°Π½ ΠΈ Π²ΠΊΠ»ΡΡΠ΅Π½ Π² ΠΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΡΠΉ ΡΠ΅Π΅ΡΡΡ ΡΠΎΡΡ ΡΠΎΠΌΠ°ΡΠ° ΡΠ΅ΡΡΠΈ ΠΠ°Π»ΠΈΠ½ΠΎΠ²ΡΠΉ ΠΊΠΎΠΊΡΠ΅ΠΉΠ»Ρ Ρ Π²ΡΡΠΎΠΊΠΈΠΌ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ΠΌ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°