Helminth Infections of House Mouse (Mus musulus) and Wood Mouse (Apodemus sylvaticus) from the Suburban Areas of Hamadan City, Western Iran

Background: To determine the prevalence and intensity of helminths and their zoonotic importance in small rodents inhabiting in the suburban areas of Hamadan City,Iran. Methods: The present survey was conducted on the helminth infections of two species of rodents Apodemus sylvaticus (n=60) and Mus musculus(n=72) in the suburban areas of Hamadan City during 2010-2012. Rodents were collected and examined for helminth in the different organs. The nematodes were collected in 5% formalin solution and cleared in lactophenol, cestodes and trematodes collected from intestine fixed in AFA solution and stained by acetocarmine, cleared in xylol for identification. Results: Helminths found in A. sylvaticus and M. musculus and their prevalence for the first time in suburban areas of Hamadan City were as follows; In A. sylvaticus: Cysticercus fasciolaris(3.33%), Syphacia fredrici(26.67%), S. stroma(8.33%), Anoplocephalidae sp. (1.67%), Skrjabinotaenia lobata(5%), Plagiorchis muris(1.67%) and in M. musculus: Hymenolepis nana(16.67%), H. diminuta (5.55%), S. obvelata(30.56%), S. ohtarom (9.72%), Rodentolepis crassa(1.39%), C. fasciolaris (1.39%). Among 11 species in two rodents 4 species including S.obvelata, H. nana, H. diminuta, and P. muris have zoonotic importance. Statistically the relation between gender and their helminth infections was not significant in either M.musculus or A. sylvaticus (P>0.05). Conclusion: This study reports 11 species of helminths and on the other hand 3species were identified for the first time in Iran and 5 species of them have potential health importance for public health and cat

Histopathological Study of Esophageal Infection with Gasterophilus pecorum (Diptera: Oestridae) in Persian Onager (Equus hemionus onager)

Background: The larval stages of Gasterophilus are obligate parasites in the gastrointestinal tract of equine account­able for pathologic ulcers in the Persian onager gastrointestinal. The aim of the current report was to study the histo­pathological change with G. pecorum larvae in the esophagus of a Persian onager. Methods: This study was performed in Iranian Zebra propagation and breeding site in Khartouran National Park, southeast of Shahrud City, Semnan Province, Iran in 2014. Following a necropsy with specific refer to esophagus of one adult female Persian onager were transmitted to the laboratory. After autopsy, parasites collected from the esophagus were transmitted into 70% alcohol. For histopathological investigation, tissue samples were collected from the esophagus. The tissues were fixed in 10% buffered formalin, and conformity routine processing, there were stained with Hematoxylin and eosin. Results: After clarity by lactophenol parasites were identified as G. pecorum. Microscopic recognition contained hyperemia, inflammatory cell infiltration, epithelial destruction, esophageal gland hyperplasia. Conclusion: This is the first survey of G. pecorum and histopathological study in the Persian onager esophagus in the world