In vivo biochemical evaluations of some ß-lactam group antibiotics on glutathione reductase and glutathione S- transferase enzyme activities

PubMed: 31207309Objectives: The aim of this study was to investigate whether some of the cephalosporin group antibiotics have inhibition effects on GR and GST enzymes with important functions in the metabolic pathway. Methods: In this study, some selected cephalosporin group antibiotics on GST and GR enzyme was carried out using 96 rats. 16 groups (16 × 6) were created from these rats, divided to another 4 groups (4 × 24). The resulting groups were named as sham groups, cefazolin groups, cefuroxime groups and cefoperazone groups, respectively. The antibiotics used were injected to cefazolin, cefuroxime and cefoperazone groups. The inhibition effects of the antibiotics were measured in the different time intervals (1st, 3th, 5th, 7th). The statistical investigation of the results was performed using the SPSS software program. Results: Results revealed the complex effects of the tested substances on GR and GST activity at different time intervals and in different tissues (p < 0.05). This indicated that the tested substances could be exposed to different interactions in vivo. Conclusion: The tested antibiotics showed some significant inhibition effects on the GST and GR enzyme activity in some tissues of brain, eye and muscle. The interaction of enzyme - the drug is a key factor to highlight the toxicological mechanism. For this reason, the results obtained from in vivo experiments are crucial to explane the physiological properties of the enzymes. © 2019This study was supported by Scientific Research Fund of Iğdır University. Project Number: 2017-SBE-A11. A certain part of this study was carried out in Igdır University Research Laboratory Practice and Research Center

Investigation of the effects of cephalosporin antibiotics on glutathione S-transferase activity in different tissues of rats in vivo conditions in order to drug development research

Glutathione S-transferases are multifunctional enzymes for the cellular defense against xenobiotics and provide protection for organism. In this study, the inhibition effects of some antibiotics were investigated against GST obtained from albino-rats kidney, liver, and heart tissues. Ninety-six albino-rats were randomly divided into 16 groups (n:6). The first four groups were control groups that were administrated blank enjection and decapitated at 1–7 h. The other groups were administrated the antibiotics. In all tissues, GST activity was increased in antibiotics groups at 1st and 3rd hours compared to control groups, while it began to fall at 5th and 7th hours (p <.05). In kidney tissues, it was lower than the same control group the cefuroxime and cefoperazone groups at 7th hours (p <.05). In addition, almost all antibiotic groups of kidney tissues had higher GST activity at all hours than those of control groups, but it was higher only at 5th hours in heart tissues (p <.05). © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group

Influence of some ß-lactam drugs on selected antioxidant enzyme and lipid peroxidation levels in different rat tissues

PubMed: 31060396Antioxidant enzymes play an important role in body defense and free radical removal. Cephalosporins are ß-lactam antibiotics. In this work, the effects of cefazolin, cefuroxime and cefoperazone which are cephalosporins on some selected antioxidant enzyme and levels of malondialdehyde (MDA) as lipid peroxidation product were investigated in kidney, liver, and brain tissues of albino female rats. Ninety-six albino rats were randomly divided into 16 groups of equal number (n = 6). 50 mg/kg cefazolin, 25 mg/kg cefuroxime, and 100 mg/kg cefoperazone were injected intraperitoneally to the groups (5th–8th and 9th–12th, and 13th–16th groups), respectively. The changes in glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), peroxidase (POD), and glutathione peroxidase (GSH-Px) levels were studied in each time point group and a time-dependent manner (at the 1st, 3rd, 5th and 7th hour). In addition, MDA levels were examined in all the tissues. The drugs evaluated in this study had different effects on the same enzyme in different tissues depending on time. MDA levels especially in cefazolin and cefoperazone experiments were lower in all the tissues; however, MDA levels were higher in brain and kidney tissues in the cefuroxime groups in a time-dependent manner (p < 0.05). These results revealed the complex effects of the tested drugs on different tissues at different time points. Therefore, the dose and use of these drugs should be adjusted correctly. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.2018-SBE-A11This study was supported by Scientific Research Fund of I?d?r University. Project Number: 2018-SBE-A11

Zaprinast and avanafil increase the vascular endothelial growth factor, vitamin D(3), bone morphogenic proteins 4 and 7 levels in the kidney tissue of male rats applied the glucocorticoid

PMID = 3242139

Stimulating effects of vardenafil, tadalafil, and udenafil on vascular endothelial growth factor, angiogenesis, vitamin D(3), bone morphogenic proteins in ovariectomized rats

PMID = 3231492

The effects of some cephalosporins on acetylcholinesterase and glutathione S-transferase: an in vivo and in vitro study

PubMed: 29564935Background: Glutathione S-transferase (GST) and acetylcholinesterase (AChE) are important enzymes in the metabolism. GSTs are primarily available in phase II metabolism. AChE is vital for neurodegenerative disorders. Subjects and methods: The in vitro and in vivo effects of cefoperazone sodium (CFP), cefuroxime (CXM), and cefazolin (CZO) were investigated on GST and AChE activity in the present study. GST was purified using Glutathione-Agarose affinity chromatography. Results:K i constants of CFP, CXM, and CZO were 0.1392 ± 0.02, 1.5179 ± 0.33, and 1.006 ± 0.11 mM for GST and 0.3010 ± 0.07, 0.3561 ± 0.09, and 0.3844 ± 0.04 mM, for AChE, respectively. The most effective inhibitor was CFP for both enzymes in in vitro. CZO (50 mg/kg), CXM (25 mg/kg), and CFP (100 mg/kg) inhibit in vivo GST and AChE activities. CXM had the most effective in vivo inhibition on AChE and GST. Conclusions: CZO, CXM, and CFP are effective AChE and GST inhibitors in both in vitro and in vivo. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.2016-SBE-B08This work was financially supported by Ig?dır University Scientific Research Projects Coordination Commission with project number [2016-SBE-B08]

The susceptibility to autoxidation of erythrocytes in diabetic mice: Effects of melatonin and pentoxifylline

PMID = 2907724

Within-subject and between-subject biological variation of first morning void urine amino acids in 12 healthy subjects

PMID = 3232415

The levels of nitrite, nitrate and lipid peroxidation in diabetic mouse brain: the effect of melatonin and pentoxifylline

PMID = 3207014

Investigation of the effects of some sulfonamides on acetylcholinesterase and carbonic anhydrase enzymes

WOS: 000467327900002PubMed ID: 30811749Human carbonic anhydrase I and II isoenzymes (hCA I and II) and acetylcholinesterase (AChE) are important metabolic enzymes that are closely associated with various physiological and pathological processes. In this study, we investigated the inhibition effects of some sulfonamides on hCA I, hCA II, and AChE enzymes. Both hCA isoenzymes were purified by Sepharose-4B-L-Tyrosine-5-amino-2-methylbenzenesulfonamide affinity column chromatography with 1393.44 and 1223.09-folds, respectively. Also, some inhibition parameters including IC50 and K-i values were determined. Sulfonamide compounds showed IC50 values of in the range of 55.14 to 562.62 nM against hCA I, 55.99 to 261.96 nM against hCA II, and 98.65 to 283.31 nM against AChE. K-i values were in the range of 23.40 +/- 9.10 to 365.35 +/- 24.42 nM against hCA I, 45.87 +/- 5.04 to 230.08 +/- 92.23 nM against hCA II, and 16.00 +/- 45.53 to 157.00 +/- 4.02 nM against AChE. As a result, sulfonamides had potent inhibition effects on these enzymes. Therefore, we believe that these results may contribute to the development of new drugs particularly in the treatment of some disorders