375 research outputs found
The biogenesis pathway of tRNA-derived piRNAs in Bombyx germ cells.
Transfer RNAs (tRNAs) function in translational machinery and further serves as a source of short non-coding RNAs (ncRNAs). tRNA-derived ncRNAs show differential expression profiles and play roles in many biological processes beyond translation. Molecular mechanisms that shape and regulate their expression profiles are largely unknown. Here, we report the mechanism of biogenesis for tRNA-derived Piwi-interacting RNAs (td-piRNAs) expressed in Bombyx BmN4 cells. In the cells, two cytoplasmic tRNA species, tRNAAspGUC and tRNAHisGUG, served as major sources for td-piRNAs, which were derived from the 5\u27-part of the respective tRNAs. cP-RNA-seq identified the two tRNAs as major substrates for the 5\u27-tRNA halves as well, suggesting a previously uncharacterized link between 5\u27-tRNA halves and td-piRNAs. An increase in levels of the 5\u27-tRNA halves, induced by BmNSun2 knockdown, enhanced the td-piRNA expression levels without quantitative change in mature tRNAs, indicating that 5\u27-tRNA halves, not mature tRNAs, are the direct precursors for td-piRNAs. For the generation of tRNAHisGUG-derived piRNAs, BmThg1l-mediated nucleotide addition to -1 position of tRNAHisGUG was required, revealing an important function of BmThg1l in piRNA biogenesis. Our study advances the understanding of biogenesis mechanisms and the genesis of specific expression profiles for tRNA-derived ncRNAs
Statistical properties of superflares on solar-type stars based on 1-min cadence data
We searched for superflares on solar-type stars using Kepler data with 1 min
sampling in order to detect superflares with short duration. We found 187
superflares on 23 solar-type stars whose bolometric energy ranges from the
order of erg to erg. Some superflares show multiple peaks
with the peak separation of the order of - seconds which is
comparable to the periods of quasi-periodic pulsations in solar and stellar
flares. Using these new data combined with the results from the data with 30
min sampling, we found the occurrence frequency () of superflares as a
function of flare energy () shows the power-law distribution () with for erg which is
consistent with the previous results. The average occurrence rate of
superflares with the energy of erg which is equivalent to X100 solar
flares is about once in 500-600 years. The upper limit of energy released by
superflares is basically comparable to a fraction of the magnetic energy stored
near starspots which is estimated from the photometry. We also found that the
duration of superflares () increases with the flare energy () as . This can be explained if we assume the time-scale of
flares is determined by the Alfvn time.Comment: Accepted for for publication in Earth, Planets and Spac
Increasing cell density globally enhances the biogenesis of Piwi-interacting RNAs in Bombyx mori germ cells.
Piwi proteins and their bound Piwi-interacting RNAs (piRNAs) are predominantly expressed in the germline and play crucial roles in germline development by silencing transposons and other targets. Bombyx mori BmN4 cells are culturable germ cells that equip the piRNA pathway. Because of the scarcity of piRNA-expressing culturable cells, BmN4 cells are being utilized for the analyses of piRNA biogenesis. We here report that the piRNA biogenesis in BmN4 cells is regulated by cell density. As cell density increased, the abundance of Piwi proteins and piRNA biogenesis factors was commonly upregulated, resulting in an increased number of perinuclear nuage-like granules where Piwi proteins localize. Along with these phenomena, the abundance of mature piRNAs also globally increased, whereas levels of long piRNA precursor and transposons decreased, suggesting that increasing cell density promotes piRNA biogenesis pathway and that the resultant accumulation of mature piRNAs is functionally significant for transposon silencing. Our study reveals a previously uncharacterized link between cell density and piRNA biogenesis, designates cell density as a critical variable in piRNA studies using BmN4 cell system, and suggests the alteration of cell density as a useful tool to monitor piRNA biogenesis and function
Physical Relation of Source I to IRc2 in the Orion KL Region
We present mid-infrared narrow-band images of the Orion BN/KL region, and
N-band low-resolution spectra of IRc2 and the nearby radio source "I." The
distributions of the silicate absorption strength and the color temperature
have been revealed with a sub-arcsecond resolution. The detailed structure of
the 7.8 micron/12.4 micron color temperature distribution was resolved in the
vicinity of IRc2. A mid-infrared counterpart to source I has been detected as a
large color temperature peak. The color temperature distribution shows an
increasing gradient from IRc2 toward source I, and no dominant temperature peak
is seen at IRc2. The spectral energy distribution of IRc2 could be fitted by a
two-temperature component model, and the "warmer component" of the infrared
emission from IRc2 could be reproduced by scattering of radiation from source
I. IRc2 itself is not self-luminous, but is illuminated and heated by an
embedded luminous young stellar object located at source I.Comment: 20 pages, 11 figures. Minor corrections had been done in the ver.2.
Accepted for publication in PAS
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