Population Density of Grey Francolin (Franclinus Pondicrianus L.) in District Tando Allahyar, Sindh, Pakistan

The population density of Grey Francolin (Franclinus Pondicrianus L.) is distributed throughout the Baluchistan, KKPK, Punjab, and Indus plains of Sindh Province, Pakistan. The grey francolin is a prime game bird of our country. This species has been declared threatened worldwide according to the Red Data Book, also published by the International Union for Conservation of Nature in 2018. There is no information available regarding the density of the population of Grey francolin in different populations in the district of Tando Allahyar, Sindh, Pakistan. Keeping this in mind, the present study was carried out to find the density population of Grey francolin birds in the habitat area to observe the conservation measurements. The study was conducted through direct sighting with the help of local residents of the particular areas using the Visual Encounter Method. The observations were recorded at three fixed transects, with a length of 300 to 350 m and a width of 30 to 60 m laid down at every site for recording the birds by nearest line. In Tando Allahyar, Sindh, Pakistan, the population density of Grey francolin varies significantly based on breeding practices and habitat characteristics. The observed densities were 0.90 birds per hectare in cultivated open land and 0.16 birds per hectare in wetland and associated natural vegetation. This suggests a notable influence of habitat and breeding practices on Grey francolin population

IMMUNOLOCALIZATION OF THE SMAD4 PROTEIN IN POSTNATAL PORCINE UTERUS

ABSTRACT Transforming growth factor beta (TGF-β) regulates a series of effects on biological events, including uterine morphogenesis. SMAD4, downstream signal protein of the TGF superfamily, is an essential factor for mediating the TGF-β superfamily signaling. In the current study, our objective was to investigate the localization and expression of SMAD4 in postnatal porcine uterus using immunohistochemistry to provide experimental clues illustrating the mechanism of TGF-β superfamily signaling during the process of uterine development. Our results revealed that SMAD4 was detected among all samples examined, and widely and differentially expressed in the uterine luminal and glandular epithelium, myometrium and stroma in different age groups. Our findings strongly suggested that TGF-β superfamily signaling may be involved in postnatal uterine development in pigs

Oviduct-specific expression of tissue plasminogen activator in laying hens

Egg-laying hens are important candidate bioreactors for pharmaceutical protein production because of the amenability of their eggs for protein expression. In this study, we constructed an oviduct-specific vector containing tissue plasminogen activator (tPA) protein and green fluorescent protein (pL-2.8OVtPAGFP) and assessed its expression in vitro and in vivo. Oviduct epithelial and 3T3 cells were cultured and transfected with pL-2.8OVtPAGFP and pEGP-N1 (control vector), respectively. The pL-2.8OVtPAGFP vector was administered to laying hens via a wing vein and their eggs and tissues were examined for tPA expression. The oviduct-specific vector pL-2.8OVtPAGFP was expressed only in oviduct epithelial cells whereas pEGP-N1 was detected in oviduct epithelial and 3T3 cells. Western blotting detected a 89 kDa band corresponding to tPA in egg white and oviduct epithelial cells, thus confirming expression of the protein. The amount of tPAGFP in eggs ranged 9 to 41 ng/mL on the third day after vector injection. The tPA expressed in egg white and oviduct epithelial cells showed fibrinolytic activity, indicating that the protein was expressed in active form. GFP was observed only in oviducts, with no detection in heart, muscle, liver and intestine. This is the first study to report the expression of tPA in egg white and oviduct epithelial cells using an oviduct-specific vector

Heritability Estimates for some Performances Traits of Baluchi Sheep

Present study was carried out to estimates the genetic parameters of Baluchi sheep during the year 2015 at Bhagnari cattle Cum Baluchi Sheep Farm Usta Muhammad, Baluchistan. The recorded data including (lactation yield and lactation length) was collected for the period 2005 to 2014.The results of current study revealed that average milk yield and lactation length was found 95.1±11.122kg and 123.60±8.44days of Baluchi sheep. The results for heritability and correlation estimates for lactation yield and lactation length was observed 0.113, 0.126 and 0.26, respectively. There was positive and low heritability and correlation was worked out for lactation yield and lactation length. Due to low results heritability and correlation estimates of some performance traits of Baluchi sheep, it was concluded that improvement can be achieved by process of mass selection

Heritability Estimates for some Performances Traits of Baluchi Sheep

Present study was carried out to estimates the genetic parameters of Baluchi sheep during the year 2015 at Bhagnari cattle Cum Baluchi Sheep Farm Usta Muhammad, Baluchistan. The recorded data including (lactation yield and lactation length) was collected for the period 2005 to 2014.The results of current study revealed that average milk yield and lactation length was found 95.1±11.122kg and 123.60±8.44days of Baluchi sheep. The results for heritability and correlation estimates for lactation yield and lactation length was observed 0.113, 0.126 and 0.26, respectively. There was positive and low heritability and correlation was worked out for lactation yield and lactation length. Due to low results heritability and correlation estimates of some performance traits of Baluchi sheep, it was concluded that improvement can be achieved by process of mass selection

Effect of Dextrose Sugar on the Growth and Production of Oyster Mushroom (Pleurotus ostreatus) through Tissue Culture

The study was conducted to investigate the dextrose sugar effect as carbon source on mycelial growth and production of Oyster mushroom (Pleurotus ostreatus). The experiment was performed in Mushroom Laboratory, Plant Pathology Section, Agriculture Research Institute, Tandojam, during 2013-2014. Mycelial growth was developed by using tissue culture on medium (PDA) potato dextrose agar with various concentrations of dextrose sugar. Analysis of variance for concentrations was statistically highly significant for all the parameters. In some cases among the different concentrations, 2.0% dextrose sugar showed after 2 days of micro propagation, the mycelial growth (1.9 cm) was recorded, followed by 1.5% dextrose sugar that showed (1.7 cm). The earlier spawn mycelia growth was observed in case of amending same 0/2% dextrose sugar (24.5 days). The pinhead first appeared (29.5 days) after the date of spawning by using 2.0% dextrose sugar. The minimum period (4.2 days) for maturation of mushroom fruiting body were recorded at 20% and 1.5% dextrose sugar. The maximum numbers of fruiting bodies (56.2) were observed with an application of dextrose sugar at 2.0%. The maximum number of bunches per bag (16.5) were harvested with an application of dextrose sugar 2.0%. The highest (350.5 g) fresh yield of Oyster mushroom Pleurotus ostreatus was recorded from 2.0% am ended of dextrose sugar

Effect of Dextrose Sugar on the Growth and Production of Oyster Mushroom (Pleurotus ostreatus) through Tissue Culture

The study was conducted to investigate the dextrose sugar effect as carbon source on mycelial growth and production of Oyster mushroom (Pleurotus ostreatus). The experiment was performed in Mushroom Laboratory, Plant Pathology Section, Agriculture Research Institute, Tandojam, during 2013-2014. Mycelial growth was developed by using tissue culture on medium (PDA) potato dextrose agar with various concentrations of dextrose sugar. Analysis of variance for concentrations was statistically highly significant for all the parameters. In some cases among the different concentrations, 2.0% dextrose sugar showed after 2 days of micro propagation, the mycelial growth (1.9 cm) was recorded, followed by 1.5% dextrose sugar that showed (1.7 cm). The earlier spawn mycelia growth was observed in case of amending same 0/2% dextrose sugar (24.5 days). The pinhead first appeared (29.5 days) after the date of spawning by using 2.0% dextrose sugar. The minimum period (4.2 days) for maturation of mushroom fruiting body were recorded at 20% and 1.5% dextrose sugar. The maximum numbers of fruiting bodies (56.2) were observed with an application of dextrose sugar at 2.0%. The maximum number of bunches per bag (16.5) were harvested with an application of dextrose sugar 2.0%. The highest (350.5 g) fresh yield of Oyster mushroom Pleurotus ostreatus was recorded from 2.0% am ended of dextrose sugar

Correlation Estimates between Carcass Traits of Nili Ravi and Kundhi Buffalo

Present study was designed to estimates the correlation between carcass traits of Nili Ravi and Kundhi buffalo. The data for carcass traits of Nili Ravi and Kundhi buffalo was collected from Seven Star International Meat Processing Company Dhabeji at Thatta. In current study the data of total 100 animals of Kundhi and Nili Ravi breed were selected and divided into A, B, C and D group. In group A and C there were Kundhi and Nili Ravi male whereas, B and D females of both breeds respectively. The data including live body weight, carcass weight, dressing percentage and boneless weight of both breeds Kundhi and Nili Ravi were collected for the estimation of correlation.The results for correlation estimates of different carcass traits indicated that the correlation estimation were found positive and high among Nili Rave breed as compared to Kundhi breed, which shows that an increase in one carcass trait would increase the other carcass traits. It was concluded that Nili Ravi carcass traits are better expressed and produces more beef than Kundhi, while Kundhi male is better in beef production than the Nili Ravi female whereas Kundhi female produces low carcass yield

Correlation Estimates between Carcass Traits of Nili Ravi and Kundhi Buffalo

Present study was designed to estimates the correlation between carcass traits of Nili Ravi and Kundhi buffalo. The data for carcass traits of Nili Ravi and Kundhi buffalo was collected from Seven Star International Meat Processing Company Dhabeji at Thatta. In current study the data of total 100 animals of Kundhi and Nili Ravi breed were selected and divided into A, B, C and D group. In group A and C there were Kundhi and Nili Ravi male whereas, B and D females of both breeds respectively. The data including live body weight, carcass weight, dressing percentage and boneless weight of both breeds Kundhi and Nili Ravi were collected for the estimation of correlation.The results for correlation estimates of different carcass traits indicated that the correlation estimation were found positive and high among Nili Rave breed as compared to Kundhi breed, which shows that an increase in one carcass trait would increase the other carcass traits. It was concluded that Nili Ravi carcass traits are better expressed and produces more beef than Kundhi, while Kundhi male is better in beef production than the Nili Ravi female whereas Kundhi female produces low carcass yield

Effect of Genetic Parameters on Some Growth Performance Traits of Harnai Sheep

Present study was performed to estimates the genetic parameters forsome growth performance traits of Harnai sheep. The data was recorded for the period of 2004-2013 from the Multi-purpose research centre Yetabad, District, Baluchistan. The performance traits including birth, yearling, weaning and fleece weight was recorded for the estimation of genetic parameters. There was no significant difference was observed parity and Ram wise among some growth performance traits of Harnai sheep. While the results for heritability, estimation for birth weight, yearling weight, weaning weight and fleece weight was observed low to medium for some growth performance traits of Harnai sheep. It is concluded that low heritable and correlative traits mainly affected by the management, nutritional and temporary environmental conditions, hence improvement can be achieved through the better selection