Animal Models in Myometrial Activity Research: Morphofunctional Features, Role of Oxytocin

One of the main functions of the reproductive system is providing the physiological process, which occurs by caudal spread of excitability of smooth muscle tissue and ensures delivery of the fetus. The results of this work confirm the importance of blood supply in automatism of the ovarian horn areas, which are the leading regions in propagation of electrical waves and coordination of other rhythmogenic loci. Morphofunctional methods have shown that the ovarian horn areas have strong vascularization, which is confirming the pronounced electrical activity of these loci. Oxytocin has a central role in changing of excitation patterns. Increasing hormone concentrations (10–2 μg/kg, 10–1 μg/kg, 1 μg/kg, 10 μg/kg) resulted in increase of the bursting activity duration of all studied myometrial areas. At the same time, rise in the frequency of spike rhythmogenesis was observed only at a dose of 1 μg/kg. Morpho-histochemical analysis revealed the existence of atypical cells with a high level of Ca2+-dependent acid phosphatase in both distal rhythmogenic ends of the horn. However, the ovarian horn area had the greatest enzymatic activity. Thus, the obtained data give good reason to conclude that the ovarian horn area has a leading role in the myometrium

Alternating hemiplegia of childhood: evolution over time and mouse model corroboration

Alternating hemiplegia of childhood is a rare neurodevelopmental disorder caused by ATP1A3 mutations. Some evidence for disease progression exists, but there are few systematic analyses. Here, we evaluate alternating hemiplegia of childhood progression in humans and in the D801N knock-in alternating hemiplegia of childhood mouse, Mashlool, model. This study performed an ambidirectional (prospective and retrospective data) analysis of an alternating hemiplegia of childhood patient cohort (n = 42, age 10.24 ± 1.48 years) seen at one US centre. To investigate potential disease progression, we used linear mixed effects models incorporating early and subsequent visits, and Wilcoxon Signed Rank test comparing first and last visits. Potential early-life clinical predictors were determined via multivariable regression. We also compared EEG background at first encounter and at last follow-up. We then performed a retrospective confirmation study on a multicentre cohort of alternating hemiplegia of childhood patients from France (n = 52). To investigate disease progression in the Mashlool mouse, we performed behavioural testing on a cohort of Mashlool- mice at prepubescent and adult ages (n = 11). Results: US patients, over time, demonstrated mild worsening of non-paroxysmal disability index scores, but not of paroxysmal disability index scores. Increasing age was a predictor of worse scores: P < 0.0001 for the non-paroxysmal disability index, intellectual disability scale and gross motor scores. Earliest non-paroxysmal disability index score was a predictor of last visit non-paroxysmal disability index score (P = 0.022), and earliest intellectual disability score was a predictor of last intellectual disability score (P = 0.035). More patients with EEG background slowing were noted at last follow-up as compared to initial (P = 0.015). Similar worsening of disease with age was also noted in the French cohort: age was a significant predictor of non-paroxysmal disability index score (P = 0.001) and first and last non-paroxysmal disability index score scores significantly differed (P = 0.002). In animal studies, adult Mashlool mice had, as compared to younger Mashlool mice, (i) worse balance beam performance; (ii) wider base of support; (iii) higher severity of seizures and resultant mortality; and (iv) no increased predisposition to hemiplegic or dystonic spells. In conclusion, (i) non-paroxysmal alternating hemiplegia of childhood manifestations show, on average over time, progression associated with severity of early-life non-paroxysmal disability and age. (ii) Progression also occurs in Mashlool mice, confirming that ATP1A3 disease can lead to age-related worsening. (iii) Clinical findings provide a basis for counselling patients and for designing therapeutic trials. Animal findings confirm a mouse model for investigation of underlying mechanisms of disease progression, and are also consistent with known mechanisms of ATP1A3-related neurodegeneration

The unsuccessful appeals of pope of Rome Leo XIII addressed to Abdul Hamid II to stop the massacres of the Armenians in the Ottoman empire (1894-1896)

The unsuccessful appeals of Pope of Rome Leo XIII addressed to Abdul Hamid II to stop the massacres of the Armenians in the Ottoman Empire (1894-1896) Vatican secret archive documents opened in recent years, which represent letters, reports, records and etc., have enormous historical and diplomatic value and importance in the context of history of the Armenian Genocide and Armenian Question. The archival vaults include eyewitness accounts and records of bishops, nuncios, missionaries of the Roman Catholic Church, as well as the representatives of the State Secretariat of the Vatican, and the priests of the Armenian Catholic and Grigoryan (Armenian Apostolic Church) Churches. In the archival vaults the interference documents of Leo XIII (1878-1903) and Benedict XV (1914-1922) Popes are distinguished with their value. The latters have attempted to intervene in the persecutions, deportations and massacres committed against Christians in the Ottoman Empire, although they were unsuccessful and ended with no result. In this context, it is noteworthy the intervene appeals of Pope Leo XIII addressed to Abdul Hamid II to stop the atrocities committed against Christians, which remain ignored by the sultan. Thus, the Vatican diplomatic intervention ends with failure

Armenian question in the super powers’ diplomacy agenda on the eve of the First World War: the reflection of the issue in the documents of the historical-diplomatic archives of the ministry of foreign affairs of Italy (1912/14)

The given article is devoted to the historical-diplomatic context of the Armenian Question on the eve of the First World War, when it was appeared in the Super Powers’ agenda. The term “Armenian Question”, as it is used in the history, became international among diplomatic circles and in the popular press after the Congress of Berlin in 1878. In the frames of the presented article, we have tried to analyze the reflection of that issue in the historical-diplomatic archival sources of the Ministry of Foreign Affairs of Italy. The detailed study of the archival documentations opens a new chapter on foreign policy of that time Super Powers discloses also the attitude and approaches of the Italian government towards Armenian issue. The detailed study and scientific formulation of the preserved archival documents of the Ministry of Foreign Affairs of Italy have political and diplomatic, and important historical significance from the perspective of the examined topic, that is, the Armenian Question

O OR RI IG GI IN NA AL L A AR RT TI IC CL LE E Effect of Green Manure and some Chemical Fertilizers on the Wheat Yield and some of its Components in Iran-North of Khuzestan

ABSTRACT In farm year of 2010-2011, a

Unerine fibrosis in young patients: clinical and pathogenetic

Detailed clinical and morphological study was undertaken on 101 young patients (age 20-30) diagnosed with Uterine fibrosis (the average patient age was 24,7±1,5 years). From the medical history, integrated clinical and morphological study, immunological analysis it was proved to exist two seldom forms of myomatous nodes at a young age: simplefibroid and invasive fibroid, that prevails. The growth originates in a vessel wall from the progenerating muscle cells with relatively high Ki-67 expression, high expression of growth factors and formation of new micro vessels (СD-34). These muscle cells have a very low apoptosis activity that determines the fast proliferation of cells (invasive fibroid)

SCIENTIFIC COOPERATION BETWEEN ARMENIAN AND THE EAEU: REALITY AND DEVELOPMENT TRENDS

The aim of this article is to analyze and assess the scientific collaboration between the Republic of Armenia (RA) and the Eurasian Economic Union (EAEU) member-states.In the Introduction, the article dwells into the integration processes that started right after the collapse of the Soviet Union with a main emphasis on the EAEU. The situation in the scientific sphere in Armenia is presented briefly, followed by highlighting the role of international scientific collaboration as a way to overcome a crisis in the science field.The role of the Committee of Sciences of the RA in the development of international collaboration is emphasized. The first steps in the framework of EAEU to activate scientific-technical cooperation are stressed.The Materials and Methods Section outlines the databases on which the study is based, that are the Web of Science Core Collection (WOS CC) and the Russian Science Citation Index (RSCI). The timeframe for the data retrieved from the WOS CC is 1991–2016, while for the RSCI — 2005–2016. The types of collaborations, as well as five stages of research — data retrieval from the WOS CC and the RSCI, data cleaning, quantitative analysis of citation, quantitative analysis of co-authored papers of the RA with each EAEU member-state and the distribution of the co-authored papers by the scientific fields — are mentioned.The Results Section presents the results of the study by the five mentioned stages and the relevant analysis.The Discussion and Conclusion Section presents the main conclusions of the study. It mainly stresses that Russia is the main scientific partner of Armenia, followed by Belarus. Scientific collaboration of Armenia with Kazakhstan and Kirgizia are on a very low level. Physics was recognized to be the leading field of collaboration. Generally, the article concludes that it is too early to speak about the role of the EAEU in the development of scientific collaboration between the member-states. In order to assure a stable growth of collaboration among the EAEU member-states a huge work should be done