Molecular cloning and preliminary function study of iron responsive element binding protein 1 gene from cypermethrin-resistant Culex pipiens pallens

<p>Abstract</p> <p>Background</p> <p>Insecticide resistance jeopardizes the control of mosquito populations and mosquito-borne disease control, which creates a major public health concern. Two-dimensional electrophoresis identified one protein segment with high sequence homology to part of <it>Aedes aegypti </it>iron-responsive element binding protein (IRE-BP).</p> <p>Method</p> <p>RT-PCR and RACE (rapid amplification of cDNA end) were used to clone a cDNA encoding full length IRE-BP 1. Real-time quantitative RT-PCR was used to evaluate the transcriptional level changes in the Cr-IRE strain <it>Aedes aegypti </it>compared to the susceptible strain of <it>Cx. pipiens pallens</it>. The expression profile of the gene was established in the mosquito life cycle. Methyl tritiated thymidine (³H-TdR) was used to observe the cypermethrin resistance changes in C6/36 cells containing the stably transfected IRE-BP 1 gene of <it>Cx. pipiens pallens</it>.</p> <p>Results</p> <p>The complete sequence of iron responsive element binding protein 1 (IRE-BP 1) has been cloned from the cypermethrin-resistant strain of <it>Culex pipiens pallens </it>(Cr-IRE strain). Quantitative RT-PCR analysis indicated that the IRE-BP 1 transcription level was 6.7 times higher in the Cr-IRE strain than in the susceptible strain of 4th instar larvae. The IRE-BP 1 expression was also found to be consistently higher throughout the life cycle of the Cr-IRE strain. A protein of predicted size 109.4 kDa has been detected by Western blotting in IRE-BP 1-transfected mosquito C6/36 cells. These IRE-BP 1-transfected cells also showed enhanced cypermethrin resistance compared to null-transfected or plasmid vector-transfected cells as determined by ³H-TdR incorporation.</p> <p>Conclusion</p> <p>IRE-BP 1 is expressed at higher levels in the Cr-IRE strain, and may confer some insecticide resistance in <it>Cx. pipiens pallens</it>.</p

Field Measurement of Vibration Response on Large-Span Steel Structure of High-Speed Railway Station Under Multiple Load Excitations

On the basis of the health monitoring system of Taiyuan South Railway Station Steel Structure House, the measured acceleration data of the steel structure station during train arrival、departure and pass, crowd-induced excitations, and construction load excitation were analyzed, so as to evaluate the vibration response characteristics of steel structure station under different test conditions. The results show that the vertical acceleration response frequency of the structure is multiplied by train loading frequency. The response of the station under the excitation of train passing is significantly larger than that of train entering or leaving the station, and all of them will not cause significant vibration; 1 and 2 times frequency of crowd pace easily induce vertical vibration of the structure, but the Equivalent Sinusoidal Peak Acceleration (ESPA) peak of the structure does not exceed the code limit; the excitation frequency of construction load is above 10 Hz, which easily induce the vertical vibration of X-shaped steel column but the amplitude is relatively low; ESPA can better reflect the trend of acceleration amplitude. It is recommended to use this curve as a method to evaluate the comfort meas of large-span station house

Geological characteristics and potential evaluation of typical interlayer shale oil in the Ordos Basin: A case study of the Chang 7 Member of Well Ning228

The Chang 7 Member of the Ordos Basin has favorable shale oil resource potentiality. The exploration and development of sandstone interlayer shale oil in the Chang 7 Member has made substantial breakthroughs. The exploration and development potential of shale oil is huge, and will be a significant oil and gas alternative resource in the Changqing Oilfield in the future, but its related geological fundamental research is relatively weak. Based on the observation, description and systematic sampling of the centimeter-level core of the full core section of Chang 7 Member in Well Ning228, a series of experimental test analyses and identification outcomes indicated that Chang 7 Member primarily developed four rock types: Black shale, dark mudstone, siltstone and tuff. The overall conditions of source rocks in the Chang 7 member are superior, the organic carbon content of shale is high, and the hydrocarbon generation potential is large. The organic matter types are mainly type Ⅰ and type Ⅱ, which are in the low mature-mature stage, and the black shale has higher organic matter abundance. The main reservoir spaces of the Chang 7 Member are primary pores and secondary pores. Mud shale primarily develops organic pores and inorganic pores. Fine sandstone mainly develops residual intergranular pores, intragranular pores and intergranular pores. Tuff mainly develops intergranular pores and mineral intragranular pores. The overall oil-bearing property of the Chang 7 Member is good, but the mobility difference is large. The fluid mobility in sandstone and tuff is good, and the mobility inshale is poor. In short, fine anatomical analysis of typical interlayer shale oil provides favorable guidance for promoting the large-scale development of shale oil in the Ordos Basin