54 research outputs found
Sparsened neuronal activity in an optogenetically activated olfactory glomerulus
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 8 (2018): 14955, doi:10.1038/s41598-018-33021-w.Glomeruli are the functional units of olfactory information processing but little remains known about their individual unit function. This is due to their widespread activation by odor stimuli. We expressed channelrhodopsin-2 in a single olfactory sensory neuron type, and used laser stimulation and simultaneous in vivo calcium imaging to study the responses of a single glomerulus to optogenetic stimulation. Calcium signals in the neuropil of this glomerulus were representative of the sensory input and nearly identical if evoked by intensity-matched odor and laser stimuli. However, significantly fewer glomerular layer interneurons and olfactory bulb output neurons (mitral cells) responded to optogenetic versus odor stimuli, resulting in a small and spatially compact optogenetic glomerular unit response. Temporal features of laser stimuli were represented with high fidelity in the neuropil of the glomerulus and the mitral cells, but not in interneurons. Increases in laser stimulus intensity were encoded by larger signal amplitudes in all compartments of the glomerulus, and by the recruitment of additional interneurons and mitral cells. No spatial expansion of the glomerular unit response was observed in response to stronger input stimuli. Our data are among the first descriptions of input-output transformations in a selectively activated olfactory glomerulus.Funded by the World Class Institute/National Research Foundation of Korea (KRF: WCI 2009-003) and NIH: DC005259 and NS099691 grants
Optical analysis of circuitry for respiratory rhythm in isolated brainstem of foetal mice
Respiratory rhythms arise from neurons situated in the ventral medulla. We are investigating their spatial and functional relationships optically by measuring changes in intracellular calcium using the fluorescent, calcium-sensitive dye Oregon Green 488 BAPTA-1 AM while simultaneously recording the regular firing of motoneurons in the phrenic nerve in isolated brainstem/spinal cord preparations of E17 to E19 mice. Responses of identified cells are associated breath by breath with inspiratory and expiratory phases of respiration and depend on CO2 and pH levels. Optical methods including two-photon microscopy are being developed together with computational analyses. Analysis of the spatial pattern of neuronal activity associated with respiratory rhythm, including cross-correlation analysis, reveals a network distributed in the ventral medulla with intermingling of neurons that are active during separate phases of the rhythm. Our experiments, aimed at testing whether initiation of the respiratory rhythm depends on pacemaker neurons, on networks or a combination of both, suggest an important role for networks
Absence of Suppression in Particle Production at Large Transverse Momentum in √sNN p = 200 GeV d + Au Collisions
Transverse momentum spectra of charged hadrons with pT < 8 GeV/c and neutral pions with pT < 10 GeV/c have been measured at midrapidity by the PHENIX experiment at BNL RHIC in d + Au collisions at √sNN = 200 GeV. The measured yields are compared to those in p + p collisions at the same √sNN scaled up by the number of underlying nucleon-nucleon collisions in d + Au. The yield ratio does not show the suppression observed in central Au + Au collisions at RHIC. Instead, there is a small enhancement in the yield of high momentum particles
Azimuthal Angle Correlations for Rapidity Separated Hadron Pairs in d + Au Collisions at √sNN = 200 GeV
Absence of Suppression in Particle Production at Large Transverse Momentum in √sNN p = 200 GeV d + Au Collisions
Centrality Dependence of π0 and η Production at Large Transverse Momentum in √sNN = 200GeV d + Au Collisions
Nuclear effects on hadron production in d + Au collisions at √sNN = 200 GeV revealed by comparison with p + p data
Nuclear Modification Factors for Hadrons at Forward and Backward Rapidities in Deuteron-Gold Collisions at √sNN = 200 GeV
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