High-temperature, high-pressure optical cell

The invention is an optical cell for containment of chemicals under conditions of high temperature and high pressure. The cell is formed of a vitreous silica tube, two optical windows comprising a vitreous silica rod inserted into the ends of a tube, and fused into position in the tube ends. Windows are spaced apart to form a cavity enclosed by the tube and the windows. A hole is drilled radially through the tube and into the cavity. Another vitreous silica tube is fused to the silica tube around the hole to form the stem, which is perpendicular to the long axis of the tube. The open end of the stem is used to load chemicals into the cavity. Then the stem may be sealed, and if desired, it may be shortened in order to reduce the volume of the cavity, which extends into the stem

Structural parameters for globular clusters in NGC 5128. III. ACS surface-brightness profiles and model fits

We present internal surface-brightness profiles, based on HST/ACS imaging in the F606W bandpass, for 131 globular cluster (GC) candidates with luminosities 10^4 - 3 x 10^6 solar, in the giant elliptical galaxy NGC 5128. Several structural models are fit to the profile of each cluster and combined with mass-to-light ratios from population-synthesis models, to derive a catalogue of fundamental structural and dynamical parameters parallel in form to the catalogues recently produced by McLaughlin & van der Marel and Barmby et al. for GCs and massive young star clusters in Local Group galaxies. As part of this, we provide corrected and extended parameter estimates for another 18 clusters in NGC 5128, which we observed previously. We show that, like GCs in the Milky Way and some of its satellites, the majority of globulars in NGC 5128 are well fit by isotropic Wilson models, which have intrinsically more distended envelope structures than the standard King lowered isothermal spheres. We use our models to predict internal velocity dispersions for every cluster in our sample. These predictions agree well in general with the observed dispersions in a small number of clusters for which spectroscopic data are available. In a subsequent paper, we use these results to investigate scaling relations for GCs in NGC 5128.Comment: MNRAS, in press. 28 pages. Full data tables available at http://www.astro.keele.ac.uk/~dem/clusters.htm

Structural and Mutational Analysis of Escherichia coli AlkB Provides Insight into Substrate Specificity and DNA Damage Searching

Background: In Escherichia coli, cytotoxic DNA methyl lesions on the N1 position of purines and N3 position of pyrimidines are primarily repaired by the 2-oxoglutarate (2-OG) iron(II) dependent dioxygenase, AlkB. AlkB repairs 1-methyladenine (1meA) and 3-methylcytosine (3-meC) lesions, but it also repairs 1-methylguanine (1-meG) and 3-methylthymine (3-meT) at a much less efficient rate. How the AlkB enzyme is able to locate and identify methylated bases in ssDNA has remained an open question. Methodology/Principal Findings: We determined the crystal structures of the E. coli AlkB protein holoenzyme and the AlkBssDNA complex containing a 1-meG lesion. We coupled this to site-directed mutagenesis of amino acids in and around the active site, and tested the effects of these mutations on the ability of the protein to bind both damaged and undamaged DNA, as well as catalyze repair of a methylated substrate. Conclusions/Significance: A comparison of our substrate-bound AlkB-ssDNA complex with our unliganded holoenzyme reveals conformational changes of residues within the active site that are important for binding damaged bases. Sitedirected mutagenesis of these residues reveals novel insight into their roles in DNA damage recognition and repair. Our data support a model that the AlkB protein utilizes at least two distinct conformations in searching and binding methylated bases within DNA: a ‘‘searching’ ’ mode and ‘‘repair’ ’ mode. Moreover, we are able to functionally separate these mode

Bridging Opportunities in Human Health Services

The Campus to Community project aims to develop facilitated, in-depth site visits for VCU faculty and staff interested in exploring human health services opportunities in the Richmond community. The site visit experience will provide exposure to various community organizations specializing in human health, essentially creating a “bridge” between VCU’s campus and these facilities. This initiative is intended to motivate employees to action within the Richmond community by enabling them to observe first-hand the services that these organizations provide, learn more about the organizations’ missions, and engage in meaningful interactions with representatives on site. Likewise, it will allow Richmond community organizations to discuss unique needs and opportunities for partnerships with VCU

A 2dF spectroscopic study of globular clusters in NGC 5128: Probing the formation history of the nearest giant Elliptical

We have performed a spectroscopic study of globular clusters (GCs) in the giant elliptical NGC 5128 using the 2dF facility at the Anglo-Australian telescope. We obtained integrated optical spectra for a total of 254 GCs, 79 of which are newly confirmed on the basis of their radial velocities and spectra. In addition, we obtained an integrated spectrum of the galaxy starlight along the southern major axis. We derive an empirical metallicity distribution function (MDF) for 207 GCs (~14 of the estimated total GC system) based upon Milky Way GCs. This MDF is multimodal at high statistical significance with peaks at [Z/H]~-1.3 and -0.5. A comparison between the GC MDF and that of the stellar halo at 20 kpc (~4 Reff) reveals close coincidence at the metal-rich ends of the distributions. However, an inner 8 kpc stellar MDF shows a clear excess of metal-rich stars when compared to the GCs. We compare a higher S/N subsample (147 GCs) with two stellar population models which include non-solar abundance ratio corrections. The vast majority of our sample (~90%) appears old, with ages similar to the Milky Way GC system. There is evidence for a population of intermediate-age (~4-8 Gy) GCs (<15% of the sample) which are on average more metal-rich than the old GCs. We also identify at least one younger cluster (~1-2 Gy) in the central regions of the galaxy. Our observations are consistent with a picture where NGC 5128 has undergone at least two mergers and/or interactions involving star formation and limited GC formation since z=1, however the effect of non-canonical hot stellar populations on the integrated spectra of GCs remains an outstanding uncertainty in our GC age estimates.Comment: 17 figures, some long table

The Andromeda Project. I. Deep HST-WFPC2 V,I photometry of 16 fields toward the disk and the halo of the M31 galaxy. Probing the stellar content and metallicity distribution

HST-WFPC2 F555W and F814W photometry were obtained for 16 fields of the luminous nearby spiral galaxy M31, sampling the stellar content of the disk and the halo at different distances from the center, from ~ 20 to ~ 150 arcmin (i.e. ~ 4.5 to 35 kpc), down to limiting V and I magnitudes of ~ 27. The Color-Magnitude diagrams (CMD) show the presence of complex stellar populations, including an intermediate age/young population and older populations with a wide range of metallicity. Those fields superposed on the disk of M31 generally show a blue plume of stars which we identify with main sequence members. Accordingly, the star formation rate over the last 0.5 Gyr appears to have varied dramatically with location in the disk. All the CMDs show a prominent Red Giant Branch (RGB) with a descending tip in the V band, characteristic of metallicity higher than 1/10 Solar. A red clump is detected in all of the fields, and a weak blue horizontal branch is frequently present. The metallicity distributions (MDs), obtained by comparison of the RGB stars with globular cluster templates, are basically similar in all the sampled fields: they all show a long, albeit scantly populated metal-poor tail and a main component at [Fe/H] ~ -0.6. However, some differences also exist, e.g. in some fields a very metal-rich ([Fe/H] >= -0.2) component is present. Whereas the fraction of metal-poor stars seems to be approximately constant in all fields, the fraction of very-metal-rich stars varies with position and seems to be more prominent in those fields superposed on the disk and/or with the presence of streams or substructures. This might indicate and possibly trace interaction effects with some companion, e.g. M32.Comment: 23 pages (including 5 tables), 22 figures, submitted to A&

AphasiaBank: Preliminary Lexical, Morphosyntactic, and Error Analyses

AphasiaBank collects and analyzes samples of the discourse of individuals with aphasia and normal participants across a range of tasks. The goal of AphasiaBank is to assemble a large repository of video-recorded discourse samples, transcribed in a format that facilitates extensive computerized language analyses. This paper outlines the AphasiaBank protocol and presents core analyses of language samples from 15 normal adults and 15 individuals with aphasia using selected analyses for lexicon, morphosyntax, errors, and repetition

Structural Parameters for Globular Clusters in NGC 5128. II: HST/ACS Imaging and New Clusters

We report the first results from an imaging program with the ACS camera on HST designed to measure the structural characteristics of a wide range of globular clusters in NGC 5128, the nearest giant elliptical galaxy. From 12 ACS/WFC fields, we have measured a total of 62 previously known globular clusters and have discovered 69 new high-probability cluster candidates not found in any previous work. We present magnitudes and color indices for all of these, along with rough measurements of their effective diameters and ellipticities. The luminosity distribution of this nearly-uncontaminated sample of clusters matches well with the normal GCLF for giant elliptical galaxies, and the cluster scale size and ellipticity distributions are similar to those in the Milky Way system. The indication from this survey is that many hundreds of individual clusters remain to be found with carefully designed search techniques in the future. A very rough estimate of the total cluster population from our data suggests N_GC = 1500 in NGC 5128, over all magnitudes and within a projected radius R = 25' from the galaxy center.Comment: AASTex, 33 preprint pages including 9 Figures. Accepted for publication in Astronomical Journal, volume 132 (2006