Thinking about Mode of Explosive Products

The development of businesses in the Internet era has shifted from channel to scale, to users and word of mouth. By utilizing the fans economy, the mode of explosive products has become an effective mode for focusing on core products, efficiently detonating the market, quickly acquiring users, and realizing enterprise development. This paper conducts pertinent analysis and research. It discusses how to distinguish between explosive products, explosive products, and explosive products, as well as the relevant capabilities required to build explosive products. Finally, in conjunction with the current situation, it discusses how to establish a mode of explosive products for enterprises in order to realize enterprise growth and profit

Do reviews from friends and the crowd affect online consumer posting behaviour differently?

User-generated reviews are valuable resources for consumers to gain information of products which has significant impact on their following decision-making. With the development of social network service, consumers are exposed to reviews coming from both friends and the crowds (non-friends). However, the impact of friends’ and crowds’ reviews on consumer posting behaviour has not been well differentiated. Using the online review information as well as the underlying social network from Yelp, this paper develops a multilevel mixed effect probit model to study the impact of consumer characteristics and reviews of different sources, i.e. friends or crowds, on the possibility of consumer further engaging in posting behaviour. Despite the common perception that the volume, valance and variance of reviews significantly impact the possibility of following posting behaviour, we show that such influence majorly comes from the friend reviews. The volume of friend reviews has much stronger impact on the target user’s posting behaviour than that of the crowds. The valance and variance of the crowd reviews show no significant influence when ignoring the friend reviews, but negative influence when considering it. The friend reviews and crowd reviews are further divided as positive and negative ones, and only the positive friend reviews and negative crowd review are found significantly enhancing the posting possibility

Evaluation of translocation impacts on genetic patterns in farmed and naturalized populations of Mytilus galloprovincialis along the China coast: clues from mitochondrial cytochrome c oxidase I sequences

As an introduced species, Mytilus galloprovincialis has developed into self-sustaining naturalized populations and has been widely cultivated in northern China. The M. galloprovincialis aquaculture industry wholly depends on the movement of naturalized juveniles onto farms. It is, therefore, necessary to understand the genetic effect of continuous spats’ translocation. This study divided 12 localities of M. galloprovincialis along the China coast into three types of populations—farmed, naturalized adjacent farmed, and isolated—to investigate the genetic variation and differentiation. The genetic variability is reflected by haplotype diversity, nucleotide diversity, and the mean number of pairwise differences expressed as farmed populations > naturalized adjacent farmed populations > isolated populations. The Hierarchical analyses and Mantel-test indicated slight divergence between farmed and naturalized populations, northern and southern populations. The farmed and naturalized populations clustered into two separate categories in the neighbor-joining tree except two anthropogenically intervened localities. The present results suggest that the translocation practice positively affected genetic variability and played a vital role in shaping genetic composition. The information obtained in this study provides new insights into the impacts of the translocation culture model of marine mollusks

Advancing Control for Shield Tunneling Machine by Backstepping Design with LuGre Friction Model

Shield tunneling machine is widely applied for underground tunnel construction. The shield machine is a complex machine with large momentum and ultralow advancing speed. The working condition underground is rather complicated and unpredictable, and brings big trouble in controlling the advancing speed. This paper focused on the advancing motion control on desired tunnel axis. A three-state dynamic model was established with considering unknown front face earth pressure force and unknown friction force. LuGre friction model was introduced to describe the friction force. Backstepping design was then proposed to make tracking error converge to zero. To have a comparison study, controller without LuGre model was designed. Tracking simulations of speed regulations and simulations when front face earth pressure changed were carried out to show the transient performances of the proposed controller. The results indicated that the controller had good tracking performance even under changing geological conditions. Experiments of speed regulations were carried out to have validations of the controllers

Expression of ICOSL is associated with decreased survival in invasive breast cancer

Background Inducible co-stimulator (ICOS) is a CD28-related molecule exclusively expressed on activated T cells and plays a critical role in modulating the immune response in breast cancer. The blockage of ICOS pathway has been shown to inhibit the activity of Type 2 T helper cells, thus potentially protecting against cancer growth. The current study aims to investigate the correlation between inducible co-stimulator ligand (ICOSL) expression in tumor tissues and the prognoses of patients with invasive breast cancer. Methods Tumor samples from 562 Chinese patients with invasive breast carcinomas were collected between 2003 and 2010. The expression of ICOSL on breast tumor and adjacent non-cancerous tissue was determined via immunohistochemistry. The overall survival (OS) of patients with positive and negative ICOSL expression were described using Kaplan–Meier curves, respectively. Parametric correlation method was used to analyze the correlation between ICOSL expression and other clinicopathological parameters. ICOSL was selected as a dependent variable for multivariate analysis. Results Positive ICOSL expression was identified on the plasma membrane in both cytoplasm and the nucleus of breast cancer cells. Membrane-expressed ICOSL is determined as an independent prognostic factor for OS in breast cancer but without significantly correlating with other clinicopathologic parameters such as age, menopausal status, depth of invasion, lymph node metastasis status, histologic classification, etc. Conclusion Our study suggests that the up-regulated expression of ICOSL protein in breast tumor cells can be associated with poor prognoses in invasive breast carcinomas

Observed wintertime tidal and subtidal currents over the continental shelf in the northern South China Sea

Author Posting. © American Geophysical Union, 2014. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 119 (2014): 5289–5310, doi:10.1002/2014JC009931.Synthesis analyses were performed to examine characteristics of tidal and subtidal currents at eight mooring sites deployed over the northern South China Sea (NSCS) continental shelf in the 2006–2007 and 2009–2010 winters. Rotary spectra and harmonic analysis results showed that observed tidal currents in the NSCS were dominated by baroclinic diurnal tides with phases varying both vertically and horizontally. This feature was supported by the CC-FVCOM results, which demonstrated that the diurnal tidal flow over this shelf was characterized by baroclinic Kelvin waves with vertical phase differences varying in different flow zones. The northeasterly wind-induced southwestward flow prevailed over the NSCS shelf during winter, with episodic appearances of mesoscale eddies and a bottom-intensified buoyancy-driven slope water intrusion. The moored current records captured a warm-core anticyclonic eddy, which originated from the southwestern coast of Taiwan and propagated southwestward along the slope consistent with a combination of β-plane and topographic Rossby waves. The eddy was surface-intensified with a swirl speed of >50 cm/s and a vertical scale of ∼400 m. In absence of eddies and onshore deep slope water intrusion, the observed southwestward flow was highly coherent with the northeasterly wind stress. Observations did not support the existence of the permanent wintertime South China Sea Warm Current (SCSWC). The definition of SCSWC, which was based mainly on thermal wind calculations with assumed level of no motion at the bottom, needs to be interpreted with caution since the observed circulation over the NSCS shelf in winter included both barotropic and baroclinic components.R. Li was supported by the SOA 908 Special Project Foundation of China (908-01-ST07 and 908-01-BC10), the National High Tech Project Foundation (863) of China (2008AA09A401), the Administrator Foundation of South Branch, SOA (0683). The development of FVCOM was funded by the US NSF Office of Polar Programs through grants ARC0712903, ARC0732084, ARC0804029, and ARC1203393.2015-02-1

Monoclonal Antibodies against Accumulation-Associated Protein Affect EPS Biosynthesis and Enhance Bacterial Accumulation of Staphylococcus epidermidis

Because there is no effective antibiotic to eradicate Staphylococcus epidermidis biofilm infections that lead to the failure of medical device implantations, the development of anti-biofilm vaccines is necessary. Biofilm formation by S. epidermidis requires accumulation-associated protein (Aap) that contains sequence repeats known as G5 domains, which are responsible for the Zn2+-dependent dimerization of Aap to mediate intercellular adhesion. Antibodies against Aap have been reported to inhibit biofilm accumulation. In the present study, three monoclonal antibodies (MAbs) against the Aap C-terminal single B-repeat construct followed by the 79-aa half repeat (AapBrpt1.5) were generated. MAb18B6 inhibited biofilm formation by S. epidermidis RP62A to 60% of the maximum, while MAb25C11 and MAb20B9 enhanced biofilm accumulation. All three MAbs aggregated the planktonic bacteria to form visible cell clusters. Epitope mapping revealed that the epitope of MAb18B6, which recognizes an identical area within AapBrpt constructs from S. epidermidis RP62A, was not shared by MAb25C11 and MAb20B9. Furthermore, all three MAbs were found to affect both Aap expression and extracellular polymeric substance (EPS, including extracellular DNA and PIA) biosynthesis in S. epidermidis and enhance the cell accumulation. These findings contribute to a better understanding of staphylococcal biofilm formation and will help to develop epitope-peptide vaccines against staphylococcal infections

The Genomes of Oryza sativa: A History of Duplications

We report improved whole-genome shotgun sequences for the genomes of indica and japonica rice, both with multimegabase contiguity, or almost 1,000-fold improvement over the drafts of 2002. Tested against a nonredundant collection of 19,079 full-length cDNAs, 97.7% of the genes are aligned, without fragmentation, to the mapped super-scaffolds of one or the other genome. We introduce a gene identification procedure for plants that does not rely on similarity to known genes to remove erroneous predictions resulting from transposable elements. Using the available EST data to adjust for residual errors in the predictions, the estimated gene count is at least 38,000–40,000. Only 2%–3% of the genes are unique to any one subspecies, comparable to the amount of sequence that might still be missing. Despite this lack of variation in gene content, there is enormous variation in the intergenic regions. At least a quarter of the two sequences could not be aligned, and where they could be aligned, single nucleotide polymorphism (SNP) rates varied from as little as 3.0 SNP/kb in the coding regions to 27.6 SNP/kb in the transposable elements. A more inclusive new approach for analyzing duplication history is introduced here. It reveals an ancient whole-genome duplication, a recent segmental duplication on Chromosomes 11 and 12, and massive ongoing individual gene duplications. We find 18 distinct pairs of duplicated segments that cover 65.7% of the genome; 17 of these pairs date back to a common time before the divergence of the grasses. More important, ongoing individual gene duplications provide a never-ending source of raw material for gene genesis and are major contributors to the differences between members of the grass family