Transcriptome analysis of epithelioma papulosum cyprini cells after SVCV infection

BACKGROUND: Spring viraemia of carp virus (SVCV) has been identified as the causative agent of spring viraemia of carp (SVC) and it has caused significant losses in the cultured common carp (Cyprinus carpio) industry. The molecular mechanisms that underlie the pathogenesis of the disease remain poorly understood. In this study, deep RNA sequencing was used to analyse the transcriptome and gene expression profile of EPC cells at progressive times after SVCV infection. This study addressed the complexity of virus–cell interactions and added knowledge that may help to understand SVCV. RESULTS: A total of 33,849,764 clean data from 36,000,000 sequence reads, with a mean read length 100 bp, were obtained. These raw data were assembled into 88,772 contigs. Of these contigs, 19,642 and 25,966 had significant hits to the NR and Uniprot databases where they matched 17,642 and 13,351 unique protein accessions, respectively. At 24 h post SVCV infection (1.0 MOI), a total of 623 genes were differentially expressed in EPC cells compared to non-infected cells, including 288 up-regulated genes and 335 down-regulated genes. These regulated genes were primarily involved in pathways of apoptosis, oxidative stress and the interferon system, all of which may be involved in viral pathogenesis. In addition, 8 differentially expressed genes (DEGs) were validated by quantitative PCR. CONCLUSIONS: Our findings demonstrate previously unrecognised changes in gene transcription that are associated with SVCV infection in vitro, and many potential cascades identified in the study clearly warrant further experimental investigation. Our data provide new clues to the mechanism of viral susceptibility in EPC cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-935) contains supplementary material, which is available to authorized users

Effect of pre-homogenizing treatment on microstructure and mechanical properties of hot-rolled AZ91 magnesium alloys

To improve the homogeneity and rolling formability of as-cast AZ91 magnesium, the effects of pre-homogenizing treatment on microstructure evolution, deformation mechanism, mechanical properties and tensile fracture morphology of hot-rolled AZ91 magnesium alloy were studied. The results showed that the amount of coarse β-Mg17Al12 phase decreases dramatically, being distributed along the grain boundaries as small strips after homogenizing. Twining plays a dominant role in the deformation mechanism of AZ91 alloys in the experimental condition, while dynamic recrystallization (DRX) considerably occurred in homogenized-rolled alloys, contributed to microstructure uniformity and β-Mg17Al12 phase precipitated refinement. The tensile strength of homogenized-rolled AZ91 alloys increases dramatically with elongation declining slightly in contrast to homogenized alloys. The fracture surface of homogenized-rolled specimen exhibits more ductile fracture with the manifestation of a large amount of dimples distributing higher density in matrix, while the micro cracks are prone to initiate around the Mg/Mg17Al12 phase interface and grain boundaries owing to the fragile interface bonding of two phases

Influence of equivalent stiffness form from stern bearing to transverse vibration of shafting

ObjectivesDue to the larger length-to-diameter ratio of the stern bearing, it is difficult to reflect its actual operating conditions when simplified to the traditional equivalent model of single-point support. Therefore, the influence of the equivalent form of the stern bearing on the transverse vibration characteristics of the shafting is investigated.MethodsThe improved Fourier series is introduced to describe the lateral vibration displacement of propulsion shafting. Then, the calculation model of lateral vibration performance of propulsion shafting under various equivalent forms, such as single-point support, multi-point support or continuous distributed support, are constructed based on the energy principle. Thereby, the influence of the change of support stiffness equivalent to the liquid film pressure on the lateral vibration of the shafting and the influence of the propeller excitation on the vibration response of the shafting are further analyzed. Finally, the results acquired by the proposed model is compared with the results of related references and finite element method (FEM) to verify the validity of the calculation model.ResultsThe multi-point support calculation results converge to the continuous distributed support calculation results. The three-point support equivalent form can be used to study the influence of liquid film pressure distribution on the lateral vibration characteristics of the propulsion shafting. The shafting response under propeller excitation is affected by the revolution speed.Conclusions The research indicates that three-point support equivalent form can be used to analyze the influence of liquid film pressure on the shafting lateral vibration performance. The proposed model in this paper has advantages of good convergence, high accuracy, and less cost-consuming

Binding of Cd(II) by Amorphous Aluminum Hydroxide-Organophosphorus Coprecipitates: From Macroscopic to Microscopic Investigation

The mobility of Cd(II) in soils, sediments, and aquatic systems is strongly dependent on adsorption behaviors occurring at the mineral-water interface, and this process may be influenced by the presence of organic phosphorus (OP). In this study, we investigate Cd(II) adsorption onto amorphous aluminum hydroxide (AAH), both in the presence and absence of OP, represented by the widely abundant myo-inositol hexakisphosphate (IHP). Isothermal adsorption experiment coupled with attenuated total reflection Fourier transform infrared (ATR-FTIR) and 1H solid-state NMR spectra were employed. Physiochemical characterization shows that IHP can increase the surface negative charge and the number of surface sites. Isothermal results show that high IHP loading enhances Cd(II) adsorption while no obvious increase is observed at low IHP loading. The overall effect of IHP on Cd(II) sorption depends on the extent of two positive processes, i.e., (1) IHP can form ternary complexes with adsorbed Cd(II) on AAH and (2) IHP can increase the negative surface charge of AAH, and a negative process, i.e., AAH competes with Cd(II) for AAH surface sites. ATR-FTIR results confirm the possible formation of three structurally distinct ternary complexes, i.e., the AAH-IHP-Cd, AAH-Cd-IHP, and AAH-Cd-IHP-Cd. The analysis of 1H solid-state NMR demonstrates that IHP only increases the number of surface OH groups rather than changes their chemical environment and speciation. Cd does not bind to the AAH surface but mainly binds with the OH groups of IHP. All findings of this work suggest that the presence of high dose of OP promotes the retention of Cd(II) in soils, thereby decreasing their bioavailability to biota

CS-FCDA: A Compressed Sensing-Based on Fault-Tolerant Data Aggregation in Sensor Networks

When the nodes in the network are deployed in the target area with an appropriate density, the effective aggregation and transmission of the data gathered in the monitoring area remain to be solved. The existing Compressed Sensing (CS) based on data aggregation schemes are accomplished in a centralized manner and the Sink node achieves the task of data aggregation. However, these existing schemes may suffer from load imbalance and coverage void issues. In order to address these problems, we propose a Compressed Sensing based on Fault-tolerant Correcting Data Aggregation (CS-FCDA) scheme to accurately reconstruct the compressed data. Therefore, the network communication overhead can be greatly reduced while maintaining the quality of the reconstructed data. Meanwhile, we adopt the node clustering mechanism to optimize and balance the network load. It is shown via simulation results, compared with other data aggregation schemes, that the proposed scheme shows obvious improvement in terms of the Fault-tolerant correcting capability and the network energy efficiency of the data reconstruction