40 research outputs found

    Role of foreign language teacher shaping students’ research skills

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    Β© 2015 Canadian Center of Science and Education. All rights reserved. Nowadays many foreign language teachers are not enough aware about the significance of the research component within their profile discipline, arguing that students even in their native language do not have enough use of fundamentals in their scientific professional activities. Therefore, this article is aimed to study the role of foreign language teacher when mastering students’ research skills in the process of learning English as a foreign language. The study results have confirmed that process of mastering students’ research skills when learning foreign language is directly connected with their teacher’s own research skill level. These article materials have practical value both for foreign language teachers and for students enrolled for foreign languages programs of education sciences faculties at high schools

    Pedagogical potential of the career guidance course β€œProfessional career planning” to form pupils and students’ self-determination in the integrated system β€œschoolβ€”vocational college”

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    Β© 2015, Canadian Center of Science and Education. All rights reserved. The article is aimed at revealing pedagogical potential of an elective career guidance course to form pupils and students’ professional self-determination in the integrated system β€œschoolβ€”vocational college”. The basic approach to research this problem is an integrative one that causes efficiency of pupils and students’ career guidance work in dual integrated system. The content of career guidance course β€œProfessional Career Planning” developed and presented in this article is aimed at pupils’ professional self-determination formation, consciousness and steady interest in choosing future profession. As for vocational college students, the course is directed towards the students’ choice of their own individual educational and career way, revealing their adaptation abilities to future professional activity. Materials of the article are of value for school and vocational college teachers while organizing and planning career guidance work at the educational institutions

    Features of formation of students foreign language competence in self-learning activity

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    Β© Canadian Center of Science and Education. This article is aimed to identify characteristics of the formation of students foreign language skills in the process of self-learning activity. It is proved that foreign language competences are formed as a complex of integrated steady students education as subjects of self-learning activity including: language, speech, compensatory, educational and informative, socio-cultural, communicative and projecting competences. The article submissions are of practical value to foreign language teachers in the organization and planning of students educational and self-learning activity aimed to establish and improve their foreign language competence

    Designing technology of English language teaching content based on international component

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    Β© 2015, Canadian Center of Science and Education. All rights reserved. This paper aims to develop designing technology of English language teaching content for students at higher education institution in the context of education internationalization. It highlighted the analytical, pre-designing, designing and correction-implementation stages as well as their successive phases to achieve a specific goal, i.e. English language teaching content designing in terms of international component. The materials of this paper are of practical value for teachers of foreign languages in the selecting and structuring the language teaching content in the context of education internationalization

    Π’Π›Π˜Π―ΠΠ˜Π• Π›Π˜ΠŸΠžΠ‘ΠžΠœΠΠ›Π¬ΠΠžΠ™ ЀОРМЫ Π‘Π’Π Π•ΠŸΠ’ΠžΠšΠ˜ΠΠΠ—Π« НА ΠžΠ‘Π ΠΠ—ΠžΠ’ΠΠΠ˜Π• Π”-Π”Π˜ΠœΠ•Π ΠžΠ’

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    Liposomal streptokinase (a mixture of free (66.3Β  %) and entrapped (23.7Β  %) drug) with a diameter of ~60 nm was prepared. The analysis of the D-dimers formation kinetics in the dog’s blood plasma showed that the liposomal streptokinase hada prolonged effect up to 180 minutes.ΠŸΠΎΠ»ΡƒΡ‡Π΅Π½Ρ‹ липосомы со стрСптокиназой с Ρ€Π°Π·ΠΌΠ΅Ρ€Π°ΠΌΠΈ ~60 Π½ΠΌ, ΠΏΡ€Π΅Π΄ΡΡ‚Π°Π²Π»ΡΡŽΡ‰ΠΈΠ΅ собой смСсь, ΡΠΎΡΡ‚ΠΎΡΡ‰ΡƒΡŽ ΠΈΠ· свободного (66,3Β  %) ΠΈ связанного (23,7Β  %) ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π°. На основании Π°Π½Π°Π»ΠΈΠ·Π° ΠΊΠΈΠ½Π΅Ρ‚ΠΈΠΊΠΈ образования Π”-Π΄ΠΈΠΌΠ΅Ρ€ΠΎΠ² Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡ€ΠΎΠ²ΠΈ собак установлСно, Ρ‡Ρ‚ΠΎ липосомальная Ρ„ΠΎΡ€ΠΌΠ° стрСптокиназы ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ‚ ΠΏΡ€ΠΎΠ»ΠΎΠ½Π³ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹ΠΌ эффСктом Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ 180 ΠΌΠΈΠ½

    ΠŸΠΎΠΊΠ°Π·Π°Ρ‚Π΅Π»ΠΈ продуктивности ΠΈ ΠΏΠΈΡ‚Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΠΉ цСнности Π·Π΅Ρ€Π½Π° Ρƒ Π³Π΅Π½ΠΎΡ‚ΠΈΠΏΠΎΠ² ΠΏΡˆΠ΅Π½ΠΈΡ†Ρ‹ с Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹ΠΌΠΈ аллСлями Π³Π΅Π½Π° NAM-Π’1

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    The identification of a functional NAM-B1 allele associated with a high content of grain protein and essential microelements in wheat relatives increased the distant hybridization significance for bread wheat nutritional value. The allelic polymorphism of the NAM-B1 gene in 22 wheat lines with a genetic material of T. dicoccoides, T. dicoccum, T. spelta, T. kiharΠ°e and their parental forms and the effects of NAM-B1 gene allelic variations on the content of grain protein and essential microelements and productivity traits (vegetation period 2017–2021) were evaluated. The functional NAM-B1 allele was identified only in the samples of wheat relatives among the parental forms. All parental varieties and most of introgressive lines (77.3 %) had a non-functional allele. The genotypes with the functional NAM-B1 allele were characterized by a higher plant height and tillering, but by lower spike productivity compared to the non-functional allele genotypes. The presence of the functional NAM-B1 allele provided a high level of grain protein and zinc content and never decreased significantly a thousand-kernel weight across all studied environments. The functional NAM-B1 allele introgression could be a resource for improving the grain wheat nutritional value.ΠžΠ±Π½Π°Ρ€ΡƒΠΆΠ΅Π½ΠΈΠ΅ Ρƒ сородичСй ΠΏΡˆΠ΅Π½ΠΈΡ†Ρ‹ Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ (Π΄ΠΈΠΊΠΎΠ³ΠΎ) аллСля Π³Π΅Π½Π° NAM-Π’1, ассоциированного с высоким содСрТаниСм Π±Π΅Π»ΠΊΠ° ΠΈ ΠΊΠ»ΡŽΡ‡Π΅Π²Ρ‹Ρ… микроэлСмСнтов Π² Π·Π΅Ρ€Π½Π΅, ΡƒΠ²Π΅Π»ΠΈΡ‡ΠΈΠ»ΠΎ Π·Π½Π°Ρ‡ΠΈΠΌΠΎΡΡ‚ΡŒ ΠΎΡ‚Π΄Π°Π»Π΅Π½Π½ΠΎΠΉ Π³ΠΈΠ±Ρ€ΠΈΠ΄ΠΈΠ·Π°Ρ†ΠΈΠΈ для ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΡ ΠΏΠΈΡ‚Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΠΉ цСнности Π·Π΅Ρ€Π½Π° T. aestivum L. Π˜Π·ΡƒΡ‡Π΅Π½ Π°Π»Π»Π΅Π»ΡŒΠ½Ρ‹ΠΉ состав Π³Π΅Π½Π° NAM-B1 Ρƒ 22 Π»ΠΈΠ½ΠΈΠΉ мягкой ΠΏΡˆΠ΅Π½ΠΈΡ†Ρ‹ с гСнСтичСским ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»ΠΎΠΌ T. dicoccoides, T. dicoccum, T. spelta, T. kiharΠ°e ΠΈ ΠΈΡ… Ρ€ΠΎΠ΄ΠΈΡ‚Π΅Π»ΡŒΡΠΊΠΈΡ… Ρ„ΠΎΡ€ΠΌ ΠΈ ΠΎΡ†Π΅Π½Π΅Π½ эффСкт Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹Ρ… Π°Π»Π»Π΅Π»Π΅ΠΉ Π³Π΅Π½Π° NAM-B1 Π½Π° содСрТаниС Π±Π΅Π»ΠΊΠ°, основных микроэлСмСнтов Π² Π·Π΅Ρ€Π½Π΅ ΠΈ ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΈ продуктивности (Π²Π΅Π³Π΅Ρ‚Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹Π΅ ΠΏΠ΅Ρ€ΠΈΠΎΠ΄Ρ‹ 2017–2021 Π³Π³.). Π‘Ρ€Π΅Π΄ΠΈ Ρ€ΠΎΠ΄ΠΈΡ‚Π΅Π»ΡŒΡΠΊΠΈΡ… Ρ„ΠΎΡ€ΠΌ Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½Ρ‹ΠΉ аллСль Π³Π΅Π½Π° NAM-B1 ΠΎΠ±Π½Π°Ρ€ΡƒΠΆΠ΅Π½ Ρ‚ΠΎΠ»ΡŒΠΊΠΎ Ρƒ ΠΎΠ±Ρ€Π°Π·Ρ†ΠΎΠ² Π²ΠΈΠ΄ΠΎΠ²-сородичСй. ВсС Ρ€ΠΎΠ΄ΠΈΡ‚Π΅Π»ΡŒΡΠΊΠΈΠ΅ сорта ΠΈ большая Ρ‡Π°ΡΡ‚ΡŒ интрогрСссивных Π»ΠΈΠ½ΠΈΠΉ (77,3 %) ΠΈΠΌΠ΅Π»ΠΈ ΠΌΡƒΡ‚Π°Π½Ρ‚Π½Ρ‹ΠΉ (Π½Π΅Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½Ρ‹ΠΉ) аллСль. Анализ срСдних ΠΌΠ½ΠΎΠ³ΠΎΠ»Π΅Ρ‚Π½ΠΈΡ… Π·Π½Π°Ρ‡Π΅Π½ΠΈΠΉ основных количСствСнных ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΎΠ² выявил, Ρ‡Ρ‚ΠΎ Π³Π΅Π½ΠΎΡ‚ΠΈΠΏΡ‹ с Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½Ρ‹ΠΌ Π°Π»Π»Π΅Π»Π΅ΠΌ NAM-Π’1 Ρ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ·ΠΎΠ²Π°Π»ΠΈΡΡŒ большСй высотой растСния ΠΈ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ΠΈΠ²Π½ΠΎΠΉ ΠΊΡƒΡΡ‚ΠΈΡΡ‚ΠΎΡΡ‚ΡŒΡŽ, Π½ΠΎ Π±ΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΈΠΌΠΈ показатСлями продуктивности колоса ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с Π³Π΅Π½ΠΎΡ‚ΠΈΠΏΠ°ΠΌΠΈ, нСсущими Π½Π΅Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½Ρ‹ΠΉ аллСль. УстановлСно, Ρ‡Ρ‚ΠΎ Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ аллСля Π΄ΠΈΠΊΠΎΠ³ΠΎ Ρ‚ΠΈΠΏΠ° обСспСчиваСт высокий ΡƒΡ€ΠΎΠ²Π΅Π½ΡŒ накоплСния Π±Π΅Π»ΠΊΠ° ΠΈ Ρ†ΠΈΠ½ΠΊΠ° Π² Π·Π΅Ρ€Π½Π΅ нСзависимо ΠΎΡ‚ ΠΏΠΎΠ³ΠΎΠ΄Π½Ρ‹Ρ… условий, ΠΈ ΠΏΡ€ΠΈ этом Π½Π΅ ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ сущСствСнному сниТСнию массы 1000 Π·Π΅Ρ€Π΅Π½. Показана ΡΡ„Ρ„Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ интрогрСссии Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ аллСля NAM-Π’1 ΠΎΡ‚ Π²ΠΈΠ΄ΠΎΠ²-сородичСй для ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΡ ΠΏΠΈΡ‚Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΠΉ цСнности Π·Π΅Ρ€Π½Π° мягкой ΠΏΡˆΠ΅Π½ΠΈΡ†Ρ‹

    Π€Π•Π ΠœΠ•ΠΠ’ΠΠ’Π˜Π’ΠΠΠ― ΠΠšΠ’Π˜Π’ΠΠžΠ‘Π’Π¬ Π‘ΠΠšΠ’Π•Π Π˜Π™ Π ΠžΠ”Π ARTHROBACTER И ИΠ₯ Π“Π•ΠΠžΠ’Π˜ΠŸΠ˜Π§Π•Π‘ΠšΠΠ― Π˜Π”Π•ΠΠ’Π˜Π€Π˜ΠšΠΠ¦Π˜Π―

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    Two-stage screening enabled one to define the composition of enzyme complexes produced by bacteria of genus ArthrobacterΒ in media with specific substrates and activity of individual constituents involved in the t ransformation of milk proteins,Β lipids and carbohydrates. It was found that enzyme complexes of Arthrobacter strains BIM B-2239, BIM B-2240, BIMΒ B-2241 and BIM B-2242 showed the most balanced activity of lipase, protease, Ξ²-galactosidase and glucose(xylose)isomeraseΒ components. Following the nucleotide sequence analysis of 16S rRNA gene the examined cultures were identified as ArthrobacterΒ sulfonivorans. 16S rRNA gene sequences over 1400 bp in size were deposited in GenBank database.Detailed investigation of conditions favoring the efficient hydrolysis of proteins and lipids by proteases and lipases producedΒ by Arthrobacter sulfonivorans strains BIM B-2239, BIM B-2240, BIM B-2241, BIM B-2242 and the transformation ofΒ milk lactose and derived hydrolysis products mediated by Ξ²-galactosidases and glucose(xylose)isomerases will allow one toΒ select microbial strain to develop the biotechnology of manufacturing hypoallergenic feed additive with prebiotic activityΒ from dairy substrates.Π’ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ двухступСнчатого скрининга ΠΎΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½Ρ‹ состав Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½Ρ‹Ρ… комплСксов, ΠΏΡ€ΠΎΠ΄ΡƒΡ†ΠΈΡ€ΡƒΠ΅ΠΌΡ‹Ρ… бактСриями Ρ€ΠΎΠ΄Π° Arthrobacter Π² срСдах с ΠΎ с пСцифичСским субстратом, ΠΈ Π° ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ ΠΈ Ρ… ΠΎ Ρ‚Π΄Π΅Π»ΡŒΠ½Ρ‹Ρ… ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚ΠΎΠ², ΡƒΡ‡Π°ΡΡ‚Π²ΡƒΡŽΡ‰ΠΈΡ…Β Π² трансформации Π±Π΅Π»ΠΊΠΎΠ², ΠΆΠΈΡ€ΠΎΠ² ΠΈ ΡƒΠ³Π»Π΅Π²ΠΎΠ΄ΠΎΠ² ΠΌΠΎΠ»ΠΎΠΊΠ°. УстановлСно, Ρ‡Ρ‚ΠΎ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ сбалансированными ΠΏΠΎ активности ΠΎΠ΄Π½ΠΎΠ²Ρ€Π΅ΠΌΠ΅Π½Π½ΠΎ Π»ΠΈΠΏΠ°Π·, ΠΏΡ€ΠΎΡ‚Π΅Π°Π·, Ξ²-Π³Π°Π»Π°ΠΊΡ‚ΠΎΠ·ΠΈΠ΄Π°Π· ΠΈ глюкозо(ксилозо)ΠΈΠ·ΠΎΠΌΠ΅Ρ€Π°Π· ΡΠ²Π»ΡΡŽΡ‚ΡΡ Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½Ρ‹Π΅ комплСксы ΡˆΡ‚Π°ΠΌΠΌΠΎΠ²Β Π‘Π˜Πœ Π’-2239, Π‘Π˜Πœ Π’-2240, Π‘Π˜Πœ Π’-2241 ΠΈ Π‘Π˜Πœ Π’-2242 Arthrobacter sp.ΠœΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π° Π½ΡƒΠΊΠ»Π΅ΠΎΡ‚ΠΈΠ΄Π½Ρ‹Ρ… ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚Π΅ΠΉ Π³Π΅Π½ΠΎΠ² 16S Ρ€Π ΠΠš ΡƒΠΊΠ°Π·Π°Π½Π½Ρ‹Π΅ Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Ρ‹ ΠΈΠ΄Π΅Π½Ρ‚ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Ρ‹ ΠΊΠ°ΠΊ Arthrobacter sulfonivorans. ΠŸΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΈΡ… Π³Π΅Π½ΠΎΠ² 16S Ρ€Π ΠΠš ΠΏΡ€ΠΎΡ‚ΡΠΆΠ΅Π½Π½ΠΎΡΡ‚ΡŒΡŽ Π±ΠΎΠ»Π΅Π΅ 1400 ΠΏ.Β ΠΎ. Π΄Π΅ΠΏΠΎΠ½ΠΈΡ€ΠΎΠ²Π°Π½Ρ‹ Π² Π±Π°Π·Π΅ Π΄Π°Π½Π½Ρ‹Ρ… GenBank.Π”Π΅Ρ‚Π°Π»ΡŒΠ½ΠΎΠ΅ исслСдованиС условий эффСктивного Π³ΠΈΠ΄Ρ€ΠΎΠ»ΠΈΠ·Π° Π±Π΅Π»ΠΊΠΎΠ² ΠΈ ΠΆΠΈΡ€ΠΎΠ² с участиСм ΠΏΡ€ΠΎΠ΄ΡƒΡ†ΠΈΡ€ΡƒΠ΅ΠΌΡ‹Ρ… ΡˆΡ‚Π°ΠΌΠΌΠ°ΠΌΠΈΒ Π‘Π˜Πœ Π’-2239, Π‘Π˜Πœ Π’-2240, Π‘Π˜Πœ Π’-2241 ΠΈ Π‘Π˜Πœ Π’-2242 Arthrobacter sp. Π»ΠΈΠΏΠ°Π· ΠΈ ΠΏΡ€ΠΎΡ‚Π΅Π°Π·, Π° Ρ‚Π°ΠΊΠΆΠ΅ трансформации Π»Π°ΠΊΡ‚ΠΎΠ·Ρ‹Β ΠΌΠΎΠ»ΠΎΠΊΠ° ΠΈ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ΠΎΠ² Π΅Π΅ Π³ΠΈΠ΄Ρ€ΠΎΠ»ΠΈΠ·Π° с участиСм Ξ²-Π³Π°Π»Π°ΠΊΡ‚ΠΎΠ·ΠΈΠ΄Π°Π· ΠΈ глюкозо(ксилозо)ΠΈΠ·ΠΎΠΌΠ΅Ρ€Π°Π· ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ‚ ΠΎΡ‚ΠΎΠ±Ρ€Π°Ρ‚ΡŒ ΡˆΡ‚Π°ΠΌΠΌ-ΠΏΡ€ΠΎΠ΄ΡƒΡ†Π΅Π½Ρ‚ для Ρ€Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ Π±ΠΈΠΎΡ‚Π΅Ρ…Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ получСния ΠΈΠ· ΠΌΠΎΠ»ΠΎΡ‡Π½ΠΎΠ³ΠΎ ΡΡ‹Ρ€ΡŒΡ Π³ΠΈΠΏΠΎΠ°Π»Π»Π΅Ρ€Π³Π΅Π½Π½ΠΎΠΉ биологичСски Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΠΉ ΠΊΠΎΡ€ΠΌΠΎΠ²ΠΎΠΉ Π΄ΠΎΠ±Π°Π²ΠΊΠΈ прСбиотичСского дСйствия

    Π’Ρ‹Π΄Π΅Π»Π΅Π½ΠΈΠ΅, характСристика ΠΈ молСкулярно-гСнСтичСская идСнтификация Π½ΠΎΠ²ΠΎΠ³ΠΎ ΡˆΡ‚Π°ΠΌΠΌΠ° Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΉ Paenibacillus species

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    Bacterial variant PS-K-17 was isolated from wheat grain contaminated by polysaccharide-producing microbiota for further characterization. It was found that the isolate grown on agar slants and in submerged culture on media with specific substrates synthesized beta-galactosidase, amylase, protease, pectinase, cellulase, beta-glucanase, lipase (esterase), alginase, extracellular polysaccharides, and pigments, probably carotenoids. Based on cultural-morphological and physiological-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene (access code MF443394 in GenBank) the bacterial culture was identified as Paenibacillus species PS-K-17. The studied isolate forms one phylogenetic branch with type strains Paenibacillus nicotianae (98.3 %), Paenibacillus hordei (98.2 %), Paenibacillus kyungheensis (97.9 %), holding wherein a separate position. Strain Paenibacillus sp. PS-K-17 may find use in biotechnology as a producer of extracellular polysaccharides and enzymes splitting plant polymeric substances as well as a component of microbial consortium-ingredient of a new complex feed additive.Из Π·Π΅Ρ€Π½Π° ΠΏΡˆΠ΅Π½ΠΈΡ†Ρ‹, ΠΊΠΎΠ½Ρ‚Π°ΠΌΠΈΠ½ΠΈΡ€ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΎΠ±Ρ€Π°Π·ΡƒΡŽΡ‰Π΅ΠΉ полисахарид ΠΌΠΈΠΊΡ€ΠΎΡ„Π»ΠΎΡ€ΠΎΠΉ, Π²Ρ‹Π΄Π΅Π»Π΅Π½Π° ΠΈ ΠΎΡ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ·ΠΎΠ²Π°Π½Π° ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π° Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΉ ПБ-К-17. УстановлСно, Ρ‡Ρ‚ΠΎ изолят Π½Π° Π°Π³Π°Ρ€ΠΈΠ·ΠΎΠ²Π°Π½Π½Ρ‹Ρ… срСдах ΠΈ Π² Π³Π»ΡƒΠ±ΠΈΠ½Π½ΠΎΠΉ ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π΅ со спСцифичСскими субстратами синтСзируСт Π±Π΅Ρ‚Π°-Π³Π°Π»Π°ΠΊΡ‚ΠΎΠ·ΠΈΠ΄Π°Π·Ρƒ, Π°ΠΌΠΈΠ»Π°Π·Ρƒ, ΠΏΡ€ΠΎΡ‚Π΅Π°Π·Ρƒ, ΠΏΠ΅ΠΊΡ‚ΠΈΠ½Π°Π·Ρƒ, Ρ†Π΅Π»Π»ΡŽΠ»Π°Π·Ρƒ, Π±Π΅Ρ‚Π°- Π³Π»ΡŽΠΊΠ°Π½Π°Π·Ρƒ, Π»ΠΈΠΏΠ°Π·Ρƒ (эстСразу), Π°Π»ΡŒΠ³ΠΈΠ½Π°Π·Ρƒ, Π° Ρ‚Π°ΠΊΠΆΠ΅ Π²Π½Π΅ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Π΅ полисахариды ΠΈ ΠΏΠΈΠ³ΠΌΠ΅Π½Ρ‚Ρ‹, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ, ΠΊΠ°Ρ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄Ρ‹. На основании ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π°Π»ΡŒΠ½ΠΎ-морфологичСских ΠΈ Ρ„ΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΎ-биохимичСских особСнностСй, Π° Ρ‚Π°ΠΊΠΆΠ΅ филогСнСтичСского Π°Π½Π°Π»ΠΈΠ·Π° Π½ΡƒΠΊΠ»Π΅ΠΎΡ‚ΠΈΠ΄Π½Ρ‹Ρ… ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚Π΅ΠΉ Π³Π΅Π½Π° 16S Ρ€Π ΠΠš (ΠΊΠΎΠ΄ доступа MF443394 Π² GenBank) Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ°Π»ΡŒΠ½Π°Ρ ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π° ΠΈΠ΄Π΅Π½Ρ‚ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π° ΠΊΠ°ΠΊ Paenibacillus species ПБ-К-17. Π˜ΡΡΠ»Π΅Π΄ΡƒΠ΅ΠΌΡ‹ΠΉ изолят ΠΎΠ±Ρ€Π°Π·ΡƒΠ΅Ρ‚ ΠΎΠ΄Π½Ρƒ Ρ„ΠΈΠ»ΠΎΠ³Π΅Π½Π΅Ρ‚ΠΈΡ‡Π΅ΡΠΊΡƒΡŽ Π²Π΅Ρ‚Π²ΡŒ с Ρ‚ΠΈΠΏΠΎΠ²Ρ‹ΠΌΠΈ ΡˆΡ‚Π°ΠΌΠΌΠ°ΠΌΠΈ Paenibacillus nicotianae (98,3 %), Paenibacillus hordei (98,2 %), Paenibacillus kyungheensis (97,9 %), Π² ΠΏΡ€Π΅Π΄Π΅Π»Π°Ρ… ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠΉ Π·Π°Π½ΠΈΠΌΠ°Π΅Ρ‚ обособлСнноС ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅. Π¨Ρ‚Π°ΠΌΠΌ Paenibacillus sp. ПБ-К-17 ΠΌΠΎΠΆΠ΅Ρ‚ Π½Π°ΠΉΡ‚ΠΈ ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π² Π±ΠΈΠΎΡ‚Π΅Ρ…Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΊΠ°ΠΊ ΠΏΡ€ΠΎΠ΄ΡƒΡ†Π΅Π½Ρ‚ Π²Π½Π΅ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… полисахаридов ΠΈ Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚ΠΎΠ², Ρ€Π°ΡΡ‰Π΅ΠΏΠ»ΡΡŽΡ‰ΠΈΡ… Ρ€Π°ΡΡ‚ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€Ρ‹, Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΊΠ°ΠΊ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚ ΠΌΠΈΠΊΡ€ΠΎΠ±Π½ΠΎΠ³ΠΎ консорциума Π² составС Π½ΠΎΠ²ΠΎΠΉ ΠΊΠΎΡ€ΠΌΠΎΠ²ΠΎΠΉ Π΄ΠΎΠ±Π°Π²ΠΊΠΈ комплСксного дСйствия

    Анализ ΠΏΠΎΠ»ΠΈΠΌΠΎΡ€Ρ„ΠΈΠ·ΠΌΠ° Π³Π΅Π½Π° SlMYB12, Π΄Π΅Ρ‚Π΅Ρ€ΠΌΠΈΠ½ΠΈΡ€ΡƒΡŽΡ‰Π΅Π³ΠΎ биосинтСз Ρ…Π°Π»ΠΊΠΎΠ½-Π½Π°Ρ€ΠΈΠ½Π³Π΅Π½ΠΈΠ½Π° Π² ΠΊΠΎΠΆΠΈΡ†Π΅ ΠΏΠ»ΠΎΠ΄ΠΎΠ² Ρ‚ΠΎΠΌΠ°Ρ‚Π°, ΠΈ Π΅Π³ΠΎ влияния Π½Π° Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°

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    Efficiency in detecting of tomato forms with no chalcone-naringenin flavonoid in pink-fruited and yellow-fruited forms was evaluated using DNA markers for various polymorphisms of the SlMYB12 gene. The closest relationship between a phenotype with the transparent skin of fruits and a deletion in the promoter region of the SlMYB12 gene was shown. The highest efficiency in the detection of the recessive y allele of the regulatory SlMYB12 gene, leading to the chalcone-naringenin synthesis disruption and skin transparency, was established by a combination of markers MYB12-603delaF1/603del-aR6 (Myb-603del aF1/R6) and MYB12-603del-aF1/603del-aR5 (Myb12 aF1/R5). Fruit coloration peculiarities were shown depending on a combination of the structural alleles of a carotenoid biosynthesis pathway and SlMYB12 gene alleles. A combination of this y allele with the alleles of the gene of the lycopene-Ξ²-cyclase beta (b) and old gold crimson (ogc ) allows selecting pink and raspberry forms respectively. In tomato accessions with yellow and orange fruits, the y allele provides pale shades of the main coloration determined by carotenoid biosynthesis genes (yellow flesh (r), tangerine (t), Beta (B)). The presence of SNP T β†’ C of the SlMYB12 gene (171476848 position of chromosome 1) was identified in 80 % of accessions with the transparent skin of fruits of the evaluated collection. The effect of the recessive y allele of the SlMYB12 gene on an increase in the lycopene concentration of tomato fruits in a combination with b, ogc alleles was shown. Using MAC methodsΒ by fruit quality genes, including the SlMYB12 gene, the cherry tomato variety Malinovyj koktel with a high lycopene accumulation was developed and included in the State RegisterΠžΡ†Π΅Π½Π΅Π½Π° ΡΡ„Ρ„Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ выявлСния Ρ„ΠΎΡ€ΠΌ Ρ‚ΠΎΠΌΠ°Ρ‚Π° с отсутствиСм Ρ„Π»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄Π° Ρ…Π°Π»ΠΊΠΎΠ½-Π½aΡ€ΠΈΠ½Π³Π΅Π½ΠΈΠ½Π° Π² Ρ€ΠΎΠ·ΠΎΠ²ΠΎΠΏΠ»ΠΎΠ΄Π½Ρ‹Ρ… ΠΈ ΠΆΠ΅Π»Ρ‚ΠΎΠΏΠ»ΠΎΠ΄Π½Ρ‹Ρ… Ρ„ΠΎΡ€ΠΌΠ°Ρ… с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ Π”ΠΠš-ΠΌΠ°Ρ€ΠΊΠ΅Ρ€ΠΎΠ² ΠΊ Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹ΠΌ ΠΏΠΎΠ»ΠΈΠΌΠΎΡ€Ρ„ΠΈΠ·ΠΌΠ°ΠΌ Π³Π΅Π½Π° SlMYB12. Показана Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ тСсная связь ΠΌΠ΅ΠΆΠ΄Ρƒ Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏΠΎΠΌ с ΠΏΡ€ΠΎΠ·Ρ€Π°Ρ‡Π½ΠΎΠΉ ΠΊΠΎΠΆΠΈΡ†Π΅ΠΉ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΠΈ Π΄Π΅Π»Π΅Ρ†ΠΈΠ΅ΠΉ Π² ΠΏΡ€ΠΎΠΌΠΎΡ‚ΠΎΡ€Π½ΠΎΠΉ области Π³Π΅Π½Π° SlMYB12. УстановлСна наибольшая ΡΡ„Ρ„Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ выявлСния рСцСссивного аллСля y рСгуляторного Π³Π΅Π½Π° SlMYB12, приводящСго ΠΊ Π½Π°Ρ€ΡƒΡˆΠ΅Π½ΠΈΡŽ синтСза Ρ…Π°Π»ΠΊΠΎΠ½-Π½aΡ€ΠΈΠ½Π³Π΅Π½ΠΈΠ½Π° ΠΈ прозрачности ΠΊΠΎΠΆΠΈΡ†Ρ‹, сочСтаниСм ΠΌΠ°Ρ€ΠΊΠ΅Ρ€ΠΎΠ² MYB12-603del-aF1/603del-aR6 (Myb-603del aF1/R6) ΠΈ MYB12-603del-aF1/603del-aR5 (Myb12 aF1/R5). ΠŸΠΎΠΊΠ°Π·Π°Π½Ρ‹ особСнности окраски ΠΏΠ»ΠΎΠ΄ΠΎΠ² Π² зависимости ΠΎΡ‚ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Ρ†ΠΈΠΈ структурных Π°Π»Π»Π΅Π»Π΅ΠΉ ΠΏΡƒΡ‚ΠΈ биосинтСза ΠΊΠ°Ρ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΈ Π°Π»Π»Π΅Π»Π΅ΠΉ Π³Π΅Π½Π° SlMYB12. Π‘ΠΎΡ‡Π΅Ρ‚Π°Π½ΠΈΠ΅ Π΄Π°Π½Π½ΠΎΠ³ΠΎ аллСля Ρƒ с аллСлями Π³Π΅Π½Π° Π»ΠΈΠΊΠΎΠΏΠΈΠ½-Ξ²-Ρ†ΠΈΠΊΠ»Π°Π·Ρ‹ bΠ΅ta (b) ΠΈ old gold crimson (ogc ) позволяСт ΠΎΡ‚ΠΎΠ±Ρ€Π°Ρ‚ΡŒ Ρ€ΠΎΠ·ΠΎΠ²Ρ‹Π΅ ΠΈ ΠΌΠ°Π»ΠΈΠ½ΠΎΠ²Ρ‹Π΅ Ρ„ΠΎΡ€ΠΌΡ‹ соотвСтствСнно. Π£ ΠΎΠ±Ρ€Π°Π·Ρ†ΠΎΠ² Ρ‚ΠΎΠΌΠ°Ρ‚Π° с ΠΆΠ΅Π»Ρ‚ΠΎΠΉ ΠΈ ΠΎΡ€Π°Π½ΠΆΠ΅Π²ΠΎΠΉ окраской ΠΏΠ»ΠΎΠ΄ΠΎΠ² аллСль Ρƒ обСспСчиваСт Π±Π»Π΅Π΄Π½Ρ‹Π΅ ΠΎΡ‚Ρ‚Π΅Π½ΠΊΠΈ основных окрасок, обусловлСнных Π³Π΅Π½Π°ΠΌΠΈ биосинтСза ΠΊΠ°Ρ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² (yellow flesh (r), tangerine (t), Beta (B)). ВыявлСно Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ SNP Π’ β†’ Π‘ Π³Π΅Π½Π° SlMYB12 (позиция 71476848 хромосомы 1) Ρƒ 80 % ΠΎΠ±Ρ€Π°Π·Ρ†ΠΎΠ² с ΠΏΡ€ΠΎΠ·Ρ€Π°Ρ‡Π½ΠΎΠΉ ΠΊΠΎΠΆΠΈΡ†Π΅ΠΉ ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΠΎΡ†Π΅Π½ΠΈΠ²Π°Π΅ΠΌΠΎΠΉ ΠΊΠΎΠ»Π»Π΅ΠΊΡ†ΠΈΠΈ. Показано влияниС рСцСссивного аллСля y Π³Π΅Π½Π° SlMYB12 Π½Π° ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΈ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ… Ρ‚ΠΎΠΌΠ°Ρ‚Π° Π² ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Ρ†ΠΈΠΈ с аллСлями b, ogc . Π‘ использованиСм ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² МАБ ΠΏΠΎ Π³Π΅Π½Π°ΠΌ качСства ΠΏΠ»ΠΎΠ΄ΠΎΠ², Π² Ρ‚ΠΎΠΌ числС ΠΏΠΎ Π³Π΅Π½Ρƒ SlMYB12, создан ΠΈ Π²ΠΊΠ»ΡŽΡ‡Π΅Π½ Π² ГосударствСнный рССстр сорт Ρ‚ΠΎΠΌΠ°Ρ‚Π° Ρ‡Π΅Ρ€Ρ€ΠΈ ΠœΠ°Π»ΠΈΠ½ΠΎΠ²Ρ‹ΠΉ ΠΊΠΎΠΊΡ‚Π΅ΠΉΠ»ΡŒ с высоким Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ΠΌ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°
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