The L-amino acid oxidase of Neurospora

In 1944 one of us described a D-amino acid oxidase in extracts of Neurospora crassa (1). Except for slight activity against L-glutamate, no oxidation of L-amino acids was observed. Recently a means for inducing the formation of a soluble L-amino acid oxidase by the mold was reported by Bender, Krebs, and Horowitz (2). This is accomplished by reducing the biotin content of the basal medium (3) from the 5 γ per liter usually employed to 0.25 γ per liter. When cultured in such a medium, Neurospora produces an active L-amino acid oxidase which can be demonstrated both in extracts and in the medium. Reduction of the biotin level produces no effect on the D-amino acid oxidase, which is still found in extracts but not in the culture medium. The activity of the L-oxidase toward thirty-eight amino acids has been investigated by Bender and Krebs (4). The initial purpose of the present investigation was to explore certain possibilities for a genetic study of the enzyme. Of special interest was the fact, cited by Bender et al. (a), that the oxidase could not be detected in all of the wild type strains tested. The question arose as to whether this is due to the genetic inability of certain strains to form the enzyme. In connection with the investigation of this problem a general survey of the properties of the enzyme was carried out, together with a preliminary study of the mechanism of the biotin effect and of the adaptive formation of the enzyme. The results of these studies are reported below. Simultaneously with our investigation and independently of it, Dr. K. Burton (5), working in Professor Krebs’ laboratory, has carried out a study of the Neurospora L-amino acid oxidase. Where our respective studies overlap mutual confirmation was obtained in most essential points. We wish to thank Dr. Burton for permission to read his manuscript before publication

The Origin of Life

The origin of life is in a sense a genetic problem, for, as H. J. Muller pointed out many years ago, the essential attribute that identifies living matter is its capacity to replicate itself and its variants (1). Because this uniquely biological property has its physical basis in proteins and nucleic acids, the goal of modern work on the origin of life is to discover the manner of origin of these polymers and of the interactions between them that constitute the genetic mechanism. In attempting to review this subject in a limited space, we cannot undertake an exhaustive treatment. Rather, we summarize work published principally since 1970 in the following areas, with emphasis on those aspects that are of greatest current interest: 1. precambrian paleontology, 2. chemical evolution of genetically important monomers, 3. prebiotic dehydration-condensation reactions, 4. organic compounds in meteorites and interstellar space, and 5. biological exploration of the planets. A large number of review articles (2-5), critical and theoretical discussions (6-8), books (9-16), and conference proceedings (17-21) dealing with the origin of have appeared in recent years. In addition, a new serial, the Journal of Molecular Evolution, publishing papers on this and related subjects, appeared in 1971; the journal Space Life Sciences has been renamed "Origins of Life," and a society, the International Society for the Study of the Origin of Life, was recently founded

Photocatalytic production of organic compounds from CO and H2O in a simulated Martian atmosphere

[14C]CO2 and [14C]organic compounds are formed when a mixture of [14C]CO and water vapor diluted in [12C]CO2 or N2 is irradiated with ultraviolet light in the presence of soil or pulverized vycor substratum. The [14C]CO2 is recoverable from the gas phase, the [14C]organic products from the substratum. Three organic products have been tentatively identified as formaldehyde, acetaldehyde, and glycolic acid. The relative yields of [14C]CO2 and [14C]organics are wavelength- and surface-dependent. Conversion of CO to CO2 occurs primarily at wavelengths shorter than 2000 angstrom, apparently involves the photolysis of water, and is inhibited by increasing amounts of vycor substratum. Organic formation occurs over a broad spectral range below 3000 angstrom and increases with increasing amounts of substratum. It is suggested that organic synthesis results from adsorption of CO and H2O on surfaces, with excitation of one or both molecules occurring at wavelengths longer than those absorbed by the free gases. This process may occur on Mars and may have been important on the primitive earth

Charge-conjugation violating neutrino interactions in supernovae

The well known charge conjugation violating interactions in the Standard Model increase neutrino- and decrease anti-neutrino- nucleon cross sections. This impacts neutrino transport in core collapse supernovae through "recoil" corrections of order the neutrino energy $k$ over the nucleon mass $M$. All $k/M$ corrections to neutrino transport deep inside a protoneutron star are calculated from angular integrals of the Boltzmann equation. We find these corrections significantly modify neutrino currents at high temperatures. This produces a large mu and tau number for the protoneutron star and can change the ratio of neutrons to protons. In addition, the relative size of neutrino mean free paths changes. At high temperatures, the electron anti-neutrino mean free path becomes {\it longer} than that for mu or tau neutrinos.Comment: 14 pages, 2 included ps figures, subm. to Phys. Rev.

The ground-state of General Relativity, Topological Theories and Dark Matter

We suggest a limit of Einstein equations incorporating the state $g_{\mu\nu}=0$ as a solution. The large scale behavior of this theory has interesting properties. For a spherical source, the velocity profile for circular motions is of the form observed in galaxies (approximately flat). For FRW cosmologies, the Friedman equation contains an additional contribution in the matter sector.Comment: More clarifications on the interpretation of the limits. Shorter version. 4 pages, two column, no figure

A Correspondence Principle for Black Holes and Strings

For most black holes in string theory, the Schwarzschild radius in string units decreases as the string coupling is reduced. We formulate a correspondence principle, which states that (i) when the size of the horizon drops below the size of a string, the typical black hole state becomes a typical state of strings and D-branes with the same charges, and (ii) the mass does not change abruptly during the transition. This provides a statistical interpretation of black hole entropy. This approach does not yield the numerical coefficient, but gives the correct dependence on mass and charge in a wide range of cases, including neutral black holes.Comment: 24 pages, one typo correcte

pQCD vs. AdS/CFT Tested by Heavy Quark Energy Loss

We predict the charm and bottom quark nuclear modification factors using weakly coupled pQCD and strongly coupled AdS/CFT drag methods. The log(pT/M_Q)/pT dependence of pQCD loss and the momentum independence of drag loss lead to different momentum dependencies for the R_{AA} predictions. This difference is enhanced by examining a new experimental observable, the double ratio of charm to bottom nuclear modification factors, R^{cb}=R^c_{AA}/R^b_{AA}. At LHC the weakly coupled theory predicts R^{cb} goes to 1; whereas the strongly coupled theory predicts R^{cb} .2 independent of pT. At RHIC the differences are less dramatic, as the production spectra are harder, but the drag formula is applicable to higher momenta, due to the lower temperature.Comment: 6 pages, 4 figures. Proceedings for the International Conference on Strangeness in Quark Matter (SQM 2007), Levoca, Slovakia, 24-29 June 200

Realistic Neutrino Opacities for Supernova Simulations With Correlations and Weak Magnetism

Advances in neutrino transport allow realistic neutrino interactions to be incorporated into supernova simulations. We add tensor couplings to relativistic RPA calculations of neutrino opacities. Our results reproduce free-space neutrino-nucleon cross sections at low density, including weak magnetism and recoil corrections. In addition, our opacities are thermodynamically consistent with relativistic mean field equations of state. We find antineutrino mean free paths that are considerably larger then those for neutrinos. This difference depends little on density. In a supernova, this difference could lead to an average energy of $\bar\nu_\mu$ that is larger than that for $\nu_\mu$ by an amount that is comparable to the energy difference between $\nu_\mu$ and $\bar\nu_e$Comment: 15 pages, 10 figures, submitted to PRC, minor changes to figs. (9,10

Fusion of neutron rich oxygen isotopes in the crust of accreting neutron stars

Fusion reactions in the crust of an accreting neutron star are an important source of heat, and the depth at which these reactions occur is important for determining the temperature profile of the star. Fusion reactions depend strongly on the nuclear charge $Z$. Nuclei with $Z\le 6$ can fuse at low densities in a liquid ocean. However, nuclei with Z=8 or 10 may not burn until higher densities where the crust is solid and electron capture has made the nuclei neutron rich. We calculate the $S$ factor for fusion reactions of neutron rich nuclei including $^{24}$O + $^{24}$O and $^{28}$Ne + $^{28}$Ne. We use a simple barrier penetration model. The $S$ factor could be further enhanced by dynamical effects involving the neutron rich skin. This possible enhancement in $S$ should be studied in the laboratory with neutron rich radioactive beams. We model the structure of the crust with molecular dynamics simulations. We find that the crust of accreting neutron stars may contain micro-crystals or regions of phase separation. Nevertheless, the screening factors that we determine for the enhancement of the rate of thermonuclear reactions are insensitive to these features. Finally, we calculate the rate of thermonuclear $^{24}$O + $^{24}$O fusion and find that $^{24}$O should burn at densities near $10^{11}$ g/cm$^3$. The energy released from this and similar reactions may be important for the temperature profile of the star.Comment: 7 pages, 4 figs, minor changes, to be published in Phys. Rev.

Greybody factor for the BTZ black hole and a 5D black hole

We study the 5D black holes in the type IIB superstring theory compactified on $S^1 \times T^4$. Far from horizon, we have flat space-time. Near horizon, we have $AdS_3(BTZ black hole) \times S^3 \times T^4$. We calculate the greybody factor of a minimally coupled scalar by replacing the original geometry($M_5 \times S^1 \times T^4$) by $AdS_3 \times S^3 \times T^4$. In the low-energy scattering, it turns out that the result agrees with the greybody factor of the 5D black hole (or D1 + D5 branes)in the dilute gas approximation. This confirms that the $AdS$-theory($AdS_3 \times S^3 \times T^4$) contains the essential information about the bulk 5D black holes.Comment: some discussions are added, 15 Pages, No figure, RevTe