The efficient expression of nattokinase in Escherichia coli by sequence optimization

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Inhibition of TRPA1 Attenuates Doxorubicin-Induced Acute Cardiotoxicity by Suppressing Oxidative Stress, the Inflammatory Response, and Endoplasmic Reticulum Stress

The transient receptor potential ankyrin 1 (TRPA1) channel is expressed in cardiomyocytes and involved in many cardiovascular diseases. However, the expression and function of TRPA1 in doxorubicin- (Dox-) induced acute cardiotoxicity have not been elucidated. This study aimed at investigating whether blocking the TRPA1 channel with the specific inhibitor HC-030031 (HC) attenuates Dox-induced cardiac injury. The animals were randomly divided into four groups: control, HC, Dox, and Dox + HC. Echocardiography was used to evaluate cardiac function, and the heart was removed for molecular experiments. The results showed that the expression of TRPA1 was increased in the heart after Dox treatment. Cardiac dysfunction and increased serum CK-MB and LDH levels were induced by Dox, but these effects were attenuated by HC treatment. In addition, HC mitigated Dox-induced oxidative stress, as evidenced by the decreased MDA level and increased GSH level and SOD activity in the Dox + HC group. Meanwhile, HC treatment lowered the levels of the proinflammatory cytokines IL-1β, IL-6, IL-17, and TNF-α induced by Dox. Furthermore, HC treatment mitigated endoplasmic reticulum (ER) stress and cardiomyocyte apoptosis induced by Dox. These results indicated that inhibition of TRPA1 could prevent Dox-induced cardiomyocyte apoptosis in mice by inhibiting oxidative stress, inflammation, and ER stress

Effect of Low Temperature Vacuum Slow Cooking on Muscle Quality Characteristics of Half Shell Scallop

This study utilized half-shell Chlamys farreri as the primary material to investigate the effects of different sous vide (SV) cooking temperatures (60, 65, 70, 75, 80 ℃) and cooking times (30, 45, 60, 75 min) on the quality attributes of the Chlamys farreri muscle. Sensory quality, drip loss, moisture content, water activity, texture, myofibrillar protein extraction, and microstructure were evaluated as key indicators. The results showed that as the cooking temperature and time increased, the sensory scores of SV Chlamys farreri initially rose and then declined, with the highest sensory score observed at 70 and 75 ℃ after 30 minutes of cooking. The drip loss of SV Chlamys farreri increased between 60 and 75 ℃ without significant differences (P0.05). Scanning electron microscopy and HE staining illustrated that as the cooking temperature increased, myofibrils underwent breakage, boundaries became indistinct, fractured myofibrils were cross-linked, and the disorder level escalated, ultimately leading to the aggregation of broken myofibrils into large masses. In conclusion, the optimal sensory quality of the scallops was achieved at 70 and 75 ℃ with 30 minutes of SV cooking, where drip loss and elasticity were lower than those in the control group (100 ℃, 15 min), while moisture content, water activity, hardness, and myofibrillar protein extraction rate were higher than those in the control group

A methodology of integrating affective design with defining engineering specifications for product design

Affective design and the determination of engineering specifications are commonly conducted separately in early product design stage. Generally, designers and engineers are required to determine the settings of design attributes (for affective design) and engineering requirements (for engineering design), respectively, for new products. Some design attributes and some engineering requirements could be common. However, the settings of the design attributes and engineering requirements could be different because of the separation of the two processes. In previous studies, a methodology that considers the determination of the settings of the design attributes and engineering requirements simultaneously was not found. To bridge this gap, a methodology for considering affective design and the determination of engineering specifications of a new product simultaneously is proposed. The proposed methodology mainly involves generation of customer satisfaction models, formulation of a multi-objective optimisation model and its solving using a chaos-based NSGA-II. To illustrate and validate the proposed methodology, a case study of mobile phone design was conducted. A validation test was conducted and the test results showed that the customer satisfaction values obtained based on the proposed methodology were higher than those obtained based on the combined standalone quality function deployment and standalone affective design approach

Rough set and PSO-based ANFIS approaches to modeling customer satisfaction for affective product design

Facing fierce competition in marketplaces, companies try to determine the optimal settings of design attribute of new products from which the best customer satisfaction can be obtained. To determine the settings, customer satisfaction models relating affective responses of customers to design attributes have to be first developed. Adaptive neuro-fuzzy inference systems (ANFIS) was attempted in previous research and shown to be an effective approach to address the fuzziness of survey data and nonlinearity in modeling customer satisfaction for affective design. However, ANFIS is incapable of modeling the relationships that involve a number of inputs which may cause the failure of the training process of ANFIS and lead to the 'out of memory' error. To overcome the limitation, in this paper, rough set (RS) and particle swarm optimization (PSO) based-ANFIS approaches are proposed to model customer satisfaction for affective design and further improve the modeling accuracy. In the approaches, the RS theory is adopted to extract significant design attributes as the inputs of ANFIS and PSO is employed to determine the parameter settings of an ANFIS from which explicit customer satisfaction models with better modeling accuracy can be generated. A case study of affective design of mobile phones is used to illustrate the proposed approaches. The modeling results based on the proposed approaches are compared with those based on ANFIS, fuzzy least-squares regression (FLSR), fuzzy regression (FR), and genetic programming-based fuzzy regression (GP-FR). Results of the training and validation tests show that the proposed approaches perform better than the others in terms of training and validation errors.School of DesignDepartment of Industrial and Systems Engineerin

The effects of anesthetics on recurrence and metastasis of cancer, and clinical implications

Surgical resection of the primary tumor may enhance the metastasis and recurrence of cancer. The reaction of patients to surgery includes changes of the immune system, the inflammatory system and the neuroendocrine system. In the perioperative period, anesthetics are used both for anesthesia and analgesia. There are several studies showing that the progression of cancer can be influenced by many kinds of anesthetics, although most of these studies are preclinical and thus have not yet influenced clinical recommendations. This review summarizes recent studies regarding the effects of anesthetics on metastasis and recurrence of cancer

A three-dimensional actively spreading bone repair material based on cell spheroids can facilitate the preservation of tooth extraction sockets

Introduction: Achieving a successful reconstruction of alveolar bone morphology still remains a challenge because of the irregularity and complex microenvironment of tooth sockets. Biological materials including hydroxyapatite and collagen, are used for alveolar ridge preservation. However, the healing effect is often unsatisfactory.Methods: Inspired by superwetting biomimetic materials, we constructed a 3D actively-spreading bone repair material. It consisted of photocurable polyether F127 diacrylate hydrogel loaded with mixed spheroids of mesenchymal stem cells (MSCs) and vascular endothelial cells (ECs).Results: Biologically, cells in the spheroids were able to spread and migrate outwards, and possessed both osteogenic and angiogenic potential. Meanwhile, ECs also enhanced osteogenic differentiation of MSCs. Mechanically, the excellent physical properties of F127DA hydrogel ensured that it was able to be injected directly into the tooth socket and stabilized after light curing. In vivo experiments showed that MSC-EC-F127DA system promoted bone repair and preserved the shape of alveolar ridge within a short time duration.Discussion: In conclusion, the novel photocurable injectable MSC-EC-F127DA hydrogel system was able to achieve three-dimensional tissue infiltration, and exhibited much therapeutic potential for complex oral bone defects in the future

Room-temperature ferromagnetism in epitaxial bilayer FeSb/SrTiO3(001) terminated with a Kagome lattice

Two-dimensional (2D) magnets exhibit unique physical properties for potential applications in spintronics. To date, most 2D ferromagnets are obtained by mechanical exfoliation of bulk materials with van der Waals interlayer interactions, and the synthesis of single or few-layer 2D ferromagnets with strong interlayer coupling remains experimentally challenging. Here, we report the epitaxial growth of 2D non-van der Waals ferromagnetic bilayer FeSb on SrTiO3(001) substrates stabilized by strong coupling to the substrate, which exhibits in-plane magnetic anisotropy and a Curie temperature above 300 K. In-situ low-temperature scanning tunneling microscopy/spectroscopy and density-functional theory calculations further reveal that a Fe Kagome layer terminates the bilayer FeSb. Our results open a new avenue for further exploring emergent quantum phenomena from the interplay of ferromagnetism and topology for application in spintronics

Aberration in DNA Methylation in B-Cell Lymphomas Has a Complex Origin and Increases with Disease Severity

Despite mounting evidence that epigenetic abnormalities play a key role in cancer biology, their contributions to the malignant phenotype remain poorly understood. Here we studied genome-wide DNA methylation in normal B-cell populations and subtypes of B-cell non-Hodgkin lymphoma: follicular lymphoma and diffuse large B-cell lymphomas. These lymphomas display striking and progressive intra-tumor heterogeneity and also inter-patient heterogeneity in their cytosine methylation patterns. Epigenetic heterogeneity is initiated in normal germinal center B-cells, increases markedly with disease aggressiveness, and is associated with unfavorable clinical outcome. Moreover, patterns of abnormal methylation vary depending upon chromosomal regions, gene density and the status of neighboring genes. DNA methylation abnormalities arise via two distinct processes: i) lymphomagenic transcriptional regulators perturb promoter DNA methylation in a target gene-specific manner, and ii) aberrant epigenetic states tend to spread to neighboring promoters in the absence of CTCF insulator binding sites