HPLC Determination and Pharmacokinetic Study of Homoeriodictyol-7-O-â- D- glucopyranoside in Rat Plasma and Tissues

Homoeriodictyol-7-O-β-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, 1H- and 13C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone. Vanillin was selected as internal standard. The mobile phase consisted of methanol–water–glacial acetic acid (45 : 55 : 0.5, v/v/v). Good linearity were observed over the concentration ranges of 0.1—200.0 μg·ml−1 in rat plasma and 0.05—5.0 μg·ml−1 in tissues. Both intra- and inter-day precisions of HEDT-Glu, expressed as the relative standard deviation, were less than 13.1%. Accuracy, expressed as the relative error, ranged from −0.8 to 5.4% in plasma and from −5.6 to 9.4% in tissues. The mean extraction recovery of HEDT-Glu was above 73.17% in biological samples. The described assay method was successfully applied to the pre-clinical pharmacokinetic study of HEDT-Glu. After intravenous administration of HEDT-Glu to rat, AUC and CLtot were 16.04±3.19 μg·h·ml−1 and 0.85±0.17 l·kg−1·h−1, respectively. T1/2,α and t1/2,β were 0.06±0.01 h and 1.27±0.31 h, respectively. HEDT-Glu was cleared from the blood and mainly distributed to the liver and small intestine

Illustrierte Flora von Mittel-Europa : mit besonderer Berücksichtigung von Deutschland, Österreich und der Schweiz /

Zum Gebrauche in den Schulen und zum Selbstunterricht1. 1. Monocotyledones : Pteridophyta, Gymnospermae und Monocotyledones / bearb. und hrsg. von Karl Suessenguth unter Mitw. von Ernst Bergdolt e. a.2. Monocotyledones3. 1. Dicotyledons / hrsg. von Karl-Heinz Rechinger unter Mitarb. von Annelis Schreiber3. 3. Dicotyledons : Nymphaeceen, Ceratophyllaceen, Magnoliaceae Paeoniaceen, Ranunculaceen / hrsg. von Karl-Heinz Rechinger und Jurgen Damboldt4. 1. Dicotyledons : Berberidaceae, Lauraceae, Rhoeadales / hrsg. von Friedrich Markgraf unter Mitarb. von L. Hoerhammer e. a.4. 2. Dicotyledons : Droseraceae, Philadelphaceae, Grossulariaceae, Crassulaceae, Saxifragaceae, Parnassiaceae, Rosaceae / hrsg. von Herbert HuberBd.III-VI. Dicotyledons6. 1. Dicotyledons : Scrophulariaceae, Orobanchaceae, Lentibulariaceae, Globulariaceae, Plantaginaceae / hrsg. von Dimitri Hartl und Gerhard WagenitzBd.VII. Gesamtregiste