In-Vivo Anti-Inflammatory Activity of Kelubut Leaf Ethyl Acetate Extract (Passiflora foetida L.) from Samarinda City

Anti-inflammatory plants, namely Kelubut (Passiflora foetida L.), are widely found in various regions in Indonesia, including in Central Kalimantan. The composition of the main chemical compounds includes alkaloids, phenols, glycosides, flavonoids, and cyanogenic compounds. Flavonoids show over a hundred bioactivity, including antipyretic, analgesic, and anti-inflammatory. This study aims to determine the anti-inflammatory activity of Kelubut leaf extract (Passiflora foetda L.) against mice (Mus musculus).This research was carried out by The Pretest and Posttest Control Group Design with 5 treatment groups, namely negative control, positive control, and ethyl acetate extract of Kelubut leaves with 3 doses, namely 250 mg/KgBW, 125mg/KgBW and 62,5 mg/KgBB. Prior to the treatment, each treatment group was induced by carrageenin by sub-planar injection into the sole of the left leg of the mouse. The edema developed rapidly and persisted for 6 hours. After being induced by carrageenin, it was left for 30 minutes, and the edema volume was measured every 30 to 120 minutes.Based on the results of the percentage inhibition of all groups of ethyl acetate extract of Kelubut leaves, the leaves had anti-inflammatory activity, with different resulting abilities. The percentage ofinflammation inhibition by ethyl acetate extract at a dose of 250 mg/KgBW was 92.78%, at a dose of  125 mg/KgBW was 91.76%, and at a dose of 62.5 mg/KgBW was 84.61%. Based on the results, it can be seen that the ethyl acetate extract group of Kelubut leaves at a dose of 250 mg/KgBW had the greatest inflammatory inhibition activity compared to a dose of 125 mg/KgBW and 62.5 mg/KgBW