TROP2 promotes proliferation, migration and metastasis of gallbladder cancer cells by regulating PI3K/AKT pathway and inducing EMT

The human trophoblast cell surface antigen 2 (TROP2) is overexpressed in many cancers. However, its effect on proliferation, migration and metastasis of gallbladder cancer remains unclear. In this study, we found that TROP2 was highly expressed in gallbladder cancer. Overexpression of TROP2 was associated with poor prognosis. Knockdown of TROP2 in gallbladder cancer cell lines strongly inhibited the cell proliferation, clone formation, invasion and migration in vitro, while TROP2 overexpression had opposite effects. In addition, knockdown of TROP2 increased the expression of total PTEN, p-PTEN and PDK-1 but reduced p-AKT via PI3K/AKT pathway. TROP2 downregulation also inhibited vimentin and increased E-cadherin expression during epithelial-mesenchymal transition (EMT). Moreover, gallbladder cancer cells with TROP2 knockdown formed smaller xenografted tumors in vivo. In consistent with in vitro results, TROP2 inhibition decreased Akt phosphorylation, increased PTEN expression and postponed EMT of gallbladder cancer cells in vivo. In conclusion, we revealed that TROP2 promoted the proliferation, migration and metastasis of gallbladder cancer cells by regulating PI3K/ AKT pathway and inducing EMT. TROP2 could serve as a potential prognostic biomarker and therapeutic target for the clinical management of gallbladder cancer

Endoscopic Video-assisted Excision Combined with Mammotome for Major Benign Breast Lesions

BackgroundIt was difficult to excise major benign breast lesions more than 3 cm employing either Mammotome or Endoscopic respectively.MethodsFrom October,2005 and October,2008,10 patients with major benign breast lesions underwent endoscopic,video-assisted breast excision combined with Mammotome in our institution.Cosmetic results were evaluated using the ABNSW scoring system.ResultsThe incision size was 1 to 1.8 cm.The operation time ranged from 30 to 55 minutes with a median of 41 minutes of all patients,from 38 to 55 minutes with a median of 45 minutes of 6 patients with rigid mass and from 30 to 36 minutes with a median of 30 minutes of 4 patients with flexible mass.The median total ABNSW score was 14.1 points,median intraoperative bleeding was 17 ml,and median cost was 7825 ￥.No clinically important complication was encountered,and all patients were extremely satisfied with the cosmetic results of the procedure.ConclusionVideo-assisted endoscopic resection combined with Mammotome is a safe,effective technique to treat major benign breast tumors,and provides esthetic advantages

The prognostic value of negative lymph node count for patients with gastric cancer who received preoperative radiotherapy

Negative lymph node (NLN) count provides accurate prognostic information in patients with gastric cancer. However, it is unclear whether NLN still has prognostic value for patients received preoperative radiotherapy. In this study, Surveillance, Epidemiology, and End Results Program (SEER)-registered gastric cancer patients were used for analysis. Clinicopathological characteristics and survival time were collected. Univariate and multivariate Cox proportional hazards models were used to assess the risk factors for survival. NLN count was validated as an independent prognostic factor in both univariate and mulivariate analysis (P < 0.001). X-tile plots identified 12 as the optimal cutoff value to divide the patients into high and low risk subsets in terms of survival rate. Nomogram based on cancer-specific survival was successfully established according to all significant factors. The C-index was 0.630 (95% CI: 0.605–0.655). Subgroup analysis showed that NLN count was a prognosis factor for patients with advanced gastric cancer (stage ypII and ypIII). In conclusion, our results firmly demonstrated that NLN count was an independent prognostic factor for patients with gastric cancer who received preoperative radiotherapy. It provides more accurate prognostic information especially for patients with advanced gastric cancer (stage ypII and ypIII). Nomograms based on cancer-specific survival could be recommended as practical models to evaluate prognosis

Erratum: TROP2 promotes proliferation, migration and metastasis of gallbladder cancer cells by regulating PI3K/AKT pathway and inducing EMT (Oncotarget (2017) 8 (47052–47063) DOI: 10.18632/oncotarget.16789)

This article has been corrected: Due to errors in image processing, Figure 2C contains an image from the wrong cell line (GBC-SD cells). The corrected Figure 2C is shown below. The authors declare that these corrections do not change the results or conclusions of this paper