Cactus pear: a natural product in cancer chemoprevention

BACKGROUND: Cancer chemoprevention is a new approach in cancer prevention, in which chemical agents are used to prevent cancer in normal and/or high-risk populations. Although chemoprevention has shown promise in some epithelial cancers, currently available preventive agents are limited and the agents are costly, generally with side effects. Natural products, such as grape seed, green tea, and certain herbs have demonstrated anti-cancer effects. To find a natural product that can be used in chemoprevention of cancer, we tested Arizona cactus fruit solution, the aqueous extracts of cactus pear, for its anti-cancer effects in cultured cells and in an animal model. METHOD: Aqueous extracts of cactus pear were used to treat immortalized ovarian and cervical epithelial cells, as well as ovarian, cervical, and bladder cancer cells. Aqueous extracts of cactus pear were used at six concentrations (0, 0.5, 1, 5, 10 or 25%) to treat cells for 1, 3, or 5 days. Growth inhibition, apoptosis induction, and cell cycle changes were analyzed in the cultured cells; the suppression of tumor growth in nude mice was evaluated and compared with the effect of a synthetic retinoid N-(4-hydroxyphernyl) retinamide (4-HPR), which is currently used as a chemoprevention agent. Immunohistochemistry staining of tissue samples from animal tumors was performed to examine the gene expression. RESULTS: Cells exposed to cactus pear extracts had a significant increase in apoptosis and growth inhibition in both immortalized epithelial cells and cancer cells in a dose- and time-dependent manner. It also affected cell cycle of cancer cells by increasing G1 and decreasing G2 and S phases. Both 4-HPR and cactus pear extracts significantly suppressed tumor growth in nude mice, increased annexin IV expression, and decreased VEGF expression. CONCLUSION: Arizona cactus pear extracts effectively inhibited cell growth in several different immortalized and cancer cell cultures, suppressed tumor growth in nude mice, and modulated expression of tumor-related genes. These effects were comparable with those caused by a synthetic retinoid currently used in chemoprevention trials. The mechanism of the anti-cancer effects of cactus pear extracts needs to be further studied

Excitability and Threshold Mechanism for Enhanced Neuronal Response Induced by Inhibition Preceding Excitation

Postinhibitory facilitation (PIF) of neural firing presents a paradoxical phenomenon that the inhibitory effect induces enhancement instead of reduction of the firing activity, which plays important roles in sound location of the auditory nervous system, awaited theoretical explanations. In the present paper, excitability and threshold mechanism for the PIF phenomenon is presented in the Morris-Lecar model with type I, II, and III excitabilities. Firstly, compared with the purely excitatory stimulations applied to the steady state, the inhibitory preceding excitatory stimulation to form pairs induces the firing rate increased for type II and III excitabilities instead of type I excitability, when the interval between the inhibitory and excitatory stimulation within each pair is suitable. Secondly, the threshold mechanism for the PIF phenomenon is acquired. For type II and III excitabilities, the inhibitory stimulation induces subthreshold oscillations around the steady state. During the middle and ending phase of the ascending part and the beginning phase of the descending part within a period of the subthreshold oscillations, the threshold to evoke an action potential by an excitatory stimulation becomes weaker, which is the cause for the PIF phenomenon. Last, a theoretical estimation for the range of the interval between the inhibitory and excitatory stimulation for the PIF phenomenon is acquired, which approximates half of the intrinsic period of the subthreshold oscillations for the relatively strong stimulations and becomes narrower for the relatively weak stimulations. The interval for the PIF phenomenon is much shorter for type III excitability, which is closer to the experiment observation, due to the shorter period of the subthreshold oscillations. The results present the excitability and threshold mechanism for the PIF phenomenon, which provide comprehensive and deep explanations to the PIF phenomenon

A Real-Time Pricing Scheme for Energy Management in Integrated Energy Systems: A Stackelberg Game Approach

This paper proposes a real-time pricing scheme for the demand response management between one energy provider and multiple energy hub operators. A promising energy trading scenario has been designed for the near future integrated energy system. The Stackelberg game approach was employed to capture the interactions between the energy provider (leader) and energy consumers (follower). A distributed algorithm was proposed to derive the Stackelberg equilibrium, then, the best strategies for the energy provider and each energy hub operator were explored in order to maximize their benefits. Simulation results showed that the proposed method can balance the energy supply and demand, improve the payoffs for all players, as well as smooth the aggregated load profiles of all energy consumers

A Novel Energy Function-Based Stability Evaluation and Nonlinear Control Approach for Energy Internet

Unlike conventional interconnected power systems, energy Internet presents an unsolved and more challenging problem for the society including transfer impedance, damping, large penetration of distributed generation, and numerous hybrid integration of generators and converters. In this paper, a novel energy function designed for energy internet router is proposed to accurately evaluate its transfer stability. The reliability of the proposed energy function is confirmed through both theoretical analysis and empirical simulations. Furthermore, generalized methods to determine critical stable energy, stable domain, and critical clearing time are proposed. By updating stability criterion and evaluating system energy of post-disturbance system, fault energy-based impulsive feedback control method is specifically designed for energy Internet to stabilize the system. Simulation and experimental results are provided to validate the effectiveness of the proposed energy function and nonlinear control method

Sodiophilic V2O3-Inducing Layer for Long Lifespan and Dendrite-Free Sodium Metal Anodes

Because of the superiority of low cost and high theoretical capacity, sodium metal batteries are considered an attractive option for high energy storage. However, the uncontrollable and random deposition of Na tends to expedite the formation of Na dendrites and increases the risk of thermal runaway. The method of preplant sodiophilic sites can induce the lateral deposition of Na instead of sharp dendrite emergence. Here, we introduce the sodiophilic V2O3 particles to form a protective layer on Na surface (Na/V2O3). The high Na ion adsorption energy and low nucleation overpotential of Na/V2O3 facilitate the diffusion of Na ions and homogeneous Na deposition, which can work well in cubing dendrite development. Thus, the symmetrical cell (Na/V2O3||Na/V2O3) can stably operate for 670 h at 0.5 mA·cm−2/1 mAh·cm−2 with a smaller voltage hysteresis (less than 100 mV). Moreover, full cell constructed by coupling Na/V2O3 anode with Na3V2(PO4)3 cathode displays an outstanding rate performance, maintaining a high capacity of 70 mAh·g−1 at 30 C. On the basis of the design of sodiophilic protection layer, a dendrite-free, outstanding rate performance, and long lifespan sodium metal battery is realized