52 research outputs found
Avaliação da lesĂŁo isquĂŞmica normotĂ©rmica do fĂgado: papel da oclusĂŁo do ducto biliar principal e da N-acetilcisteĂna
Effect of Dietary Inclusion of N-Acetyl Cysteine on Mucus Viscosity and Susceptibility of Rainbow Trout, Oncorhynchus mykiss, and Atlantic Salmon, Salmo salar, to Amoebic Gill Disease
A synthetic mammalian gene circuit reveals antituberculosis compounds
Synthetic biology provides insight into natural gene-network dynamics and enables assembly of engineered transcription circuitries for production of difficult-to-access therapeutic molecules. In Mycobacterium tuberculosis EthR binds to a specific operator (OethR) thereby repressing ethA and preventing EthA-catalyzed conversion of the prodrug ethionamide, which increases the resistance of the pathogen to this last-line-of-defense treatment. We have designed a synthetic mammalian gene circuit that senses the EthR–OethR interaction in human cells and produces a quantitative reporter gene expression readout. Challenging of the synthetic network with compounds of a rationally designed chemical library revealed 2-phenylethyl-butyrate as a nontoxic substance that abolished EthR's repressor function inside human cells, in mice, and within M. tuberculosis where it triggered derepression of ethA and increased the sensitivity of this pathogen to ethionamide. The discovery of antituberculosis compounds by using synthetic mammalian gene circuits may establish a new line of defense against multidrug-resistant M. tuberculosis
Pharmacokinetics of N-acetylcysteine following repeated intravenous infusion in haemodialysed patients
Comparative study of rifampicin pharmacokinetics administered orally and intravenously in the fasted and non-fasted rats
Thermogravimetry/gas chromatography/mass spectrometry and thermogravimetry/gas chromatography/Fourier transform infrared spectroscopy: Novel hyphenated methods in thermal analysis
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