Tumor-associated macrophages in gliomas—basic insights and treatment opportunities

Glioma refers to a group of primary brain tumors which includes glioblastoma (GBM), astrocytoma and oligodendroglioma as major entities. Among these, GBM is the most frequent and most malignant one. The highly infiltrative nature of gliomas, and their intrinsic intra- and intertumoral heterogeneity, pose challenges towards developing effective treatments. The glioma microenvironment, in addition, is also thought to play a critical role during tumor development and treatment course. Unlike most other solid tumors, the glioma microenvironment is dominated by macrophages and microglia—collectively known as tumor-associated macrophages (TAMs). TAMs, like their homeostatic counterparts, are plastic in nature and can polarize to either pro-inflammatory or immunosuppressive states. Many lines of evidence suggest that immunosuppressive TAMs dominate the glioma microenvironment, which fosters tumor development, contributes to tumor aggressiveness and recurrence and, very importantly, impedes the therapeutic effect of various treatment regimens. However, through the development of new therapeutic strategies, TAMs can potentially be shifted towards a proinflammatory state which is of great therapeutic interest. In this review, we will discuss various aspects of TAMs in the context of glioma. The focus will be on the basic biology of TAMs in the central nervous system (CNS), potential biomarkers, critical evaluation of model systems for studying TAMs and finally, special attention will be given to the potential targeted therapeutic options that involve the TAM compartment in gliomas.publishedVersio

Salt Stress Tolerance in Rice: Emerging Role of Exogenous Phytoprotectants

Excess salinity in soil is one of the major environmental factors that limit plant growth and yield of a wide variety of crops including rice. On the basis of tolerance ability toward salinity, rice is considered as salt-sensitive crop, and growth and yield of rice are greatly affected by salinity. In general, rice can tolerate a small amount of saltwater without compromising the growth and yield. However, it greatly depends on the types and species of rice and their growth stage. Salinity-induced ionic and osmotic stresses reduce rate of photosynthesis and consequently cause oxidative stress, which is also responsible for growth reduction. The negative effects of salt stress that mentioned ultimately reduced yield of most crops including rice, except some halophytes. In recent decades, researchers have developed various approaches toward making salt-tolerant rice varieties. Using phytoprotectants is found to be effective in conferring salt tolerance to rice plants. In this chapter, we reviewed the recent reports on different aspects on salt stress tolerance strategies in light of using phytoprotectants

Una observación hacia los procesos asistidos por computadora en la producción de prendas de vestir. Comparación y análisis de software CAD/CAM en Bangladesh

This research paper inquires about different attributes of Computer-aided processes in garments production. This perspective Research was done by our courageous team from 2021 to 2022. It reveals adequate information on the Garments industry's Intension and criteria for choosing CAD/CAM software. For the sake of this Research, we visited more than 600 industries to gather raw data; Every Industry tried to attend this Research from a different region of Bangladesh willingly. After collecting all raw data from the garments industry. The data of the Garments industry was coordinated by Excel program. Consequently, the data was analyzed and implemented statistically to identify the Garments Industry attribute for satisfaction with CAD/CAM software. This process also detects many challenges and defines and advises a proper solution to the problems that the Garments industry is facing in the current situation. This research paper demonstrates adequate information about the Garment's criteria and demand in purchasing garments Computer-aided software.Este trabajo de investigación indaga sobre los diferentes atributos de los procesos asistidos por computadora en la producción de prendas de vestir. Esta investigación de perspectiva fue realizada por nuestro valiente equipo de 2021 a 2022. Revela información adecuada sobre la intención de la industria de la confección y los criterios para elegir el software CAD/CAM. Por el bien de esta investigación, visitamos más de 600 industrias para recopilar datos sin procesar; Cada industria trató de asistir a esta investigación desde una región diferente de Bangladesh de buena gana. Después de recopilar todos los datos sin procesar de la industria de la confección. Los datos de la industria de Vestuario fueron coordinados por el programa Excel. En consecuencia, los datos se analizaron e implementaron estadísticamente para identificar el atributo de la industria de la confección para la satisfacción con el software CAD/CAM. Este proceso también detecta muchos desafíos y define y aconseja una solución adecuada a los problemas que enfrenta la industria de la confección en la situación actual. Este trabajo de investigación demuestra información adecuada sobre los criterios de la prenda y la demanda en la compra de prendas Software asistido por computadora

Social Safety Net Programs: Contribution to Socio-Economic Resilience of Vulnerable Group

Bangladesh is a disaster affected country due to its geographical location and anthropogenic causes. Every year thousands of people are impacted by different kinds of climate induced environmental stressed. Social Safety Nets (SSNs) are non-contributory programmes that helps the poor and vulnerable community and also to reduce poverty and inequality to cope with disasters. Bangladesh government has taken initiative to alleviate poverty including the most vulnerable segment and poor people under different Safety Net Programmes. This study has been undertaken to assess the impacts of these allowance programs for increasing social and economic resiliency in household level. Both primary and secondary data were used to conduct this study. Primary data were collected through questionnaire survey, focus group discussions, and case study. Secondary data were collected from different sources including books, journals and websites related to the study. This study has also investigated how the allowance allocated to extreme poor widows, elders and disabled people can facilitate their disaster resiliency. It compares the situation of allowance receivers to the situation when they were not getting the allowance. The study found that the program has a positive impact on the basic needs like food, cloth, medicine accessibility of the beneficiaries which make them economically resilient. It also founds that although the allowance amount is relatively small and insufficient to significantly contribute to household’s graduation, it often enables recipients to stabilize their earnings. The study has suggested the need to develop a number of strategies especially for the local government to facilitate successful SSN program including increasing the amount of allowance, distributing money through mobile banking system, proper monitoring in selection process are most urgent in the study area

Oncolytic H-1 Parvovirus Hijacks Galectin-1 to Enter Cancer Cells

Clinical studies in glioblastoma and pancreatic carcinoma patients strongly support the further development of H-1 protoparvovirus (H-1PV)-based anticancer therapies. The identification of cellular factors involved in the H-1PV life cycle may provide the knowledge to improve H-1PV anticancer potential. Recently, we showed that sialylated laminins mediate H-1PV attachment at the cell membrane. In this study, we revealed that H-1PV also interacts at the cell surface with galectin-1 and uses this glycoprotein to enter cancer cells. Indeed, knockdown/out of LGALS1, the gene encoding galectin-1, strongly decreases the ability of H-1PV to infect and kill cancer cells. This ability is rescued by the re-introduction of LGALS1 into cancer cells. Pre-treatment with lactose, which is able to bind to galectins and modulate their cellular functions, decreased H-1PV infectivity in a dose dependent manner. In silico analysis reveals that LGALS1 is overexpressed in various tumours including glioblastoma and pancreatic carcinoma. We show by immunohistochemistry analysis of 122 glioblastoma biopsies that galectin-1 protein levels vary between tumours, with levels in recurrent glioblastoma higher than those in primary tumours or normal tissues. We also find a direct correlation between LGALS1 transcript levels and H-1PV oncolytic activity in 53 cancer cell lines from different tumour origins. Strikingly, the addition of purified galectin-1 sensitises poorly susceptible GBM cell lines to H-1PV killing activity by rescuing cell entry. Together, these findings demonstrate that galectin-1 is a crucial determinant of the H-1PV life cycle.publishedVersio

Resistance and co-resistance of metallo-beta-lactamase genes in diarrheal and urinary tract pathogens in Bangladesh

Carbapenem antibiotics are the drug of choice for treating multidrug-resistant bacterial infections. Metallo-beta-lactamases (MBLs) are carbapenemase capable of hydrolyzing nearly all therapeutically available beta-lactam antibiotics. Consequently, a need to assess the frequency and phenotypic resistance phenomena of two MBL genes in diarrheal and urinary tract infections (UTIs). Samples were collected through a cross-sectional study, with MBLs genes detected via PCR. Two hundred twenty eight diarrheal bacteria were isolated from 240 samples. The most predominant pathogens were Escherichia coli (32%) and Klebsiella spp. (7%). Phenotypic resistance to amoxicillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 50.4%, 65.6%, 66.8%, 80.5%, 54.4%, 41.6%, 25.7%, 41.2%, 37.2%, and 42.9%, respectively. Total 142 UTI pathogens were obtained from 150 urine samples, with Klebsiella spp. (39%) and Escherichia coli (24%) are the major pathogens. Phenotypic resistance to amoxycillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 93.7%, 75.0%, 91.5%, 93.7%, 88.0%, 72.5%, 13.6%, 44.4%, 71.1%, and 43%, respectively. Twenty four diarrheal isolates carried either blaNDM-1 or blaVIM genes; the overall MBL gene prevalence was 10.5%. Thirty six UTI pathogens carried either blaNDM-1 or blaVIM genes (25.4%). Seven isolates carried both blaNDM-1 and blaVIM genes. MBL genes exhibited a strong association with phenotypic carbapenem and other beta-lactam antibiotic resistance. Resistance to carbapenems requires active surveillance and stewardship

Resistance and co-resistance of metallo-beta-lactamase genes in diarrheal and urinary-tract pathogens in Bangladesh

Carbapenems are the antibiotics of choice for treating multidrug-resistant bacterial infections. Metallo-β-lactamases (MBLs) are carbapenemases capable of hydrolyzing nearly all therapeutically available beta-lactam antibiotics. Consequently, this research assessed the distribution of two MBL genes and three β-lactamases and their associated phenotypic resistance in diarrheal and urinary-tract infections (UTIs) to guide future policies. Samples were collected through a cross-sectional study, and β-lactamase genes were detected via PCR. A total of 228 diarrheal bacteria were isolated from 240 samples. The most predominant pathogens were Escherichia coli (32%) and Klebsiella spp. (7%). Phenotypic resistance to amoxicillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 50.4%, 65.6%, 66.8%, 80.5%, 54.4%, 41.6%, 25.7%, 41.2%, 37.2%, and 42.9%, respectively. A total of 142 UTI pathogens were identified from 150 urine samples. Klebsiella spp. (39%) and Escherichia coli (24%) were the major pathogens isolated. Phenotypic resistance to amoxicillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 93.7%, 75.0%, 91.5%, 93.7%, 88.0%, 72.5%, 13.6%, 44.4%, 71.1%, and 43%, respectively. Twenty-four diarrheal isolates carried blaNDM-1 or blaVIM genes. The overall MBL gene prevalence was 10.5%. Thirty-six UTI pathogens carried either blaNDM-1 or blaVIM genes (25.4%). Seven isolates carried both blaNDM-1 and blaVIM genes. MBL genes were strongly associated with phenotypic carbapenem and other β-lactam antibiotic resistance. blaOXA imparted significantly higher phenotypic resistance to β-lactam antibiotics. Active surveillance and stewardship programs are urgently needed to reduce carbapenem resistance in Bangladesh

TGF-β promotes microtube formation in glioblastoma through Thrombospondin 1

International audienceAbstract Background Microtubes (MTs), cytoplasmic extensions of glioma cells, are important cell communication structures promoting invasion and treatment resistance through network formation. MTs are abundant in chemoresistant gliomas, in particular, glioblastomas (GBMs), while they are uncommon in chemosensitive IDH-mutant and 1p/19q co-deleted oligodendrogliomas. The aim of this study was to identify potential signaling pathways involved in MT formation. Methods Bioinformatics analysis of TCGA was performed to analyze differences between GBM and oligodendroglioma. Patient-derived GBM stem cell lines were used to investigate MT formation under transforming growth factor-beta (TGF-β) stimulation and inhibition in vitro and in vivo in an orthotopic xenograft model. RNA sequencing and proteomics were performed to detect commonalities and differences between GBM cell lines stimulated with TGF-β. Results Analysis of TCGA data showed that the TGF-β pathway is highly activated in GBMs compared to oligodendroglial tumors. We demonstrated that TGF-β1 stimulation of GBM cell lines promotes enhanced MT formation and communication via calcium signaling. Inhibition of the TGF-β pathway significantly reduced MT formation and its associated invasion in vitro and in vivo. Downstream of TGF-β, we identified thrombospondin 1 (TSP1) as a potential mediator of MT formation in GBM through SMAD activation. TSP1 was upregulated upon TGF-β stimulation and enhanced MT formation, which was inhibited by TSP1 shRNAs in vitro and in vivo. Conclusion TGF-β and its downstream mediator TSP1 are important mediators of the MT network in GBM and blocking this pathway could potentially help to break the complex MT-driven invasion/resistance network