Late cardioprotection of exercise preconditioning against exhaustive exercise-induced myocardial injury by up-regulatation of connexin 43 expression in rat hearts

AbstractObjectiveTo investigate the expression of myocardium connexin 43 (Cx43) in late exercise preconditioning (LEP) cardioprotection.MethodsEight-week-old adult male Sprague Dawley rats were randomly assigned into four groups (n = 8). Myocardial injury was judged in accordance with serum levels of cTnⅠ and NT-proBNP as well as hematoxylin basicfuchsin picric acid staining of myocardium. Cx43 mRNA was detected by in situ hybridization and qualified by real-time fluorescence quantitative PCR. Cx43 protein was localized by immunohistochemistry and its expression level was determined by western blotting.ResultsThe LEP obviously attenuated the myocardial ischemia/hypoxia injury caused by exhaustive exercise. There was no significant difference of Cx43 mRNA level between the four groups. Cx43 protein level was decreased significantly in group EE (P < 0.05). However, LEP produced a significant increase in Cx43 protein level (P < 0.05), and the decreased Cx43 protein level in exhaustive exercise was significantly up-regulated by LEP (P < 0.05).ConclusionsLEP protects rat heart against exhaustive exercise-induced myocardial injury by up-regulating the expression of myocardial Cx43

(Anthracen-9-yl)(piperidin-1-yl)­methanone

The title compound, C20H19NO, is a substructure of CP-640186, a potent inhibitor of mammalian acetyl-coenzyme A carboxyl­ases. In the crystal structure, the amide group forms a dihedral angle of 87.0 (1)° with the plane of the anthracene unit and the piperidine ring adopts a chair conformation. Mol­ecules are arranged into layers parallel to (100) and adjacent anthracene units within layers form dihedral angles of 13.2 (1)°. C—H⋯O inter­actions from the piperidine rings to the C=O group of the amide are observed between layers

Covert Laparoscopic Cholecystectomy: A New Minimally Invasive Technique

To further improve our developed transumbilical endoscopic surgery (TUES), we developed a completely covert laparoscopic cholecystectomy (LC). Twelve cases of LC were recruited for this new approach. First, a 10-mm trocar was placed above the umbilicus for inserting the laparoscope. Two 5-mm trocars were then placed near the right and left ends of the superior margin of the suprapubic hair. After the 5-mm 30° laparoscope was shifted to the left suprapubic trocar, the harmonic scalper, electric hook, and grasper were inserted either through the 10-mm umbilical trocar or through the right suprapubic trocar. All gallbladders were successfully removed without intraoperative complications. The mean operating time was 28.5±5.7min (range 20-45min). All patients felt well after surgery and did not need postoperative analgesia. They resumed free oral intake 6h after the procedure. All patients were satisfied with the appearance of the incisions, which were completely hidden in the umbilicus and suprapubic hair. The approach we developed has overcome both external instrument interference around the umbilicus and the loss of triangulation in the operative field. It is relatively simpler than a typical TUES and offers better cosmetic results

A polymorph of diaqua­bis(pyrazine-2-carboxyl­ato-κ2 N 1,O)copper(II)

The title compound, [Cu(C5H3N2O2)2(H2O)2], is a new polymorph of the previously reported compound [Klein et al. (1982 ▶). Inorg. Chem. 21, 1891–1897]. The CuII atom, lying on an inversion center, is coordinated by two N atoms and two O atoms from two pyrazine-2-carboxyl­ate ligands and by two water mol­ecules in a distorted octa­hedral geometry with the water mol­ecules occupying the axial sites. Inter­molecular O—H⋯O, O—H⋯N and C—H⋯O hydrogen bonds connect the complex mol­ecules into a two-dimensional layer parallel to (10), whereas the previously reported polymorph exhibits a three-dimensional hydrogen-bonded network

catena-Poly[diammonium [diaqua­bis(pyridine-2,4-dicarboxyl­ato-κ2 N,O 2)cuprate(II)] [[diaqua­copper(II)]-μ-pyridine-2,4-dicarboxyl­ato-κ3 N,O 2:O 2′-[tetra­aqua­cadmium(II)]-μ-pyridine-2,4-dicarboxyl­ato-κ3 O 2:N,O 2′] hexa­hydrate]

The title mixed-metal complex, {(NH4)2[Cu(C7H3NO4)2(H2O)2][CdCu(C7H3NO4)2(H2O)6]·6H2O}n, contains one octa­hedrally coordinated CdII center and two octa­hedrally coordinated CuII centers, each lying on an inversion center. The two CuII atoms are each coordinated by two O atoms and two N atoms from two 2,4-pydc (2,4-H2pydc = pyridine-2,4-dicarboxylic acid) ligands in the equatorial plane and two water mol­ecules at the axial sites, thus producing two crystallographically independent [Cu(2,4-pydc)2(H2O)2]2− metalloligands. One metalloligand exists as a discrete anion and the other connects the Cd(H2O)4 units, forming a neutral chain. O—H⋯O and N—H⋯O hydrogen bonds connects the polymeric chains, complex anions, ammonium cations and uncoordinated water mol­ecules into a three-dimensional supra­molecular network