12 research outputs found
Using Physiologically Based Pharmacokinetic Modeling to Assess the Risks of Failing Bioequivalence Criteria: a Tale of Two Ibuprofen Products
Measuring the Impact of Gastrointestinal Variables on the Systemic Outcome of Two Suspensions of Posaconazole by a PBPK Model
Interactions of polystyrene nanoplastics with in vitro models of the human intestinal barrier
3D Bioelectronic Model of the Human Intestine
Organ on chip (OoC) technologies have the potential to improve the translation
of promising therapies currently failing in clinical trials at great expense and
time due to dissimilarities between animal and human biology. Successful OoC
models integrate human cells within 3D tissues with surrounding biomolecular
components, and have benefited from the use of inert 3D gels and scaffolds
used as templates, prompting tissue formation. However, monitoring technologies used to assess tissue integrity and drug effects are ill adapted to 3D
biology. Here, a tubular electroactive scaffold serves as a template for a 3D
human intestine, and enables dynamic electrical monitoring of tissue formation
over 1 month. Cell- and extracellular matrix component-invoked changes in the
properties of the scaffold alleviate the need for posthoc placement of invasive
metallic electrodes or downstream analyses. Formation of in vivo-like stratified
and polarized intestinal tissue compete with lumen contrasts with other quasi3D models of the intestine using rigid porous membrane to separate cell types.
These results provide unprecedented real-time information on tissue formation with highly sensitive multimodal operation, thanks to dual electrode and
transistor operation. This device and the methodology for tissue growth within
it represents a paradigm shift for disease modeling and drug discover