Session A3: Think Big: Adding Large Structures To Improve Ecosystem Health

Abstract: The Vindel River is one of the few freeflowing its entire catchment are part of the Natura 2000 network. The river was exploited for timber floating between 1850–1976; rapids in the main channel and tributaries below the alpine tree line were channelized to increase timber transport capacity. Side channels were cut off and the flow was concentrated to a single channel from which boulders and large wood were removed. Hence, previously heterogeneous environments were replaced by more homogeneous systems with limited habitat for riverine species. The Vindel River LIFE project (LIFE08 NAT/S/000266) works with the restoration of 25 tributaries in the Vindel River system. The project strives to alleviate the effects of fragmentation and channelization in affected rapids, to improve the quality of water and riparian habitats. The work has included the construction of over 1000 spawning grounds for brown trout, removal of 17 splash dams, the relocation of rocks into the channels, and the strategic placement of large boulders and dead wood in over 50 km of river. Follow up studies have been done in the channels that have been restored with “demonstration methods,” where previously best-practice restored reaches have been re-restored by adding large boulders and large wood (i.e., entire trees, including root wads) from adjacent upland to the channel. The demonstration restoration has generated wider and more complex streams, which in turn has led to more variable water flow and higher water levels. This will decrease the risk of erosion during high flow and reduce the risk of losing spawning areas. However, fish population data collected by electrofishing before and after restoration show very inconsistent results among tributaries. This highlights the need for considering potential catchment scale degradation and not only concentrating on reach scale problems in order to re-establish ecosystem health

Slob, a Novel Protein that Interacts with the Slowpoke Calcium-Dependent Potassium Channel

AbstractSlob, a novel protein that binds to the carboxy-terminal domain of the Drosophila Slowpoke (dSlo) calcium-dependent potassium channel, was identified with a yeast two-hybrid screen. Slob and dSlo coimmunoprecipitate from Drosophila heads and heterologous host cells, suggesting that they interact in vivo. Slob also coimmunoprecipitates with the Drosophila EAG potassium channel but not with Drosophila Shaker, mouse Slowpoke, or rat KV1.3. Confocal fluorescence microscopy demonstrates that Slob and dSlo redistribute in cotransfected cells and are colocalized in large intracellular structures. Direct application of Slob to the cytoplasmic face of detached membrane patches containing dSlo channels leads to an increase in channel activity. Slob may represent a new class of multi-functional channel-binding proteins

Astrocyte Unfolded Protein Response Induces a Specific Reactivity State that Causes Non-Cell-Autonomous Neuronal Degeneration.

Recent interest in astrocyte activation states has raised the fundamental question of how these cells, normally essential for synapse and neuronal maintenance, become pathogenic. Here, we show that activation of the unfolded protein response (UPR), specifically phosphorylated protein kinase R-like endoplasmic reticulum (ER) kinase (PERK-P) signaling-a pathway that is widely dysregulated in neurodegenerative diseases-generates a distinct reactivity state in astrocytes that alters the astrocytic secretome, leading to loss of synaptogenic function in vitro. Further, we establish that the same PERK-P-dependent astrocyte reactivity state is harmful to neurons in vivo in mice with prion neurodegeneration. Critically, targeting this signaling exclusively in astrocytes during prion disease is alone sufficient to prevent neuronal loss and significantly prolongs survival. Thus, the astrocyte reactivity state resulting from UPR over-activation is a distinct pathogenic mechanism that can by itself be effectively targeted for neuroprotection

A systematic review of the effect of therapists’ internalised models of relationships on the quality of the therapeutic relationship

The quality of the therapeutic relationship has been identified as a key factor in predicting client outcomes, accounting for around 8% of variation (Horvath, Del Re, Flückiger, & Symonds, 2011; Martin, Garske, & Davis, 2000; Wampold, 2001). Although therapist factors have been seen as less relevant to therapeutic relationship quality than client factors, focus on therapist factors has steadily increased in line with the view that the therapist responds differently to different clients, due to their own personal characteristics and unconscious processes. Relational theory suggests that the therapist’s particular qualities combine with the client’s particular qualities to form a unique interpersonal context (e.g. Wachtel, 2008). Safran and Muran (2000) suggest that the interpersonal context is heavily influenced by client and therapist internalised patterns of relating formed in early childhood. Evidence shows that certain therapist factors do affect therapeutic relationship quality; the qualities of dependability, warmth and responsiveness in therapists have all been found to create stronger alliances (Ackerman & Hilsenroth, 2003). The importance of these mostly interpersonal characteristics imply that the internalised relational models of therapists may also be important in determining the type of relationship that is built and the therapeutic processes occurring within

Media internalization and conformity to traditional masculine norms in relation to body image concerns among men

Previous studies have separately examined conformity to masculine norms and internalization of body ideals in the media in relation to the drive for muscularity (DM). This study was designed to examine these factors together in relation to DM, and further examine how they may differ in relation to drive for thinness (DT) and drive for leanness (DL). Participants were 284 Australian males between ages 18 to 42. They completed validated measures that assessed DM, DT, DL, male gender role norms, and internalization of body ideals. The findings showed that internalization of body ideals mediated the relationship between masculine role norms and body image in the case of both DM and DL. However, masculine norms and internalization were independent predictors of DT. Our findings contribute to further understanding of the roles that the media and masculine norms have in shaping men’s drive for muscularity, leanness, and thinness. Longitudinal research is needed to confirm the nature and direction of these relationships

Astrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transcriptomic map.

Although the cerebral cortex is organized into six excitatory neuronal layers, it is unclear whether glial cells show distinct layering. In the present study, we developed a high-content pipeline, the large-area spatial transcriptomic (LaST) map, which can quantify single-cell gene expression in situ. Screening 46 candidate genes for astrocyte diversity across the mouse cortex, we identified superficial, mid and deep astrocyte identities in gradient layer patterns that were distinct from those of neurons. Astrocyte layer features, established in the early postnatal cortex, mostly persisted in adult mouse and human cortex. Single-cell RNA sequencing and spatial reconstruction analysis further confirmed the presence of astrocyte layers in the adult cortex. Satb2 and Reeler mutations that shifted neuronal post-mitotic development were sufficient to alter glial layering, indicating an instructive role for neuronal cues. Finally, astrocyte layer patterns diverged between mouse cortical regions. These findings indicate that excitatory neurons and astrocytes are organized into distinct lineage-associated laminae.The study was supported by the Paul G. Allen Foundation Distinguished Investigator Program (E.M.U. and D.H.R.), the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (D.H.R., D.G. and G. C.), BRAIN initiative (1U01 MH105991 to D.G.) and National Institute of Health (1R01 MH109912 to D.G.; P01NS08351 to D.H.R.), National Institute of Health Research and the European Union Seventh Framework (to P.H.), NINDS Informatics Center for Neurogenetics and Neurogenomics (P30 NS062691 to G.C.), Wellcome Trust core support (M.H., O.A.B.), European Research Council (281961 to M.G.H.), Fonds Wetenschappelijk Onderzoek (G066715N and 1523014N to M.G.H.), Stichting Alzheimer Onderzoek (S#16025 to M.G.H.) and VIB Institutional Support and Tech Watch funding (to M.G.H.), Howard Hughes Medical Institute and the Wellcome Trust (to D.H.R.)

Child studies multiple – collaborative play for thinking through theories and methods

This text is an exploration of collaborative thinking and writing through theories, methods, and experiences on the topic of the child, children, and childhood. It is a collaborative written text (with 32 authors) that sprang out of the experimental workshop Child Studies Multiple. The workshop and this text are about daring to stay with mess, “un-closure” , and uncertainty in order to investigate the (e)motions and complexities of being either a child or a researcher. The theoretical and methodological processes presented here offer an opportunity to shake the ground on which individual researchers stand by raising questions about scientific inspiration, theoretical and methodological productivity, and thinking through focusing on process, play, and collaboration. The effect of this is a questioning of the singular academic ‘I’ by exploring and showing what a plural ‘I’ can look like. It is about what the multiplicity of voice can offer research in a highly individualistic time. The article allows the reader to follow and watch the unconventional trial-and-error path of the ongoing-ness of exploring theories and methods together as a research community via methods of drama, palimpsest, and fictionary

Body dissatisfaction and disordered eating among Portuguese and Spanish adolescents: The role of individual characteristics and internalisation of sociocultural ideals

The aim of this study was to explore the differences in patterns of risk factors for body dissatisfaction and disordered eating attitudes in both female and male adolescents from Portugal and Spain. The sample included 455 adolescents aged 12-16 years (M = 13.28, SD = 0.65) from two urban areas of each country. Body mass index, self-reported selfesteem, perfectionism, internalisation of sociocultural ideals, body dissatisfaction and disordered eating attitudes were assessed. Path analyses provided partial support for a cross-cultural model of body dissatisfaction and disordered eating in Western countries due to the presence of certain differences in the patterns of relationships across sex and country. The findings suggest the importance not only of identifying cultural specificities, even in “neighbouring” countries, but also of developing a global and comprehensive preventive approach that focuses on the influence of the ideal of beauty transmitted by Western societies

Klangen av kvinnor : Det karaktäriserande med damkörsmusik

This essay examines women's choir music from a musical perspective, to give contrast to the several previous cultural and sociological studies. The method used was a combination of literature study, an interview with former women’s choir founder and conductor Robert Sund and a music analysis of five different women’s choir pieces. The analysis showed that the sound and compositional practices in the genre stems from the challenges of the genre historically, and the uniqueness of the woman’s voice and the woman itself. Examples of these are compositional techniques like close voicings, challenging the range of the singers, or music put to lyrical themes of, for example, feminism. The challenges to the genre are something that has evolved it in the last 40 years, and in some cases things that will possibly evolve both women’s choir music further and choir music in general in the future