An Improved Approach for Mapping Quantitative Trait Loci in a Pseudo-Testcross: Revisiting a Poplar Mapping Study

A pseudo-testcross pedigree is widely used for mapping quantitative trait loci (QTL) in outcrossing species, but the model for analyzing pseudo-testcross data borrowed from the inbred backcross design can only detect those QTLs that are heterozygous only in one parent. In this study, an intercross model that incorporates the high heterozygosity and phase uncertainty of outcrossing species was used to reanalyze a published data set on QTL mapping in poplar trees. Several intercross QTLs that are heterozygous in both parents were detected, which are responsible not only for biomass traits, but also for their genetic correlations. This study provides a more complete identification of QTLs responsible for economically important biomass traits in poplars

Stem Cell Tracking Technologies for Neurological Regenerative Medicine Purposes

The growing field of stem cell therapy is moving toward clinical trials in a variety of applications, particularly for neurological diseases. However, this translation of cell therapies into humans has prompted a need to create innovative and breakthrough methods for stem cell tracing, to explore the migration routes and its reciprocity with microenvironment targets in the body, to monitor and track the outcome after stem cell transplantation therapy, and to track the distribution and cell viability of transplanted cells noninvasively and longitudinally. Recently, a larger number of cell tracking methods in vivo were developed and applied in animals and humans, including magnetic resonance imaging, nuclear medicine imaging, and optical imaging. This review has been intended to summarize the current use of those imaging tools in tracking stem cells, detailing their main features and drawbacks, including image resolution, tissue penetrating depth, and biosafety aspects. Finally, we address that multimodality imaging method will be a more potential tracking tool in the future clinical application

A BAC-Based Physical Map of Zhikong Scallop (Chlamys farreri Jones et Preston)

Zhikong scallop (Chlamys farreri) is one of the most economically important aquaculture species in China. Physical maps are crucial tools for genome sequencing, gene mapping and cloning, genetic improvement and selective breeding. In this study, we have developed a genome-wide, BAC-based physical map for the species. A total of 81,408 clones from two BAC libraries of the scallop were fingerprinted using an ABI 3130xl Genetic Analyzer and a fingerprinting kit developed in our laboratory. After data processing, 63,641 (∼5.8× genome coverage) fingerprints were validated and used in the physical map assembly. A total of 3,696 contigs were assembled for the physical map. Each contig contained an average of 10.0 clones, with an average physical size of 490 kb. The combined total physical size of all contigs was 1.81 Gb, equivalent to approximately 1.5 fold of the scallop haploid genome. A total of 10,587 BAC end sequences (BESs) and 167 markers were integrated into the physical map. We evaluated the physical map by overgo hybridization, BAC-FISH (fluorescence in situ hybridization), contig BAC pool screening and source BAC library screening. The results have provided evidence of the high reliability of the contig physical map. This is the first physical map in mollusc; therefore, it provides an important platform for advanced research of genomics and genetics, and mapping of genes and QTL of economical importance, thus facilitating the genetic improvement and selective breeding of the scallop and other marine molluscs

Controlled Synthesis of Carbon Nanoparticles in a Supercritical Carbon Disulfide System

Carbon nanoparticles with large surface areas were produced by the reduction of carbon disulfide with metallic lithium at 500 °C. The carbon nanoparticles account for about 80% of the carbon product. The carbon nanoparticles were characterized by X-ray powder diffraction, field emission scanning electron microscopy, transmission electron microscopy, high resolution transmission electron microscopy and N2 physisorption. The results showed that carbon nanoparticles predominate in the product. The influence of experimental conditions was investigated, which indicated that temperature plays a crucial role in the formation of carbon nanoparticles. The possible formation mechanism of the carbon nanoparticles was discussed. This method provides a simple and efficient route to the synthesis of carbon nanoparticles

Effect of water head on the permeability of foam-conditioned sands: Experimental and analytical investigation

The water permeability of conditioned soils is one of the most essential properties for Earth Pressure Balance (EPB) tunnelling in coarse-grained soils. Permeability tests are conducted to study the influence of water heads on the permeability of foam-conditioned sands. The initial permeability coefficient of foam-conditioned sands increases with the water head, while the stable permeability coefficient and the initial stable period duration decrease. Meanwhile, a novel analytical model is proposed to estimate the initial permeability coefficient. In this model, the effect of the water head on the initial permeability coefficient is incorporated by calculating void ratios of the foam and effective diameters of foam bubbles under different water pressures. Experimental results are in close agreement with analytical solutions, indicating the excellent performance of the proposed calculation method. In addition, the physical mechanisms of how the water head affects the permeability of foam-conditioned sands are discussed from the contraction and evolution of foam bubbles

Preparation and Magnetic Property of Carbon Nanotube/NiZnCo Ferrite Composite

Precipitation hydrothermal method was used to synthesis carbon nanotubes (CNTs) /Ni0.5Zn0.45Co0.05Fe2O4 composites, and then lwt%CNTs/Ni0.5Zn0.45Co0.05Fe2O4 ceramics were fabricated by spark plasma sintering (SPS) technique. The phase, microstructure and magnetic properties of 1wt%CNTs/Ni0.5Zn0.45Co0.05Fe2O4 were investigated. The results show that, the substitution of zinc ions by cobalt didn't change the spinel phase of Ni-Zn ferrite. In the SPS process, CNTs promoted the sintering kinetics, and the liquid state of Ni can act as sintering aid to accelerate the grain boundary extension. After sintered by SPS at 750 degrees C for 5min, the density of the bulk is about 5.2g/cm(3), the grain size increased to 91m, and the saturation magnetization of the lwt%CNTs/Ni0.5Zn0.45Co0.05Fe2O4 composite ceramics reached at 79.7emu/g