38 research outputs found
Recommended from our members
Troglitazone reduces the expression of PPARγ while stimulating that of PPARα in mononuclear cells in obese subjects
Recommended from our members
Glucose Challenge Stimulates Reactive Oxygen Species (ROS) Generation by Leucocytes
Diabetes mellitus is associated with increased ROS generation,
oxidative injury and obesity. To elucidate the relationship between
nutrition and ROS generation, we have investigated the effect of
glucose challenge on ROS generation by leucocytes, p47phox
protein, a key protein in the enzyme NADPH oxidase and α-tocopherol
levels. Blood samples were drawn from 14 normal subjects prior to, at
1, 2 and 3 h following ingestion of 75 g glucose. ROS generation by
polymorphonuclear leucocytes (PMNL) and mononuclear cells (MNC)
increased to a peak of 244 ± 42% and 233 ± 34% of the basal
respectively at 2 h. The levels of p47phox in MNC
homogenates increased significantly at 2 h and 3 h after glucose
intake. α-Tocopherol levels decreased significantly at 1 h, 2 h, and
3 h. We conclude that glucose intake stimulates ROS generation and
p47phox of NADPH oxidase; increases oxidative load and
causes a fall in α-tocopherol concentration
Both lipid and protein intakes stimulate increased generation of reactive oxygen species by polymorphonuclear leukocytes and mononuclear cells
Recommended from our members