Magnetic Moment of the Fragmentation Aligned 61Fe(9/2)+ Isomer

We report on the g factor measurement of the isomer in $^{61}Fe$ ($E^{*}=861 keV$). The isomer was produced and spin-aligned via a projectile-fragmentation reaction at intermediate energy, the Time Dependent Perturbed Angular Distribution (TDPAD) method being used for the measurement of the g factor. For the first time, due to significant improvements of the experimental technique, an appreciable residual alignment of the isomer has been observed, allowing a precise determination of its g factor: $g=-0.229(2)$. Comparison of the experimental g factor with shell-model and mean field calculations confirms the $9/2^+$ spin and parity assignments and suggests the onset of deformation due to the intrusion of Nilsson orbitals emerging from the $\nu g_{9/2}$.Comment: 4 figures. Submitted to Phys. Rev. Let

MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering.

Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non-homologous end-joining (NHEJ) or homologous recombination (HR) DNA repair pathways. However, a direct involvement of these pathways has remained unclear. Here, we show that the silencing of many HR factors enhanced plasmid concatemer formation and stable expression of the gene of interest in Chinese hamster ovary (CHO) cells, while the inhibition of NHEJ had no effect. However, genomic integration was decreased by the silencing of specific HR components, such as Rad51, and DNA synthesis-dependent microhomology-mediated end-joining (SD-MMEJ) activities. Genome-wide analysis of the integration loci and junction sequences validated the prevalent use of the SD-MMEJ pathway for transgene integration close to cellular genes, an effect shared with matrix attachment region (MAR) DNA elements that stimulate plasmid integration and expression. Overall, we conclude that SD-MMEJ is the main mechanism driving the illegitimate genomic integration of foreign DNA in CHO cells, and we provide a recombination engineering approach that increases transgene integration and recombinant protein expression in these cells. Biotechnol. Bioeng. 2017;114: 384-396. © 2016 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc

Precise DEM extraction from Svalbard using 1936 high oblique imagery

Stretching time series further in the past with the best possible accuracy is essential to the understanding of climate change impacts and geomorphological processes evolving on decadal-scale time spans. In the first half of the twentieth century, large parts of the polar regions were still unmapped or only superficially so. To create cartographic data, a number of historic photogrammetric campaigns were conducted using oblique imagery, which is easier to work with in unmapped environments as collocating images is an easier task for the human eye given a more familiar viewing angle and a larger field of view. Even if the data obtained from such campaigns gave a good baseline for the mapping of the area, the precision and accuracy are to be considered with caution. Exploiting the possibilities arising from modern image processing tools and reprocessing the archives to obtain better data is therefore a task worth the effort. The oblique angle of view of the data is offering a challenge to classical photogrammetric tools, but the use of modern structure-from-motion (SfM) photogrammetry offers an efficient and quantitative way to process these data into terrain models. In this paper, we propose a good practice method for processing historical oblique imagery using free and open source software (MicMac and Python) and illustrate the process using images of the Svalbard archipelago acquired in 1936 by the Norwegian Polar Institute. On these data, our workflow provides 5&thinsp;m resolution, high-quality elevation data (SD&thinsp;2&thinsp;m for moderate terrain) as well as orthoimages that allow for the reliable quantification of terrain change when compared to more modern data.</p

Mise en place de la Maîtrise Statistique des Procédés pour les contrôles pré-traitement par imagerie portale pour la technique VMAT

National audienc

The HPS electromagnetic calorimeter

The Heavy Photon Search experiment (HPS) is searching for a new gauge boson, the so-called “heavy photon.” Through its kinetic mixing with the Standard Model photon, this particle could decay into an electron-positron pair. It would then be detectable as a narrow peak in the invariant mass spectrum of such pairs, or, depending on its lifetime, by a decay downstream of the production target. The HPS experiment is installed in Hall-B of Jefferson Lab. This article presents the design and performance of one of the two detectors of the experiment, the electromagnetic calorimeter, during the runs performed in 2015–2016. The calorimeter's main purpose is to provide a fast trigger and reduce the copious background from electromagnetic processes through matching with a tracking detector. The detector is a homogeneous calorimeter, made of 442 lead-tungstate (PbWO4) scintillating crystals, each read out by an avalanche photodiode coupled to a custom trans-impedance amplifier

Quadrupole deformations of neutron-drip-line nuclei studied within the Skyrme Hartree-Fock-Bogolyubov approach

We introduce a local-scaling point transformation to allow for modifying the asymptotic properties of the deformed three-dimensional Cartesian harmonic oscillator wave functions. The resulting single-particle bases are very well suited for solving the Hartree-Fock-Bogoliubov equations for deformed drip-line nuclei. We then present results of self-consistent calculations performed for the Mg isotopes and for light nuclei located near the two-neutron drip line. The results suggest that for all even-even elements with $Z$=10--18 the most weakly-bound nucleus has an oblate ground-state shape.Comment: 20 pages, 7 figure

Land cover, land use and malaria in the Amazon: a systematic literature review of studies using remotely sensed data

The nine countries sharing the Amazon forest accounted for 89% of all malaria cases reported in the Americas in 2008. Remote sensing can help identify the environmental determinants of malaria transmission and their temporo-spatial evolution. Seventeen studies characterizing land cover or land use features, and relating them to malaria in the Amazon subregion, were identified. These were reviewed in order to improve the understanding of the land cover/use class roles in malaria transmission. The indicators affecting the transmission risk were summarized in terms of temporal components, landscape fragmentation and anthropic pressure. This review helps to define a framework for future studies aiming to characterize and monitor malaria