1 research outputs found
Three Novel Pigmentation Mutants Generated by Genome-Wide Random ENU Mutagenesis in the Mouse
Three mutant mice with pigmentation phenotypes were recovered from a genomewide
random mouse chemical mutagenesis study. White toes (Whto; MGI:1861986),
Belly spot and white toes (Bswt; MGI:2152776) and Dark footpads 2 (Dfp2;
MGI:1861991) were identified following visual inspection of progeny from a male
exposed to the point mutagen ethylnitrosourea (ENU). In order to rapidly localize
the causative mutations, genome-wide linkage scans were performed on pooled
DNA samples from backcross animals for each mutant line. Whto was mapped to
proximal mouse chromosome (Mmu) 7 between Cen (the centromere) and D7Mit112
(8.0 cM from the centromere), Bswt was mapped to centric Mmul between D1Mit214
(32.1 cM) and D1Mit480 (32.8 cM) and Dfp2 was mapped to proximalMmu4 between
Cen and D4Mit18 (5.2 cM). Whto, Bswt and Dfp2 may provide novel starting
points in furthering the elucidation of genetic and biochemical pathways relevant
to pigmentation and associated biological processes