Cross-cultural validation of the Health Care Provider HIV/AIDS Stigma Scale (HPASS) in China

The study aimed to validate the Health Care Provider HIV/AIDS Stigma Scale among medical staff in China. The validation was conducted in four steps from March to December 2017: translation and back-translation; content validity test with six experts; test–retest reliability testing with 63 medical staff with 2 weeks interval; and structural validation with 349 medical staff from 52 hospitals with a convenience sample,using exploratory factor analysis,including principal component analysis and varimax rotation. The scale content validity index average was 0.88, while for test–retest reliability, the ICC was 0.87. Three factors of “discrimination”, “prejudice” and “stereotype” with 16 items were extracted and explained 59.61% variance. The Cronbach’s alpha value for the total scale was of 0.88, and for the three factors, the values were 0.89, 0.86 and 0.74, respectively. The discrimination factor showed identical means between Canadian medical students and Chinese medical staff, while the prejudice and stereotype factors had higher mean scores in the Chinese sample. The three-factor structure of Health Care Provider HIV/AIDS Stigma Scale was confirmed in Chinese medical staff with a simpler solution. This could provide a basis for trans-cultural application and comparison

Correlation between preconception maternal non-occupational exposure to interior decoration or oil paint odour and average birth weight of neonates: findings from a nationwide cohort study in China\u27s rural areas

BACKGROUND: Birth weight is a critical indicator of neonatal health and foretells people\u27s health in adolescence and even adulthood. Some researchers have warned against the adverse effects on babies\u27 birth weight of exposure to pollutants in interior decoration or oil paint by odour intake. This study evaluated the effects of maternal exposure to such factors before conception on the birth weights of neonates. METHODS: Data on 213 461 cases in this study were from the database of the free National Pre-pregnancy Checkups Project. Defined as \u27exposed\u27 were those women exposed to oil paint odour or interior decoration at home or in the workplace within 6 months before their pregnancy. The study focused on revealing the correlation between such exposure and the birth weight of the neonates of these women, especially the incidence of macrosomia and low birth weight (LBW). Statistical analysis was conducted using the Kruskal-Wallis H test, the Mann-Whitney U test and logistic regression. RESULTS: The birth weight of babies from mothers non-occupationally exposed to such settings averaged 3465 g (range 3150-3650 g), whereas the birth weight of those from mothers free of such exposure averaged 3300 g (range 3000-3600g). Maternal exposure preconception to interior decoration or oil paint odour reduced the incidence of LBW in their babies (p=0.003, OR 0.749, 95% CI 0.617 to 0.909). Such exposure may also augment the probability of macrosomia (p \u3c 0.001, OR 1.297, 95% CI 1.133 to 1.484). CONCLUSION: Maternal exposure to interior decoration or oil paint odour preconception may increase the average birth weight of neonates, as well as the incidence of macrosomia

Effect of Wu Zhi San supplementation in LPS-induced intestinal inflammation and barrier damage in broilers

Intestinal inflammation and barrier damage can inhibit the absorption and transportation of nutrients in the small intestine, and lead to various chronic diseases. Wu Zhi San (WZS) is a traditional Chinese formula composed of Schisandrae, Anemarrhenae, Lonicerae, and Glycyrrhizae that was made to cure intestinal inflammation and barrier damage in broilers. To evaluate the protective effect of WZS on intestinal inflammation and barrier damage of broilers under lipopolysaccharide (LPS) stress, a total of 200 one-day-old broilers were randomly divided into five groups, namely, the CON group, LPS group, and three WZS groups (WZS-H, WZS-M, and WZS-L). The groups were designed for stress phase I (days 15, 17, 19, and 21) and stress phase II (days 29, 31, 33, and 35). The protective effect of WZS on the intestinal tract was evaluated by measuring the levels of serum myeloperoxidase (MPO), diamine oxidase (DAO), super oxide dismutase (SOD), and serum D-lactate (D-LA) and the expression of inflammatory factors in jejunum. The results showed that the diet supplemented with WZS could significantly reduce serum MPO, DAO, and D-LA levels and jejunal CD in broilers (p < 0.05), increase serum SOD levels and jejunal VH (p < 0.05), significantly downregulate the expression of NF-κB, TLR4, MyD88, and inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-10), and upregulate Claudin-1, Occludin-1, and ZO-1 in broiler jejunum mucosa (p < 0.05). On the other hand, WZS could significantly reduce the protein expression of NF-κB (p65) in broiler jejunum (p < 0.05). These results indicate that supplementing WZS in the diet can reduce intestinal inflammation and alleviate intestinal barrier damage, and by inhibiting the NF-κB/TLR4/MyD88 signaling pathway, supplementation with WZS intervenes in LPS-induced stress injury in broilers

The Acute Liver Injury in Mice Caused by Nano-Anatase TiO2

Although it is known that nano-TiO2or other nanoparticles can induce liver toxicities, the mechanisms and the molecular pathogenesis are still unclear. In this study, nano-anatase TiO2(5 nm) was injected into the abdominal cavity of ICR mice for consecutive 14 days, and the inflammatory responses of liver of mice was investigated. The results showed the obvious titanium accumulation in liver DNA, histopathological changes and hepatocytes apoptosis of mice liver, and the liver function damaged by higher doses nano-anatase TiO2. The real-time quantitative RT-PCR and ELISA analyses showed that nano-anatase TiO2can significantly alter the mRNA and protein expressions of several inflammatory cytokines, including nucleic factor-κB, macrophage migration inhibitory factor, tumor necrosis factor-α, interleukin-6, interleukin-1β, cross-reaction protein, interleukin-4, and interleukin-10. Our results also implied that the inflammatory responses and liver injury may be involved in nano-anatase TiO2-induced liver toxicity

Gu-4 Suppresses Affinity and Avidity Modulation of CD11b and Improves the Outcome of Mice with Endotoxemia and Sepsis

BACKGROUND: Systemic leukocyte activation and disseminated leukocyte adhesion will impair the microcirculation and cause severe decrements in tissue perfusion and organ function in the process of severe sepsis. Gu-4, a lactosyl derivative, could selectively target CD11b to exert therapeutic effect in a rat model of severe burn shock. Here, we addressed whether Gu-4 could render protective effects on septic animals. METHODOLOGY/PRINCIPAL FINDINGS: On a murine model of endotoxemia induced by lipopolysaccharide (LPS), we found that the median effective dose (ED50) of Gu-4 was 0.929 mg/kg. In vivo treatment of Gu-4 after LPS challenge prominently attenuated LPS-induced lung injury and decreased lactic acid level in lung tissue. Using the ED50 of Gu-4, we also demonstrated that Gu-4 treatment significantly improved the survival rate of animals underwent sepsis induced by cecal ligation and puncture. By adhesion and transwell migration assays, we found that Gu-4 treatment inhibited the adhesion and transendothelial migration of LPS-stimulated THP-1 cells. By flow cytometry and microscopy, we demonstrated that Gu-4 treatment inhibited the exposure of active I-domain and the cluster formation of CD11b on the LPS-stimulated polymorphonuclear leukocytes. Western blot analyses further revealed that Gu-4 treatment markedly inhibited the activation of spleen tyrosine kinase in LPS-stimulated THP-1 cells. CONCLUSIONS/SIGNIFICANCE: Gu-4 improves the survival of mice underwent endotoxemia and sepsis, our in vitro investigations indicate that the possible underlying mechanism might involve the modulations of the affinity and avidity of CD11b on the leukocyte. Our findings shed light on the potential use of Gu-4, an interacting compound to CD11b, in the treatment of sepsis and septic shock

Evaluation of river connectivity using a composite index method and its impact on nutrients dynamics in large rivers

The connectivity of large rivers is impaired by human activities, which could cause severe consequences to hydrodynamics, water quality. To clarify the changes in hydrological connectivity of large rivers and their effects on nutrients, a composite index method of river connectivity in longitudinal, lateral and vertical dimensions was developed in this study. Taking the middle reaches of the Yangtze River as an example, the hydrological connectivity of typical hydrological years and periods in the region was analyzed, and the responses of critical nutrient indexes to varied river connectivity was assessed. The results showed that significant temporal and spatial variations in river connectivity were observed, with better river connectivity in the wet year than the dry year, and worse connectivity in headwaters, tributaries, or downstream of gates and dams than other river reaches. This was mainly due to the degree of river fragmentation (DOF) and the degree of regulation (DOR) with a relative importance of 81.28% and 56.07%, respectively, implying that gate and dam interception was the main cause of impaired river connectivity in the study area. Statistical methods indicated that the impaired connectivity in the middle reaches of the Yangtze River implied a stronger retention of nutrients by dams, and that retention effect was greater during the flood period than the non-flood period. The stronger negative correlation between connectivity and TP (correlation coefficient = −0.58) than TN (correlation coefficient = −0.46) during the flood period revealed that phosphorus was more influenced by river connectivity. The results of this study provide important information on water resources and water quality for the management of large rivers

Interrelations of Ecosystem Services and Rural Population Wellbeing in an Ecologically-Fragile Area in North China

Ecosystem services (ES) are indispensable contributors to rural population wellbeing (RPWB). Understanding the relationship between ES and RPWB is important to human welfare and conserving ecosystem services. Ecologically-vulnerable areas are widely distributed in China and are concentrated with impoverished people. This study explored the relations of ES and RPWB in an ecologically-fragile area, and provided some recommendations for regional improvement. We developed an evaluation framework, analyzed the spatio-temporal characteristics of services and RPWB, and examined their relations. Results showed that: (1) the level of RPWB in an ecologically-vulnerable area was lower than that in the downstream region; however, the wellbeing gap between regions has narrowed over time; (2) Regions with a higher ecosystem service capacity had higher levels of rural population wellbeing, and vice versa; (3) In addition to the food supply, other ES indicators were closely associated with RPWB in the region. Specifically, the net income and living space were closely related to food supply, while rural-urban income ratio and water environment were more relevant to the other services. Lastly, it is important to address environment and poverty issues simultaneously