Characterization of the transverse relaxation rates in lipid bilayers

The 2H NMR transverse relaxation rates of a deuterated phospholipid bilayer reflect slow motions in the bilayer membrane. A study of dimyristoyl lecithin specifically deuterated at several positions of the hydrocarbon chains indicates that these motions are cooperative and are confined to the hydrocarbon chains of the lipid bilayer. However, lipid head group interactions do play an important role in modulating the properties of the cooperative fluctuations of the hydrocarbon chains (director fluctuations), as evidenced by the effects of various lipid additives on the 2H NMR transverse relaxation rates of the dimyristoyl lecithin bilayer

Histidine is the axial ligand to cytochrome alpha 3 in cytochrome c oxidase

The nitric oxide-bound complexes of reduced yeast cytochrome c oxidase incorporated with [1,3-15N2]histidine have been investigated by EPR spectroscopy. The results of this study have allowed the unambiguous identification of histidine as the endogenous axial ligand to cytochrome alpha 3

NMR studies of membrane structure and dynamics

Over the past decade, there has been considerable interest in the motional state of the phospholipid bilayer membrane. The motivation underlying these efforts has been the contention that the phospholipid bilayer is the basic matrix in which membrane proteins are embedded to form the biological membrane, and that the permeability and mechanical properties of the membrane, as well as the enzymatic activity of membrane proteins, are dependent upon the fluidity of the bilayer, especially the motional state of the hydrocarbon chains

Structures and proton-pumping strategies of mitochondrial respiratory enzymes

Enzymes of the mitochondrial respiratory chain serve as proton pumps, using the energy made available from electron transfer reactions to transport protons across the inner mitochondrial membrane and create an electrochemical gradient used for the production of ATP. The ATP synthase enzyme is reversible and can also serve as a proton pump by coupling ATP hydrolysis to proton translocation. Each of the respiratory enzymes uses a different strategy for performing proton pumping. In this work, the strategies are described and the structural bases for the action of these proteins are discussed in light of recent crystal structures of several respiratory enzymes. The mechanisms and efficiency of proton translocation are also analyzed in terms of the thermodynamics of the substrate transformations catalyzed by these enzymes

Proton translocation in proteins

The active transport of protons across the low dielectric barrier imposed by biological membranes is accomplished by a plethora of proteins that span the ca. 40 Å of the phospholipid bilayer. The free energy derived from the proton electrochemical potential established by the translocation of these protons can subsequently be used to drive vital chemical reactions of the cell, such as ATP synthesis and cell locomotion. Membrane-bound proton translocating proteins have now been found for a variety of organisms and tissues (1). The driving force for proton pumping in these proteins is supplied by numerous mechanisms, including light absorption (e.g. bacteriorhodopsin) (2a,b), ligand binding (e.g. ATPase) (3), and electrochemistry (e.g. electron transfer through cytochrome c oxidase) (4). Thus nature has devised a variety of methods for supplying the energy required for proton pumping by these proteins. Such diversity notwithstanding, the proteins most likely share some common elements of structure and mechanism that allow them to function as proton pumps. A number of theoretical mechanisms have been put forth for both general proton translocation (5-7) and for energy coupling in specific proton pumps. However, despite almost three decades of intensive research, the details of the mechanism(s) and structural requirements for proton pumping remain largely unresolved. To some extent this is the result of the paucity of structural information available for integral membrane proteins. This situation may soon improve as a result of advances in protein methodologies that have allowed several integral membrane proteins to be successfully crystalized (8), and the increased use of genetic engineering to obtain recombinant proton translocating proteins that will offer an opportunity to assess the importance of specific amino acids for the proton translocation process (9)

LEXTALE_CH: A quick, character-based proficiency test for Mandarin Chinese

Research in second language acquisition suggests that objective performance-based assessments may provide more reliable and valid measures of second language proficiency than subjective self-ratings. To measure proficiency in English as a second language, a quick, vocabulary-based test called LexTALE (Lexical Test for Advanced Learners of English) was developed and shown to be able to differentiate between various levels of English proficiency. Following in the line of adaptations of this test for other languages, we created a character-based adaptation for Mandarin Chinese: LEXTALE_CH. In this paper, we discuss the development and validation of LEXTALE_CH in detail. In short, LEXTALE_CH can discriminate between high and low levels of Mandarin proficiency and is sensitive to the significant differences in vocabulary size between native speakers and second language learners of Mandarin; further, it takes only a few minutes to administer and is simple to score, making it a practical tool for low-stakes estimation of Mandarin proficiency.http://www.lingref.com/bucld/42/BUCLD42-09.pdfPublished versio

Unusual association of ST-T abnormalities, myocarditis and cardiomyopathy with H1N1 influenza in pregnancy: two case reports and review of the literature.

Introduction Myocarditis is rarely reported as an extra-pulmonary manifestation of influenza while pregnancy is a rare cause of cardiomyopathy. Pregnancy was identified as a major risk factor for increased mortality and morbidity due to H1N1 influenza in the pandemic of 2009 to 2010. However, to the best of our knowledge there are no previous reports in the literature linking H1N1 with myocarditis in pregnancy. Case presentation We report the cases of two pregnant Caucasian women (aged 29 and 30), with no pre-existing illness, presenting with respiratory manifestations of H1N1 influenza virus infection in their third trimester. Both women developed evidence of myocarditis. One woman developed acute respiratory distress syndrome, almost reaching the point of requiring extra-corporeal membrane oxygenation, and subsequently developed persistent cardiomyopathy; the other recovered without any long-term consequence. Conclusions While it is not possible to ascertain retrospectively if myocarditis was caused by either infection with H1N1 virus or as a result of pregnancy (in the absence of endomyocardial biopsies), the significant association with myocardial involvement in both women demonstrates the increased risk of exposure to H1N1 influenza virus in pregnant women. This highlights the need for health care providers to increase awareness amongst caregivers to target this 'at risk' group aggressively with vaccination and prompt treatment

A physical-space version of the stretched-vortex subgrid-stress model for large-eddy simulation

A physical-space version of the stretched-vortex subgrid-stress model is presented and applied to large-eddy simulations of incompressible flows. This version estimates the subgrid-kinetic energy required for evaluation of the subgrid-stress tensor using local second-order structure-function information of the resolved velocity field at separations of order the local cell size. A relation between the structure function and the energy spectrum is derived using the kinematic assumptions of the stretched-vortex model for locally homogeneous anisotropic turbulence. Results of large-eddy simulations using this model are compared to experimental and direct numerical simulation data. Comparisons are shown for the decay of kinetic energy and energy spectra of decaying isotropic turbulence and for mean velocities, root-mean-square velocity fluctuations and turbulence-kinetic energy balances of channel flow at three different Reynolds numbers

Crossover from the pair contact process with diffusion to directed percolation

Crossover behaviors from the pair contact process with diffusion (PCPD) and the driven PCPD (DPCPD) to the directed percolation (DP) are studied in one dimension by introducing a single particle annihilation/branching dynamics. The crossover exponents $\phi$ are estimated numerically as $1/\phi \simeq 0.58\pm0.03$ for the PCPD and $1/\phi \simeq 0.49 \pm 0.02$ for the DPCPD. Nontriviality of the PCPD crossover exponent strongly supports non-DP nature of the PCPD critical scaling, which is further evidenced by the anomalous critical amplitude scaling near the PCPD point. In addition, we find that the DPCPD crossover is consistent with the mean field prediction of the tricritical DP class as expected