Estimating heritability using family and unrelated individuals data

For the family data from Genetic Analysis Workshop 17, we obtained heritability estimates of quantitative traits Q1 and Q4 using the ASSOC program in the S.A.G.E. software package. ASSOC is a family-based method that estimates heritability through the estimation of variance components. The covariate-adjusted mean heritability was 0.650 for Q1 and 0.745 for Q4. For the unrelated individuals data, we estimated the heritability of Q1 as the proportion of total variance that can be accounted for by all single-nucleotide polymorphisms under an additive model. We examined a novel ordinary least-squares method, a naïve restricted maximum-likelihood method, and a calibrated restricted maximum-likelihood method. We applied the different methods to all 200 replicates for Q1. We observed that the ordinary least-squares method yielded many estimates outside the interval [0, 1]. The restricted maximum-likelihood estimates were more stable than the ordinary least-squares estimates. The naïve restricted maximum-likelihood method yielded an average estimate of 0.462 ± 0.1, and the calibrated restricted maximum-likelihood method yielded an average of 0.535 ± 0.121. Our results demonstrate discrepancies in heritability estimates using the family data and the unrelated individuals data

An Experimental Investigation of the Impact of Surface Tension and Viscosity on the Atomization Effect of a Solid Cone Nozzle

To investigate the influence of surface tension and viscosity on the atomization performance of solid cone nozzles and improve their dust reduction efficiency in industrial and mining enterprises, this study employed a self-built PDPA dust-fog coupling experimental system to explore the effects of different surface tension and viscosity solutions on atomization performance from three aspects: axial, radial, and fog field distribution. The experimental results indicate that compared with surface tension, surface tension has a greater influence on droplet size and velocity in the axial direction. In the radial direction, increasing surface tension and reducing viscosity within a certain range can make the droplet size and velocity distribution more uniform. Additionally, surface tension and viscosity significantly affect the fog field distribution. It was found that a decrease in surface tension can result in a closer proximity of the droplet velocity and size expansion area to the nozzle, while an increase in viscosity can lead to a more prolonged stable area. Furthermore, optimizing the surface tension and viscosity can significantly enhance the efficacy of dust reduction for respirable dust. Consequently, the application of the aforementioned atomization principles to regulate the fog field characteristics of solid cone nozzles can effectively mitigate dust in the production process and augment the dust reduction rate of industrial and mining enterprises

Comprehensive Chemical Dust Suppressant Performance Evaluation and Optimization Method

Chemical dust suppression is an effective dust control technology. A dust suppressant component evaluation method that facilitates a complete selection of safe, efficient, and economical chemical materials has not been explored. Considering dust suppression performance, environmental safety, and cost-effectiveness of chemical dust suppressant technology, this study constructs a comprehensive evaluation index system of chemical dust suppressant performance, including the wetting performance, hygroscopic performance, bonding performance, annual cost per unit area, pH value of dust suppression solution, chemical toxicity, and chemical corrosion. Among them, the index characterizing the wetting performance of the solution is the sedimentation wetting time, which is determined by the dust sedimentation experiment; the index characterizing the hygroscopic performance of the solution is the evaporation stability time, which is determined by the evaporation experiment of the solution on the dust surface; the index to characterize the bonding performance of the solution is the surface wind erosion rate, which is determined by the wind erosion experiment of the solution on the dust surface; the toxicity of the solution is evaluated by the LD50 of the solution; the index to characterize the corrosion performance of the solution is the Q235 monthly steel corrosion rate, which is determined by the Q235 steel corrosion test. Corresponding evaluation parameters are determined including sedimentation wetting time, evaporation stabilization time, surface wind erosion rate; annual average use cost per unit area; solution pH value, chemical acute toxicity classification, monthly corrosion rate of Q235 steel, and corresponding standard test methods are also provided. In order to evaluate the comparability of the results, according to the specific requirements of the evaluation index system and the distribution characteristics of the measurement data, the data of each evaluation and detection index are standardized by linear transformation, range transformation and other methods, so that the obtained results are comparable. Considering the differences in the actual performance requirements of dust suppressants in different usage scenarios, the weights of evaluation indicators at all levels can be set independently and flexible. The experimental test data obtained through the example shows that: among the four chemicals selected to participate in the experiment, the comprehensive dust suppression performance score of Triton X-100 solution is in the poor-grade category. The comprehensive dust suppression performances of calcium chloride solution, water, and polyacrylamide solution scored high in the average-grade category. The comprehensive evaluation process is logically correct, and the results are consistent with the phenomena observed in the experiment, consistent with conventional understanding, and have strong credibility. This method can provide a standardized evaluation technique and test process for the comprehensive performance evaluation and comparison of chemical materials and dust suppressants

The Proteolysis of ECM in Intervertebral Disc Degeneration

Intervertebral disc (IVD) degeneration (IDD) is a pathological process that commonly occurs throughout the human life span and is a major cause of lower back pain. Better elucidation of the molecular mechanisms involved in disc degeneration could provide a theoretical basis for the development of lumbar disc intervention strategies. In recent years, extracellular matrix (ECM) homeostasis has received much attention due to its relevance to the mechanical properties of IVDs. ECM proteolysis mediated by a variety of proteases is involved in the pathological process of disc degeneration. Here, we discuss in detail the relationship between the IVD as well as the ECM and the role of ECM proteolysis in the degenerative process of the IVD. Targeting ECM proteolysis-associated proteases may be an effective means of intervention in IDD

Monitoring Earthquake-Damaged Vegetation after the 2008 Wenchuan Earthquake in the Mountainous River Basins, Dujiangyan County

The 2008 Wenchuan earthquake destroyed large areas of vegetation in the Baisha River and Longxi River basins, in Dujiangyan County, China. There were several debris flow events in these mountainous river basins after 2008. Currently, these damaged vegetation areas are in various stages of recovery. This recovery vegetation improves the resistance of slopes to both surficial erosion and mass wasting. We introduce a probabilistic approach to determining the relationships between damaged vegetation and slope materials’ stability, and model the sediment and flow (hydrological) connectivity index to detect the hydrological changes in a given river basin, using the multi-temporal (1994–2014) remote-sensing images to monitor the vegetation recovery processes. Our results demonstrated that the earthquake-damaged vegetation areas have coupling relationships with topographic environment and slope material properties, and can be used to assess the slope material stability. Further, our analysis results showed that the areas with horizontal distance to river streams <500 m are areas that actively contribute sediment to the stream channel network, and are main material sources for debris flow processes in one given mountainous basin

A New Method of Conductor Galloping Monitoring Using the Target Detection of Infrared Source

Because the galloping of iced conductors is one of the main disasters in the State Grid, resulting in huge economic and property losses every year, the research on relevant monitoring methods is of great significance. The existing galloping monitoring technology is mainly based on the contact detection method, which presents potential electrical hazards and power supply problems. In this paper, a conductor galloping monitoring method based on the target detection of infrared sources is put forward to overcome the shortcomings of existing methods. In other words, an infrared source label is installed on the conductor spacer, high-definition night vision infrared cameras are installed on electric power towers to take video of the infrared source labels, and the characteristic amplitude of conductor galloping is calculated by an image recognition and tracking algorithm. The practical application results indicate that the method has the advantages of non-contact detection, safety and reliability, and high detection accuracy

RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway

Accumulating evidence indicates that ER-phagy serves as a key adaptive regulatory mechanism in response to various stress conditions. However, the exact mechanisms underlying ER-phagy in the pathogenesis of intervertebral disc degeneration remain largely unclear. In the present study, we demonstrated that RETREG1-mediated ER-phagy is induced by glucose deprivation (GD) treatment, along with ER stress activation and cell function decline. Importantly, ER-phagy was shown to be crucial for cell survival under GD conditions. Furthermore, ER stress was suggested as an upstream event of ER-phagy upon GD treatment and upregulation of ER-phagy could counteract the ER stress response. Therefore, our findings indicate that RETREG1-mediated ER-phagy activation protects against GD treatment-induced cell injury via modulating ER stress in human nucleus pulposus cells