1 research outputs found
Peptide microarray based analysis of antibody responses to SARS-CoV-2 identifies unique epitopes with potential for diagnostic test development
Humoral immunity to the Severe Adult Respiratory Syndrome (SARS) Coronavirus (CoV)â2 is not fully understood yet but is a crucial factor of immune protection. The possibility of antibody crossâreactivity between SARSâCoVâ2 and other human coronaviruses (HCoVs) would have important implications for immune protection but also for the development of specific diagnostic ELISA tests. Using peptide microarrays, n = 24 patient samples and n = 12 control samples were screened for antibodies against the entire SARSâCoVâ2 proteome as well as the Spike (S), Nucleocapsid (N), VME1 (V), R1ab, and Protein 3a (AP3A) of the HCoV strains SARS, MERS, OC43 and 229E. While widespread crossâreactivity was revealed across several immunodominant regions of S and N, IgG binding to several SARSâCoVâ2âderived peptides provided statistically significant discrimination between COVIDâ19 patients and controls. Selected target peptides may serve as capture antigens for future, highly COVIDâ19âspecific diagnostic antibody tests