Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia

A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia

Les analysis on cylinder cascade flow based on energy ratio coefficient

The flow field around the cylinder cascade is widely used to analyze the interaction of vortex shedding and the information on heat transfer. Large eddy simulation (LES) can be used to get the turbulent flow information in detail. The resolved largescale structures are determined by the size of the grid, and the turbulent vortex dissipation is modeled with a subgrid scale model. Whereas there is no accurate criterion to provide the subgrid scale with the physical meaning. Based on turbulent energy ratio coefficient and numerical simulation results with turbulent model, the subgrid was generated for the incompressible fluid flowing around a column of cylinder cascade with a gap-to-diameter ratio of 2. Smagorinsky-Lily (SM) model was applied to LES analysis. The turbulent flow information was compared with the experimental data by PIV. Two cases with different Reynolds numbers were studied. When the turbulent energy ratio coefficient reached to 30%- 40%, the turbulent dissipation could be captured by LES method with less grid number. The large scale vortex interaction behind the cylinder cascade was analyzed further. It is verified that LES method can be used for engineering based on the turbulent energy ratio coefficient with acceptable computational cost.papers presented to the 12th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Costa de Sol, Spain on 11-13 July 2016

Measurement of Trace I-129 Concentrations in CsI Powder and Organic Liquid Scintillator with Accelerator Mass Spectrometry

Levels of trace radiopurity in active detector materials is a subject of major concern in low-background experiments. Procedures were devised to measure trace concentrations of I-129 in the inorganic salt CsI as well as in organic liquid scintillator with Accelerator Mass Spectrometry (AMS) which leads to improvement in sensitivities by several orders of magnitude over other methods. No evidence of their existence in these materials were observed. Limits of < 6 X 10^{-13} g/g and < 2.6 X 10^{-17} g/g on the contaminations of I-129 in CsI and liquid scintillator, respectively, were derived.These are the first results in a research program whose goals are to develop techniques to measure trace radioactivity in detector materials by AMS.Comment: Proceedings of 10th International Conference on Accelerator Mass Spectrometr

Collective Modes of Soliton-Lattice States in Double-Quantum-Well Systems

In strong perpendicular magnetic fields double-quantum-well systems can sometimes occur in unusual broken symmetry states which have interwell phase coherence in the absence of interwell hopping. When hopping is present in such systems and the magnetic field is tilted away from the normal to the quantum well planes, a related soliton-lattice state can occur which has kinks in the dependence of the relative phase between electrons in opposite layers on the coordinate perpendicular to the in-plane component of the magnetic field. In this article we evaluate the collective modes of this soliton-lattice state in the generalized random-phase aproximation. We find that, in addition to the Goldstone modes associated with the broken translational symmetry of the soliton-lattice state, higher energy collective modes occur which are closely related to the Goldstone modes present in the spontaneously phase-coherent state. We study the evolution of these collective modes as a function of the strength of the in-plane magnetic field and comment on the possibility of using the in-plane field to generate a finite wave probe of the spontaneously phase-coherent state.Comment: REVTEX, 37 pages (text) and 15 uuencoded postscript figure

Correlations, compressibility, and capacitance in double-quantum-well systems in the quantum Hall regime

In the quantum Hall regime, electronic correlations in double-layer two-dimensional electron systems are strong because the kinetic energy is quenched by Landau quantization. In this article we point out that these correlations are reflected in the way the partitioning of charge between the two-layers responds to a bias potential. We report on illustrative calculations based on an unrestricted Hartree-Fock approximation which allows for spontaneous inter-layer phase coherence. The possibility of studying inter-layer correlations by capacitive coupling to separately contacted two-dimensional layers is discussed in detail.Comment: RevTex style, 21 pages, 6 postscript figures in a separate file; Phys. Rev. B (in press

Simulating (electro)hydrodynamic effects in colloidal dispersions: smoothed profile method

Previously, we have proposed a direct simulation scheme for colloidal dispersions in a Newtonian solvent [Phys.Rev.E 71,036707 (2005)]. An improved formulation called the ``Smoothed Profile (SP) method'' is presented here in which simultaneous time-marching is used for the host fluid and colloids. The SP method is a direct numerical simulation of particulate flows and provides a coupling scheme between the continuum fluid dynamics and rigid-body dynamics through utilization of a smoothed profile for the colloidal particles. Moreover, the improved formulation includes an extension to incorporate multi-component fluids, allowing systems such as charged colloids in electrolyte solutions to be studied. The dynamics of the colloidal dispersions are solved with the same computational cost as required for solving non-particulate flows. Numerical results which assess the hydrodynamic interactions of colloidal dispersions are presented to validate the SP method. The SP method is not restricted to particular constitutive models of the host fluids and can hence be applied to colloidal dispersions in complex fluids

Reconstructing interacting new agegraphic polytropic gas model in non-flat FRW universe

We study the correspondence between the interacting new agegraphic dark energy and the polytropic gas model of dark energy in the non-flat FRW universe. This correspondence allows to reconstruct the potential and the dynamics for the scalar field of the polytropic model, which describe accelerated expansion of the universe.Comment: 9 page

Statefinder diagnostic and stability of modified gravity consistent with holographic and new agegraphic dark energy

Recently one of us derived the action of modified gravity consistent with the holographic and new-agegraphic dark energy. In this paper, we investigate the stability of the Lagrangians of the modified gravity as discussed in [M. R. Setare, Int. J. Mod. Phys. D 17 (2008) 2219; M. R. Setare, Astrophys. Space Sci. 326 (2010) 27]. We also calculate the statefinder parameters which classify our dark energy model.Comment: 12 pages, 2 figures, accepted by Gen. Relativ. Gravi