958 research outputs found
Hawking radiation, W-infinity algebra and trace anomalies
We apply the "trace anomaly method" to the calculation of moments of the
Hawking radiation of a Schwarzschild black hole. We show that they can be
explained as the fluxes of chiral currents forming a W-infinity algebra. Then
we construct the covariant version of these currents and verify that up to
order 6 they are not affected by any trace anomaly. Using cohomological methods
we show that actually, for the fourth order current, no trace anomalies can
exist. The results reported here are strictly valid in two dimensions.Comment: 22 pages, typos correcte
Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre.
Photosynthesis uses a limited range of the solar spectrum, so enhancing spectral coverage could improve the efficiency of light capture. Here, we show that a hybrid reaction centre (RC)/yellow fluorescent protein (YFP) complex accelerates photosynthetic growth in the bacterium Rhodobacter sphaeroides. The structure of the RC/YFP-light-harvesting 1 (LH1) complex shows the position of YFP attachment to the RC-H subunit, on the cytoplasmic side of the RC complex. Fluorescence lifetime microscopy of whole cells and ultrafast transient absorption spectroscopy of purified RC/YFP complexes show that the YFP-RC intermolecular distance and spectral overlap between the emission of YFP and the visible-region (QX) absorption bands of the RC allow energy transfer via a Förster mechanism, with an efficiency of 40±10%. This proof-of-principle study demonstrates the feasibility of increasing spectral coverage for harvesting light using non-native genetically-encoded light-absorbers, thereby augmenting energy transfer and trapping in photosynthesis
Diffusion in supersonic, turbulent, compressible flows
We investigate diffusion in supersonic, turbulent, compressible flows.
Supersonic turbulence can be characterized as network of interacting shocks. We
consider flows with different rms Mach numbers and where energy necessary to
maintain dynamical equilibrium is inserted at different spatial scales. We find
that turbulent transport exhibits super-diffusive behavior due to induced bulk
motions. In a comoving reference frame, however, diffusion behaves normal and
can be described by mixing length theory extended into the supersonic regime.Comment: 11 pages, incl. 5 figures, accepted for publication in Physical
Review E (a high-resolution version is available at
http://www.aip.de./~ralf/Publications/p21.abstract.html
Observation of Parity Violation in the Omega-minus -> Lambda + K-minus Decay
The alpha decay parameter in the process Omega-minus -> Lambda + K-minus has
been measured from a sample of 4.50 million unpolarized Omega-minus decays
recorded by the HyperCP (E871) experiment at Fermilab and found to be [1.78 +/-
0.19(stat) +/- 0.16(syst)]{\times}10^{-2}. This is the first unambiguous
evidence for a nonzero alpha decay parameter, and hence parity violation, in
the Omega-minus -> Lambda + K-minus decay.Comment: 10 pages, 7 figure
Identification of placental nutrient transporters associated with intrauterine growth restriction and pre-eclampsia.
Gestational disorders such as intrauterine growth restriction (IUGR) and pre-eclampsia (PE) are main causes of poor perinatal outcomes worldwide. Both diseases are related with impaired materno-fetal nutrient transfer, but the crucial transport mechanisms underlying IUGR and PE are not fully elucidated. In this study, we aimed to identify membrane transporters highly associated with transplacental nutrient deficiencies in IUGR/PE.
In silico analyses on the identification of differentially expressed nutrient transporters were conducted using seven eligible microarray datasets (from Gene Expression Omnibus), encompassing control and IUGR/PE placental samples. Thereby 46 out of 434 genes were identified as potentially interesting targets. They are involved in the fetal provision with amino acids, carbohydrates, lipids, vitamins and microelements. Targets of interest were clustered into a substrate-specific interaction network by using Search Tool for the Retrieval of Interacting Genes. The subsequent wet-lab validation was performed using quantitative RT-PCR on placentas from clinically well-characterized IUGR/PE patients (IUGR, nâ=â8; PE, nâ=â5; PE+IUGR, nâ=â10) and controls (term, nâ=â13; preterm, nâ=â7), followed by 2D-hierarchical heatmap generation. Statistical evaluation using Kruskal-Wallis tests was then applied to detect significantly different expression patterns, while scatter plot analysis indicated which transporters were predominantly influenced by IUGR or PE, or equally affected by both diseases. Identified by both methods, three overlapping targets, SLC7A7, SLC38A5 (amino acid transporters), and ABCA1 (cholesterol transporter), were further investigated at the protein level by western blotting. Protein analyses in total placental tissue lysates and membrane fractions isolated from disease and control placentas indicated an altered functional activity of those three nutrient transporters in IUGR/PE.
Combining bioinformatic analysis, molecular biological experiments and mathematical diagramming, this study has demonstrated systematic alterations of nutrient transporter expressions in IUGR/PE. Among 46 initially targeted transporters, three significantly regulated genes were further investigated based on the severity and the disease specificity for IUGR and PE. Confirmed by mRNA and protein expression, the amino acid transporters SLC7A7 and SLC38A5 showed marked differences between controls and IUGR/PE and were regulated by both diseases. In contrast, ABCA1 may play an exclusive role in the development of PE
Expression, purification and characterization of the soluble Cu-A domain of cytochrome c oxidase of Paracoccus versutus
Macromolecular Biochemistr
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