2 research outputs found
SIMULTANEOUS EFFECTS OF SHAKING AND TEMPERATURE ON VEROTOXIN1 PHAGE INDUCTION FROM VEROTOXIGENIC ESCHERICHIA COLI STRAINS
Induction of lambda phage carring verotoxin1 gene from a verotoxigenic
strains of Escherichia coli and released verotoxin1 were studied under
environmental factors of shaking and termperature. Verotoxin1 phage in
Escherichia coli PA 101 and transductants was confirmed by
bacteriophage detection assay. Shaking of culture media and increasing
temperature until 42°C increased phage particles in supernatants
of Escherichia coli PA 101. Our results indicate that environmental
factors such as shaking movements in natural inhabitates of bacteria
such as river or sewage streams and temperature rise in summer season
could be factors in induce and release free verotoxin1 –
producing phage particles in nature that in turn could be the source of
phage spreading to other related bacteria , and responsible for
increased outbreaks of food borne diseases with verotoxigenic
Escherichia coli in warm monthes of year in tropical areas